UJI SITOTOKSITAS DAN ANTIPROLIFERATIF SEL KANKER PAYUDARA T47D DAN SEL VERO BIJI Nigella sativa, L.

Authors

  • Laela Hayu Nurani Fakultas Farmasi UAD

DOI:

https://doi.org/10.12928/pharmaciana.v2i1.637

Keywords:

Seeds of Nigella sativa, L., cytotoxicity, antiproliferative, T47D breast cancer cells, Vero cells

Abstract

Black cumin seeds contain oil classes atisiri, terpenes, and alkaloids that can be
used for traditional medicine as anticancer. The purpose of this study was to assess the
cytotoxic effects of ether extract, ethanol, and infusa seed Nigella sativa, L. (Black
cumin) to inhibit the growth of T47D and normal (Vero) cells and its effect on the
kinetics of T47D breast cancer cells proliferation. This study were used extracts of
ether, ethanol, and black cumin seeds infusa that obtained by maceration method and
infundasi. Cytotoxicity test was performed by incubating T47D breast cancer cells at a
2 x 104 density with concentrations series of 2000; 1000; 500: 250: 125; 62.5; 31.25
and 15.625 microg/ml for 24 hours. Vero cell with a density of 2 x 104 with
concentrations series of 4000; 2000; 1000; 500; 250; 125 and 62.5 microg/ml. The
number of cells was calculated by direct counting method and the calculated the death
percentage. The LC50 values calculated using probit analysis. Observations on the
nature of the growth inhibition were done by observing kinetics of cell proliferation
with the addition of trypan blue at-24, 48 and 72 to determine its doubling time. The
results showed that the ether extract, ethanol, and black cumin seeds infusa are
cytotoxic to T47D breast cancer cells with successive LC50 of 32.63: 10.02, and 23.82
mg mL. Vero cell cytotoxicity test to produce successive LC50 of 300.6; 328.41, and
778.64 g/ml. Antiproliferative test results showed that in 62.5 ug/ml and 31.625
microg/ml prolong the doubling time. Ethanol extract of cumin seeds have a higher
potential due to the highest security index compared to ether extract and infusa.

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Published

2012-05-01

Issue

Vol. 2 No. 1 (2012): Pharmaciana

Section

Analytical Pharmacy and Medicinal Chemistry

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