Total phenol, flavonoid, and anthocyanin content and antioxidant activity of Etlingera elatior extract and nanoparticle

Authors

  • Tresna Lestari Sekolah Tinggi Ilmu Kesehatan Bakti Tunas Husada
  • Tita Nofianti Sekolah Tinggi Ilmu Kesehatan Bakti Tunas Husada
  • Lilis Tuslinah Sekolah Tinggi Ilmu Kesehatan Bakti Tunas Husada
  • Ruswanto Ruswanto Sekolah tinggi ilmu kesehatan bakti tunas husada
  • Yulia Salmini Sekolah Tinggi Ilmu Kesehatan Bakti Tunas Husada
  • Dewi Dewi Sekolah Tinggi Ilmu Kesehatan Bakti Tunas Husada
  • Leli Siti Zaqiah Sekolah Tinggi Ilmu Kesehatan Bakti Tunas Husada
  • Anggi Agustira Sekolah Tinggi Ilmu Kesehatan Bakti Tunas Husada
  • Putri Pratiwi Sekolah Tinggi Ilmu Kesehatan Bakti Tunas Husada

DOI:

https://doi.org/10.12928/pharmaciana.v8i1.7511

Keywords:

ginger flower extract, nanoparticle, antioxiandt

Abstract

Despite the long and wide application, traditional medicine is known for its minimum efficacy. Nanoparticle technology has reported to optimally address this weakness by enhancing the ability of the medicine to penetrate the biological membrane and, thereby, increasing the absorption. In this research, Etlingera elatior (ginger flower) extract, which has antioxiandt activity, was formulated into nanoparticles with ionotropic gelation method using chitosan (0.08, 0.09, 0.1, 0.2, and 0.3%) and 0.01% NaTPP. The nanoparticles were characterized by their particle size, polydispersity index, zeta potential, and entrapment efficiency against total phenolic compound, flavonoid, and anthocyanin. The ones with the best properties were then analyzed with Scanning Electron Microscopic (SEM) method and tested for its antioxidant activity against DPPH. The results showed that all of the formula variations produced particle size in the range of 147.0-566.2 nm with a polydispersity index of < 0.5 and zeta potential between 0.45-45.90 mV. Also, the absorption efficiencies of phenol, flavonoid, and anthocyanin were 72.62-84.24%, 55.18-92.05%, and 75.67-97.96%, respectively. Overall, the best characteristics were presented by the combination of 0.1% chitosan and 0.01% NaTPP, which produced 246.4-nm nanoparticles with a polydispersity index of 0.418, and zeta potential of 26.60 mV. These nanoparticles also contained phenol, flavonoid, and anthocyanin with good absorption efficiencies, namely 78.5186%, 92.05%, and 97.96%, respectively. SEM analysis showed that these nanoparticles were round and had a soft surface. The radical scavenging activities of the extract and the nanoparticles against DPPH, as presented by the IC50 values, were 19.614 ppm and 160 ppm.

References

Adliani, Nur., Nazliniwaty dan Djendakita, 2012, Formulasi Lipstik Zat Warna Dari Ekstrak Bunga Kecombrang (Etlingera elatior (Jack) R. M. Sm), Journal of Pharmaceutics and Pharmacology, 1 (2), 87 – 94.

Ahmad R.A, Juwita, D.A Ratulangi, 2015, Penetapan Kadar Fenolik dan Flavonoid Total Ekstrak Metanol Buah dan Daun Patikala (Etlingera elatior (jack) R.M.SM). Pharm Sci Ress, 2 (1), 1-10.

Carlsen M.H, Bente L.H, Kari Holte, Siv K.B, Steinar Dragland, et. al, 2010, The total antioxidant content of more than 3100 foods, beverages, spices, herbs and supplements used worldwide, Nutrition Journal, 9(3).

Chattopadhyay DP, Inamdar MS. 2010. Aquoeus behavior of chitosan. Interantional Journal of Polymer Science, 2010 (2010), 1-7.

Firmawan F, 2012, Pembuatan dan Karakterisasi Sediaan Nanopartikel Salbutamol Sulfat, Tesis, Program Studi Sains dan Teknologi Farmasi, Institut Teknologi Bandung

Gao, L., D. Zhang, and M. Chen, 2008, Drug nanocrystals for formulation of poorly soluble drugs and its application as potential drug delivery system, J. Nanopart Res, 10 (1), 845 – 862.

Giusti, M.M., dan Worlstad, R.E., 2001, Characterization and Measurement of Anthocyanins by UV-Visible Spectroscopy, Oregon State University.

Iswandana, Anwar, Jufri. 2013. Formulasi Nanopartikel Verapamil Hidroklorida dari Kitosan dan Natrium Tripolifosfat dengan Metode Gelasi Ionik. Jurnal Farmasi Indonesia, 6 (4), 201-210

Kementerian Kesehatan RI, 2011, Suplemen II Farmakope Herbal Indonesia, Edisi I. Jakarta: Kementrian Kesehatan Republik Indonesia.

Lestari, A. 2014. Uji aktivitas antioksidan ekstrak bunga kecombrang (Etlingera elatior (Jack) R.M.Sm.) dengan variasi pelarut polar terhadap radikal bebas DPPH (1,1-diphenyl-2-picrylhidrazyl). Skripsi. Program Studi S1 Farmasi. STIKes Bakti Tunas Husada Tasikmalaya.

Lestari T., Ruswanto. 2015. Fitokimia Total Phenolic Content dan Sitotoksik Ekstrak dan Minyak Atsiri Bunga Kecombrang (Etlingera elatior). Seminar Nasional dan Diseminasi Penelitian Kesehatan. STIKes Bakti Tunas Husada. Tasikmalaya.

Mohanraj, VJ., Y. Chen, 2006, Nanoparticles – A Review, Trop J Pharl Res, 5(1), 561-573.

Munawaroh, S. 2014. Pengaruh Metode Dan Variasi Pelarut Ekstraksi Terhadap Kadar Polifenolat Total Bunga Kecombrang (Etlingera Elatior (Jack) R.M.Sm). Skripsi. Program Studi S1 Farmasi. STIKes Bakti Tunas Husada Tasikmalaya.

Mukhriani, Faridha Yenny Nonci, Mumang, 2014, Penetapan Kadar Tanin Total Ekstrak Biji Jintan Hitam (Nigella sativa) Secara Spektrofotometri UV-VIS. Skripsi. Jurusan Farmasi Fakultas Ilmu Kesehatan Universitas Islam Negeri Alauddin Makassar.

Patil, J.S., M.V. Kamalapur, S.C. Marapur, D.V. Kadam, 2010, Ionotropic Gelation and Polyelectrolyte Complexation : The Novel Thechniques to Design Hydrogel Particulate Sustained, Modulated Drug Delivery System : A Review, Digest Journal of Nanomaterial and Biostructures, 5(1), 241-248.

Tilak, J. C dan Devasagayam, T. P. A, 2006, Oxidative Damage to Mitochondria. In Singh, K. K., editor. Oxidative Stress, Disease and Cancer, Singapura: Mainland Press, 85-150.

Ukieyanna, E. 2012. Aktivitas antioksidan, Kadar Fenolik dan Flavonoid Total Tumbuhan Suruhan (Peperomia pellucida L. Kunth.). Skripsi. Departemen Biokimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor

Windono T, Riyanto B, Ivone, Sherly V dan Yovita S, 2004, Studi Hubungan Struktur-Aktivitas Kapasitas Peredaman Radikal Bebas Senyawa Flavonoid Terhadap 1,1-difenil-2-pikrilhidrazil (DPPH), Artocarpus, 4(2), 48.

Won J., M.-H. Oh, J.-M. Oh, M.-S. Kang, J.-H. Choy, and S. Oh. 2008, Stability Analysis of Zinc Oxide-Nanoencapsulated Conjugated Linoleic Acid and Gamma-Linolenic Acid, Journal of Food Science, 73(8), 39–43.

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Published

2018-06-02

Issue

Vol. 8 No. 1 (2018): Pharmaciana

Section

Biology Pharmacy

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