PENGARUH NATTOKINASE TERHADAP DAYA KERJA Metformin HCl DALAM MENURUNKAN KADAR GLUKOSA DARAH PADA TIKUS PUTIH JANTAN (Rattus norvegicus) GALUR WISTAR

Authors

  • M Sandila Okta Farmasi UAD
  • Vivi Sofia Farmasi UAD

DOI:

https://doi.org/10.12928/pharmaciana.v3i1.420

Keywords:

Nattokinase, drug interactions, diabetes mellitus

Abstract

Interaction of modern drug and traditional drug is an issue that needs to be
examined, not least in the treatment of diabetes mellitus. Nattokinase is a nutraceutical
product that can be used in conjunction with antidiabetic oral drugs that allows the
interaction. This study aims to determine the effect of Nattokinase against their power
Metformin HCl in decreasing of blood glucose levels male white rats (Rattus
norvegicus) Wistar. This study uses an oral glucose tolerance test with glucose loading
dose of 4.5 g/kg. Animals test used were white male Wistar rats aged 2-3 months
weighing 150-200 g, 20 rats were divided into 4 groups, each group consisted of 5 rats.
Group I as a negative control group was given tween 80 - span 80, group II as a positive
control group was given metformin HCl doses of 45 mg/kg, group III was given
Nattokinase dose 300 mg/kgBB, group IV given nattokinase 300 mg/kgBB 1 hour then
were given metformin HCl. 30 minutes later all treatment of groups were given glucose.
When treatment is considered as a time at-0. The given of glucose is considered as the
time at-30. Blood sampling via the tail at 0, 30, 60, 120, and 180. Blood glucose levels
was measured with the Easy Touch. The effect of decreasing blood glucose levels
indicated by the value of LDDK0-180 (Regional Area Under the Curve minute 0 to
minute 180 of the graph time vs blood glucose levels). The data can be tested
statistically by Kruskal-Wallis and Mann Whitney with a level of 95%. The results
showed that administration of Metformin HCl after 1 hour administration Nattokinase
can be decreased 52.22% of Metformin HCl.

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Published

2013-05-01

Issue

Vol. 3 No. 1 (2013): Pharmaciana

Section

Analytical Pharmacy and Medicinal Chemistry

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