The Effect of Methanol and Ethyl Acetate Extracts of Kirinyuh Stem (Eupatorium odoratum L.) on Antifungal Activity of Fusarium oxysporum
DOI:
https://doi.org/10.26555/bioedukatika.v11i2.30900Keywords:
Fusarium oxysporum, Eupatorium odoratum L., Metanol, Etil AsetatAbstract
Fusarium oxysporum is a fungus that has a negative role, namely causing moler disease or Fusarium wilt in shallot plants. The purpose of this study was to determine the effect of methanol and ethyl acetate extracts of kirinyuh stem (Eupatorium odoratum L.) on the antifungal activity of Fusarium oxysporum. This study used a complete randomized design (CRD) with 8 treatments, namely negative control, positive control, concentrations of 10%, 20%, 40%, 60%, 80%, 100% with 3 replicates. The activity test was conducted using the Kirby Bauer method. Data from the study were processed by analysis of variance (one way anova) and continued by Duncan's test. The results showed that methanol and ethyl acetate extracts of kirinyuh stems affect the antifungal activity of Fusarium oxysporum. Ethyl acetate extract is more active to inhibit Fusarium oxysporum fungi compared to methanol extract. The highest inhibition zone diameter in the methanol extract was at 100% concentration which was 7.5 mm and the lowest at 10% concentration which was 2.67 mm. The highest inhibition zone diameter in the ethyl acetate extract was at 100% concentration which was 12.33 mm and the lowest at 10% concentration which was 4.67 mm.
References
Alioes, Y., Kartika, A., Zain, E. A., & Azzura, V. (2018). Uji Potensi Antijamur Candida albicans Ekstrak Daun Gelinggang (Cassia alata L.) Dibandingkan Dengan Sediaan Daun Sirih yang Beredar Di Pasaran Secara in Vitro. Jurnal Kimia Riset, 3(2), 108–115. https://doi.org/10.20473/jkr.v3i2.12040
Anjaswati, D., Pratimasari, D., & Nirwana, A. P. (2021). Perbandingan Rendemen Ekstrak Etanol, Fraksi N-Heksana, Etil Asetat, dan Air Daun Bit (Beta vulgaris L.) Menggunakan Fraksinasi Bertingkat. Stikes, 1(1), 1–6.
Ariyanti, N. K., Darmayasa, I. B. G., & Sudirga, S. K. (2012). Daya Hambat Ekstrak Kulit Daun Lidah Buaya (Aloe barbadensis Miller) terhadap Pertumbuhan Bakteri Staphylococcus aureus ATCC 25923 dan Escherichia coli ATCC 25922. Jurnal Biologi, 16(1), 1–4. http://ojs.unud.ac.id/index.php/bio/article/download/5301/4057
Aviany, H. B., & Pujiyanto, S. (2020). Analisis Efektivitas Probiotik di dalam Produk Kecantikan sebagai Antibakteri terhadap Bakteri Staphylococcus epidermidis. Jurnal Berkala Bioteknologi, 3(2), 24–31.
Azmi, F., Chatri, M., Advinda, L., & Irdawati. (2021). Effect of Rambutan Leaf Extract (Nephelium lappaceum L.) on Colony Diameter and Percentage of Growth of Inhibition Fusarium oxysporum. Serambi Biologi, 6(1), 7–11.
Bayuaji, T. S., Astuti, I. Y., & Dhiani, B. A. (2012). Aktivitas Antifungi Krim Daun Ketepeng Cina (Senna alata L. Roxb.) terhadap Trichophyton mentagrophytes. Pharmacy, 9(3), 57–64.
Firdaus, R., Ardiningsih, P., & Arreneuz, S. (2015). Aktivitas Antijamur Ekstrak Teripang Butoh Keling (Holothuria leucospilota) Dari Pulau Lemukutan terhadap Candida albicans. Jurnal Kimia Khatulistiwa, 4(4), 7–14.
Gultom, E. S., Sakinah, M., & Hasanah, U. (2020). Eksplorasi Senyawa Metabolit Sekunder Daun Kirinyuh (Chromolaena odorata) dengan GC-MS. Jurnal Biosains, 6(1), 23–26.
Herdiana, I., & Aji, N. (2020). Fraksinasi Ekstrak Daun Sirih dan Ekstrak Gambir serta Uji Antibakteri Streptococcus mutans. Jurnal Ilmiah Kesehatan, 19(3), 100–106. https://doi.org/10.33221/jikes.v19i03.580
Intan, K., Diani, A., & Nurul, A. S. R. (2021). Aktivitas Antibakteri Kayu Manis (Cinnamomum burmanii) terhadap Pertumbuhan Staphylococcus aureus. Jurnal Kesehatan Perintis, 8(2), 121–127. https://doi.org/10.33653/jkp.v8i2.679
Jihad, A. F. A., Zulfa, F., & Bahar, M. (2020). Uji efektivitas ekstrak bawang bombai (Allium cepa L. Var. Cepa) terhadap pertumbuhan jamur Mallasezia furfur secara in vitro. Seminar Nasional Riset Kedokteran, 295–303.
Juliantari, N. P. D., Wrasiati, L. P., & Wartini, N. M. (2018). Karakteristik Ekstrak Ampas Kopi Bubuk Robusta (Coffea canephora) Pada Perlakuan Konsentrasi Pelarut Etanol dan Suhu Maserasi. Jurnal Rekayasa Dan Manajemen Agroindustri, 6(3), 243–249. https://doi.org/10.24843/jrma.2018.v06.i03.p08
Juwanda, M., Khotimah, H., & Amin, M. (2016). Peningkatan Ketahanan Bawang Merah terhadap Penyakit Layu Fusarium Melalui Induksi Ketahanan Dengan Asam Salisilat Secara In Vitro. Agrin, 20(1), 15–28.
Kandoli, F., Abijulu, J., & Leman, M. (2016). Uji Daya Hambat Ekstrak Daun Durian (Durio zybethinus) terhadap Pertumbuhan Candida albicans Secara In Vitro. Jurnal Ilmiah Farmasi, 5(1), 46–52.
Komala, O., . Y., & Siwi, F. R. (2020). Aktivitas Antijamur Ekstrak Etanol 50% dan Etanol 96% Daun Pacar Kuku Lawsonia inermis L terhadap Trichophyton mentagrophytes. Ekologia: Jurnal Ilmiah Ilmu Dasar Dan Lingkungan Hidup, 19(1), 12–19. https://doi.org/10.33751/ekol.v19i1.1657
Kurang, R. Y., & Penlaana, R. (2022). Daya Hambat Ekstrak Metanol dan Etil Asetat Daun Kirinyuh (Chromolaena odorata L.) terhadap Bakteri Escherichia Coli. Jamb.J.Chem, 4(2), 22–29.
Nurdin, E., & Nurdin, G. M. (2020). Perbandingan Variasi Media Alternatif dengan Berbagai Sumber Karbohidrat Terhadap Pertumbuhan Candida albicans. Bionature, 21(1), 1–5. https://doi.org/10.35580/bionature.v21i1.13920
Putra, I. M. A. S. (2015). Uji Aktivitas Antibakteri Ekstrak Etanol Daun Sirsak (Annonae muricata L.) dengan Metode Difusi Agar Cakram terhadap Escherichia coli. Jurnal Ilmiah Medicamento, 1(1), 15–19. https://doi.org/10.36733/medicamento.v1i1.721
Putri, A., Nofita, N., & Ulfa, A. M. (2022). Perbandingan Aktivitas Antioksidan Ekstrak Daun Bidara (Ziziphus spina-christi L.) dengan Teknik Ekstraksi Perkolasi dan Infusa. Jurnal Ilmu Kedokteran dan Kesehatan, 9(4), 1178–1189. https://doi.org/10.33024/jikk.v9i4.5635
Putri, D. R., Asri, M. T., & Ratnasari, E. (2019). Aktivitas Antifungi Ekstrak Buah Pare (Momordica charantia L.) dalam Menghambat Pertumbuhan Jamur Fusarium oxysporum. Lentera Bio, 8(2), 156–161. http://ejournal.unesa.ac.id/index.php/lenterabio
Putri, W. S., Warditiani, N. K., & Larasanty, L. P. F. (2013). Skrining Fitokimia Ekstrak Etil Asetat Kulit Buah Manggis (Garcinia mangostana L.). Jurnal Farmasi Udayana, 2(4), 56–60.
Rahmi, N., Salim, R., Miyono, & Rizki, M. I. (2021). Pengaruh Jenis Pelarut dan Metode Ekstraksi terhadap Aktivitas Antibakteri dan Penghambatan Radikal Bebas Ekstrak Kulit Kayu Bangkal (Nauclea subdita). Jurnal Penelitian Hasil Hutan, 39(1), 13–26. https://doi.org/10.20886/jphh.2021.39.1.13-26
Salamah, N., & Widyasari, E. (2015). Aktivitas Antioksidan Ekstrak Metanol Daun Kelengkeng (Euphoria longan (L) Steud.) dengan Metode Penangkapan Radikal 2,2’-Difenil-1-Pikrilhidrazil. Pharmaciana, 5(1), 25–34. https://doi.org/10.12928/pharmaciana.v5i1.2283
Sari, E. M., Suwirmen, & Noli, Z. A. (2014). Pengaruh Penggunaan Fungisida (Dithane M-45) terhadap Pertumbuhan Tanaman Jagung (Zea mays L.) dan Kepadatan Spora Fungi Mikoriza Arbuskula (FMA). Jurnal Biologi Universitas Andalas (J. Bio. UA.), 3(3), 188–194.
Setyantoro, M. E., Haslina, H., & Wahjuningsih, S. B. (2019). Pengaruh Waktu Ekstraksi dengan Metode Ultrasonik terhadap Kandungan Vitamin C, Protein, dan Fitokimia Ekstrak Rambut Jagung (Zea mays L.). Jurnal Teknologi Pangan dan Hasil Pertanian, 14(2), 53–67. https://doi.org/10.26623/jtphp.v14i2.2445
Suryani, Y., Taupiqurrahman, O., & Kulsum, Y. (2020). Mikologi. PT. Freeline Cipta Granesia.
Tusa’diah, H., & Chatri, M. (2021). Uji Efektivitas Ekstrak Daun Sukun (Artocarpus altilis Park.) terhadap Koloni Jamur Fusarium oxysporum Secara In-Vitro. Prosiding Semnas Bio, 1679–1685. https://semnas.biologi.fmipa.unp.ac.id/index.php/prosiding/article/view/215/147
Wahyuni, & Karim, S. F. (2020). Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kacapiring (Gardenia jasminoides Ellis) terhadap Bakteri Streptococcus mutans. Jurnal Sains Dan Kesehatan, 2(4), 399–404.
Warbung, Y. Y., Wowor, V. N. S., & Posangi, J. (2013). Daya Hambat Ekstrak Spons Laut Callyspongia sp terhadap Pertubuhan Bakteri Staphylococcus aureus. E-Gigi, 1(2), 1–12. https://doi.org/10.35790/eg.1.2.2013.3151
Warni, J., Marliah, A., & Erida, G. (2022). Uji Aktivitas Bioherbisida Ekstrak Metanol Teki (Cyperus rotundus L.) terhadap Pertumbuhan Bayam Duri (Amaranthus spinosus L.). Jurnal Ilmiah Mahasiswa Pertanian, 7(2), 47–54. https://doi.org/10.17969/jimfp.v7i4.22071
Widiastuti, A., Agustina, W., Wibowo, A., & Sumardiyono, C. (2011). Uji Efektivitas Pestisida terhadap Beberapa Patogen Penyebab Penyakit Penting pada Buah Naga (Hylocereus sp.) secara In Vitro. Jurnal Perlindungan Tanaman Indonesia, 17(2), 73–76.
Yanti, N., Samingan, & Mudatsir. (2016). Uji Aktivitas Antifungi Ekstrak Etanol Gel Manjakani (Quercus infectoria) terhadap Candida albicans. Jurnal IImiah Mahasiswa Pendidikan Biologi, 1(1), 1–9.
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