Antioxidant activity assay of white Turi (Sesbania grandiflora (L.) Pers.) extracts using DPPH radical scavenging method

Authors

  • Jamilatur Rohmah Medical Laboratory TechnologyFaculty of Health Science , Universitas Muhammadiyah Sidoarjo http://orcid.org/0000-0002-4748-3375
  • Ida Agustini Saidi Department of Agricultural Product Technology, Faculty of Science and Technology Universitas Muhammadiyah Sidoarjo
  • Chylen Setiyo Rini Medical Laboratory TechnologyFaculty of Health Science , Universitas Muhammadiyah Sidoarjo
  • Zulfida Ainindiyah Putri Purwanto Postgraduate Program of Medical Laboratory Technology, Faculty of Health Science, Universitas Muhammadiyah Sidoarjo
  • Karlia Hedit Tiana Postgraduate Program of Medical Laboratory Technology, Faculty of Health Science, Universitas Muhammadiyah Sidoarjo
  • Tri Chahyani Rahmawati Putri Postgraduate Program of Medical Laboratory Technology, Faculty of Health Science, Universitas Muhammadiyah Sidoarjo

DOI:

https://doi.org/10.12928/pharmaciana.v10i3.16643

Keywords:

antioxidant activity, white turi (Sesbania grandiflora (L.) Pers.) leaves extract, DPPH, various extraction solvents

Abstract

Turi (Sesbania grandiflora (L.) Pers.) is a type of plant belonging to the Fabaceae family that is widespread in Indonesian territory and has the potential as a natural free radical scavenger. The study aims to compare the antioxidant activity from white Turi (Sesbania grandiflora (L.) Pers.) leaf extracts in various extraction solvents. The determination of antioxidant activity was carried out using the DPPH method with a UV-Vis spectrophotometer. The turi plants used were originated from Mojosari, Mojokerto, East Java, Indonesia. Simplicia turi leaves were macerated in various extraction solvents: ethanol, ethyl acetate, and n-hexane for 24 hours. The extraction results are then subjected to phytochemical and antioxidant activity tests. The obtained result shows that the white turi extract has antioxidant activity with strong activity against DPPH radicals; with n-hexane > ethyl acetate extract > ethanol extract. The IC50 values of ethanol, n-hexane, and ethyl acetate extracts of white turi leaves were 33.09 ppm; 26.99 ppm; and 25.33 ppm. Based on these results, the white turi plant (leaves) can be one of the natural sources of antioxidants to prevent diseases caused by free radicals.

Author Biography

Jamilatur Rohmah, Medical Laboratory TechnologyFaculty of Health Science , Universitas Muhammadiyah Sidoarjo

Medical Laboratory Technology

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Published

2020-10-28

Issue

Vol. 10 No. 3 (2020): Pharmaciana

Section

Analytical Pharmacy and Medicinal Chemistry

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