Phytochemical constituent, α-amylase and α-glucosidase inhibitory activities of Black Soybean (Glycine soja (L.) Merr.) ethanol extract
DOI:
https://doi.org/10.12928/pharmaciana.v14i3.29919Abstract
Diabetes is characterized as a hyperglycemic condition impacted by β-cell dysfunction and insulin deficiency. Black soybean (Glycine soja (L.) Merr.) is widely known as an origin of nutritious food that has shown activities in preventing cardiovascular disease and reducing hyperglycemia. This research aimed to evaluate the potential of black soybeans ethanol extract (BSEE) as an α-amylase and α-glucosidase activity inhibitor. Black soybean seeds were extracted using the Soxhlet method with 50% ethanol as a solvent. The extract Soybean seeds were screened for the presence of phytochemicals. Inhibitory activity of α-amylase and α-glucosidase enzymes was tested in vitro with acarbose as a control. The absorbance measurement was conducted at 565 nm and 400 nm, respectively. BSEE contained alkaloids, flavonoids, polyphenols, saponins, quinones, tannins, and terpenoids. The results indicated that BSEE exhibited a weak inhibitory effect of α-amylase enzyme activity, with an IC50 value of 360.37 ± 20.80 µg/ml, in contrast to acarbose, which showed a significantly lower IC50 of 4.02 ± 0.56 µg/ml. Meanwhile, BSEE was classified as an active inhibitor of α-glucosidase enzyme activity, presenting 25.67 ± 0.27 µg/mL IC50 value, while acarbose demonstrated 10.85 ± 0.5 µg/mL IC50 value. In conclusion, BSEE inhibits α-amylase and α-glucosidase.
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Hidayat, M., Soeng, S., Prahastuti, S., Erawijantari, P. P., & Widowati, W. (2015). Inhibitory potential of ethanol extract of Detam 1 soybean (Glycine max) seed and Jati belanda (Guazuma ulmifolia) leaves on adipogenesis and obesity models in 3T3-L1 cell line. Journal of Scientific Research & Reports, 6(4), 304–312. https://doi.org/10.9734/JSRR/2015/16273
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Marjoni, & Zulfisa. (2017). Antioxidant activity of methanol extract/fractions of Senggani leaves (Melastoma candidum D. Don). Pharmaceutica Analytica Acta, 08(08), 1–6. https://doi.org/10.4172/2153-2435.1000557
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Prahastuti, S, Hidayat, M., Hasianna, S. T., Widowati, W., Amalia, A., Yusepany, D. T., Rizal, R., & Kusuma, H. S. W. (2019). Antioxidant potential ethanolic extract of Glycine max (l.) Merr. Var. Detam and daidzein. Journal of Physics: Conference Series, 1374(1), 012020. https://doi.org/10.1088/1742-6596/1374/1/012020
Prahastuti, Sijani, Hidayat, M., Kurniadi, M. W., & Christiany, S. (2016). Potency of Black Soybean (Glycine max L. Merr) and Jati Belanda Leaves (Guazuma ulmifolia Lamk) for Dyslipidemia Treatment In Vivo. Journal Of Medicine & Health, 1(3). https://doi.org/10.28932/jmh.v1i3.515
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