Combination of polyherbal Phyllanthus reticulatus with Zingiber officinale and Cymbopogon citratus to optimize the antioxidant capacity
DOI:
https://doi.org/10.12928/pharmaciana.v14i1.28002Keywords:
antioxidant capacity, polyherbal formulation, Zingiber officinale, Cymbopogon citratus, Phyllanthus reticulatusAbstract
Currently, the public is interested in polyherbal-based foods and beverages as a source of natural antioxidants. The aim of the study is to evaluate the antioxidant properties and the phenolic and flavonoid compounds of formulations containing Z. officinale, C. citratus, and P. reticulatus (ZCP). Each sample was extracted using the maceration process in an ethanol solvent at room temperature for three 72-hour periods. There were fourteenth formulation of Z. officinale rhizome, C. citratus leaves, and P. reticulatus fruit which used Design of Expert (DoE). The DPPH method was used to determine the power of antioxidants. The flavonoid content of the extract was measured using the colorimetric method and AlCl3 reagent, while phenolics content using Folin-Ciocalteu. The formulations ZCP 1:0:0, 0:0:1, and 1:1:1 showed the antioxidant capacity in a strong categorization, with an IC50 value less than 50 µg/ml, while ZCP 0:1:0 was in a weak categorization (IC50 > 250 µg/ml). Another ZCP formulation was in a medium category. The ZCP 1:1:1 formulation was suggested as the best one for this investigation, which contains three plant samples. This formulation is interesting for further toxicity studies and in vivo testing so that it can be applied as an antioxidant-rich supplement product.
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Baiano, A., & Nobile, M. A. Del. (2016). Antioxidant compounds from vegetable matrices: biosynthesis, occurrence, and extraction systems. Critical Reviews in Food Science and Nutrition, 56(12), 2053–2068. https://doi.org/https://doi.org/10.1080/10408398.2013.812059
Begum, T., Rahman, M. S., & Rashid, M. A. (2006). Phytochemical and Biological Investigations of Phyllant hus ret iculat us. 5, 21–23.
Bouayed, J., & Bohn, T. (2010). Exogenous antioxidants - Double-edged swords in cellular redox state: Health beneficial effects at physiologic doses versus deleterious effects at high doses. Oxidative Medicine and Cellular Longevity, 3(4), 228–237. https://doi.org/10.4161/oxim.3.4.12858
Foghis, M., Bungau, S. G., Bungau, A. F., Vesa, C. M., Purza, A. L., Tarce, A. G., Tit, D. M., Pallag, A., Behl, T., ul Hassan, S. S., & Radu, A. F. (2023). Plants-based medicine implication in the evolution of chronic liver diseases. Biomedicine and Pharmacotherapy, 158(November 2022), 114207. https://doi.org/10.1016/j.biopha.2022.114207
Irfan, S., Ranjha, M. M. A. N., Nadeem, M., Safdar, M. N., Jabbar, S., Mahmood, S., Murtaza, M. A., Ameer, K., & Ibrahim, S. A. (2022). Antioxidant activity and phenolic content of sonication- and maceration-assisted ethanol and acetone extracts of Cymbopogon citratus leaves. Separations, 9(9). https://doi.org/10.3390/separations9090244
Jamal, a K., Yaacob, W. a, & Din, L. B. (2008). A chemical study on Phyllanthus reticulatus. Journal of Physical Science, 19(2), 45–50.
Mukherjee, S., & Karati, D. (2023). Exploring the phytochemistry, pharmacognostic properties, and pharmacological activities of medically important plant Momordica Charantia. Pharmacological Research - Modern Chinese Medicine, 6(November 2022). https://doi.org/10.1016/j.prmcm.2023.100226
Sindhi, V., Gupta, V., Sharma, K., Bhatnagar, S., Kumari, R., & Dhaka, N. (2013). Potential applications of antioxidants – A review. Journal of Pharmacy Research, 7(9), 828–835. https://doi.org/10.1016/j.jopr.2013.10.001
Wafa, N., Sofiane, G., & Mouhamed, K. (2016). The antioxidant and antimicrobial activities of flavonoids and tannins extracted from Phlomis bovei De Noé. Pelagia Research Library European Journal of Experimental Biology, 6(3), 55–61. www.pelagiaresearchlibrary.com
Wang, X., Chen, J., Jiao, R., Wang, L., Li, Y. M., Zuo, Y., Liu, YuweiPeng, C., Lei, L., Ma, K. Y., Huang, Y., & Chen, Z. Y. (2014). Biology of ageing and role of dietary antioxidants. BioMed Research International, 2014. https://doi.org/10.1155/2014/831841
Xu, D. P., Li, Y., Meng, X., Zhou, T., Zhou, Y., Zheng, J., Zhang, J. J., & Li, H. Bin. (2017). Natural antioxidants in foods and medicinal plants: extraction, assessment and resources. International Journal of Molecular Sciences, 18(1), 20–31. https://doi.org/10.3390/ijms18010096
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