EFEK SITOTOKSIK DAN PEMACUAN APOPTOSIS FRAKSI PETROLEUM ETER EKSTRAK ETANOL DAUN TAPAK LIMAN (Elephantopus scaber Linn) TERHADAP SEL HELA

Authors

  • Yeni Listyowati Farmasi UAD
  • Nurkhasanah Nurkhasanah Farmasi UAD

DOI:

https://doi.org/10.12928/pharmaciana.v3i2.424

Keywords:

Elephantopus scaber Linn, cytotoxicity, apoptosis, HeLa cells

Abstract

Elephantopus scaber Linn. has been reported to have cytotoxic effects on breast cancer cells and the
potential to be developed as anticancer agent. This study aims was to determine the cytotoxic activity and
apoptosis induction effect of petroleum ether fractions of ethanolic extract of (Elephantopus scaber Linn) leaves
against cervical cancer cells (HeLa). Petroleum ether fraction was obtained by dissolving eyhanolic extract in
petroleum ether, and the soluble fraction was as petroleum ether fraction. The method used for cytotoxic activity
test was MTT test. The concentration series used were 2000; 1500; 1000; 800; 400; 200; 100; 50; 25; 12.5; 6.25
and 3.125 mg/ml. The IC50 used as cytotoxic parameters. The apoptotic observations was conducted using
acridine orange and ethidium bromide. The study showed the IC50 of petroleum ether fraction of (Elephantopus
scaber Linn) ethanolic extract was 185 ug/ml. The study also showed the potency to stimulate apoptosis in HeLa
cells.

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Published

2013-11-01

Issue

Vol. 3 No. 2 (2013): Pharmaciana

Section

Analytical Pharmacy and Medicinal Chemistry

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