EFEK FRAKSI ETIL ASETAT EKSTRAK ETANOL AKAR PASAK BUMI (Eurycoma longifolia, Jack) TERHADAP AKTIVITAS FAGOSITOSIS MAKROFAG SECARA IN VITRO

Authors

  • Aminda Nur Arifah Farmasi UAD
  • Nurkhasanah Nurkhasanah Farmasi UAD

DOI:

https://doi.org/10.12928/pharmaciana.v4i1.392

Keywords:

ethyl acetate fraction, Pasak bumi root, macrophage phagocytic activity, immunomodulator

Abstract

Eurycoma longifolia has been used as a remedy for detoxification, aphrodisiaca, anticancer and
immunistimulant. The quassinoids of pasak bumi root extract, have antioxidant activity and
immunomodulatory activity by increasing IL-12. This study aims to determine the effect of the ethyl
acetate fraction of ethanolic extract of pasak bumi root on macrophages phagocytic activity in vitro.
Macrophages were isolated from peritoneal fluid of male Balb/Cmice, and thenwere cultured for 24
hours in 24 well plate. The ethyl actetate fraction ethanolic extract of pasak bumi root with
concentration of 10, 50, and 100 µg/ml, lipopolysaccharides and dimethyl sulfoxide were added, and
incubated for 1.5 hours. The phagocytosis tests was carried out by adding latex with density
5x104
/well and incubated for 1.5 hours at incubator (5% CO2,37o
C). Macrophage were then stained by
Giemsa. The results showed that the percentage of active phagocytic cells treated with ethyl actetate
fraction of ethanolic extract of pasak bumi root of 10, 50 and 100 µg/ml, lipopolysaccharides, and
dimethyl sulfoxide were 95.4%, 90%, 85.4%, 83.4%, and 79% respectively. Phagocytic capacity at
treatment levels of 10, 50, and 100 µg/ml, lipopolysaccharides,and dimethyl sulfoxide were 469; 439.8; 360.8; 204.6; and 147.6 respectively. The result showed thatreatment of ethyl acetate fraction
of ethanolic extract of pasak bumi root can increase the phagocytic activity of macrophages in vitro.

Downloads

Published

2014-05-01

Issue

Vol. 4 No. 1 (2014): Pharmaciana

Section

Analytical Pharmacy and Medicinal Chemistry

License

Authors who publish with Pharmaciana agree to the following terms:

  1. Authors retain copyright and grant the journal the right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA 4.0) that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal. 
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.