VALIDASI METODE ANALISA PENETAPAN KADAR EPIGALOKATEKIN GALAT DENGAN KLT DENSITOMETRI

Authors

  • Nining Sugihartini Fakultas Farmasi UAD
  • Achmad Fudholi Fakultas Farmasi UGM
  • Suwidjiyo Pramono Fakultas Farmasi UGM
  • Sismindari Sismindari Fakultas Farmasi UGM

DOI:

https://doi.org/10.12928/pharmaciana.v2i1.656

Keywords:

epigallocathechin gallate, validation, KLT Densitometry method

Abstract

TLC Densitometry is one of method which was used to measure amount of active
substance. Active substance of epigallocathechin gallate is one of in green tea extract
cream. Method of analysis must be validated to prove that method will give the data
close with the real value. Aim of this research is to prove that TLC Densitometry method
have liniearity, precision and accuration that fulfill the requirement. This study used
concentration 600, 1800 and 3000 ug/ml with 3 replications to measure precision
(based on value of CV) and accuration (based on value of recovery). Liniearity was
known based on value of r of curve regression between concentration and wide area of
chromatogram. LOD and LOQ was calculated based on curve regression. Research
showed that method of analyse had liniearity with r= 0,98. Value of CV concentration
of 600, 1800 and 3000 ug/ml were 8,18%; 3,19% and 1,53%, respectively and recovery
were 88,10%; 99,65% dan 111,33%, respectively. Value of LOD was 827,01 µg/ml and
LOQ was 2756,69 µg/ml.

Downloads

Published

2012-05-01

Issue

Vol. 2 No. 1 (2012): Pharmaciana

Section

Analytical Pharmacy and Medicinal Chemistry

License

Authors who publish with Pharmaciana agree to the following terms:

  1. Authors retain copyright and grant the journal the right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA 4.0) that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal. 
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.