Gastroprotective activity of Banana peel (Musa paradisiaca var. sapientum) methanol extract purified on aspirin-induced gastric ulceration in Rats
DOI:
https://doi.org/10.12928/pharmaciana.v14i3.27639Keywords:
Musa paradisiaca var. sapientum peel, Gastric ulcer, Purified extractAbstract
Banana (Musa paradiasiaca var. sapientum) is the world's most popular fruit-bearing crop, with rising consumption and waste. This study aimed to measure the metabolite compound and evaluate the gastroprotective properties of a banana peel-purified methanol extract. Animals test used in this study were divided into six groups: Group One received NaCMC 0.5%, Group Two received sucralfate, Group Three received aspirin 1000 mg/kg body weight, and groups four, five, and six received PBP at doses of 200 mg/kg body weight, 400 mg/kg body weight, and 600 mg/kg body weight, respectively, for seven days. Except for group 1, all groups were induced with aspirin at 1000 mg/kg body weight on the eighth day. The result of this study exhibited banana peel containing total phenolic, flavonoid, and tannin compounds with concentrations of 33.45 mg GAE/g, 19.92 mg QE/g, and 0.16 %, respectively. The results showed that pure extract of Musa paradiasiaca var. sapientum fruit peel can reduce the incidence of gastric ulcers by decreasing the ulcer index (p<0.05).. The results suggested that Musa paradisiaca var. sapientum peel has a gastroprotective effect against aspirin-induced gastric ulceration.
References
Abdullah, F. C., Rahimi, L., Zakaria, Z. A., & Ibrahim, A. L. (2014). Hepatoprotective, antiulcerogenic, cytotoxic and antioxidant activities of Musa acuminata peel and pulp. Novel Plant Bioresources: Applications in Food, Medicine and Cosmetics, 371-382.
https://doi.org/10.1002/9781118460566.ch26
Ahmed, O. E. I., Hashim, N. M., Yousif, M., Ibrahim, A. A. A., & Ismail Adam, H. A. (2016). Ahmed, O. E. I., Hashim, N. M., Yousif, M., Ibrahim, A. A. A., & Ismail Adam, H. A. (2016). Gastroprotective effects of (+)-catechin hydrate on ethanol-induced gastric ulcer in rats. Cienc Tec Vitivinic, 31. Cienc Tec Vitivinic, 31.
Al Amri, F. S., & Hossain, M. A. (2018). Comparison of total phenols, flavonoids and antioxidant potential of local and imported ripe bananas. Egyptian Journal of Basic and Applied Sciences, 5(4), 245–251. https://doi.org/ 10.1016/j.ejbas.2018.09.002
Awaad, A. S., El-meligy, R. M., & Soliman, G. A. (2013). Natural products in treatment of ulcerative colitis and peptic ulcer. Journal of Saudi Chemical Society, 17(1), 101–124. https://doi.org/10.1016/j.jscs.2012.03.002
Aziakpono, O. M., Ofili, C. C., Chisom, M. U., Johnson, U. A., John, O. A., Sylvanus, M. C., Ogbonnaya, M., Chizoba, N. G., & Harrison, O. U. (2021). Anti-Ulcer Activities of Methanolic Extract of Musa Paradisiaca. Journal of Advances in Medical and Pharmaceutical Sciences, 23(6), 10–17. https://doi.org. 10.9734/jamps/2021/v23i630240
Baba, S. A., & Malik, S. A. (2015). Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii Blume. Journal of Taibah University for Science, 9(4), 449–454. https://doi.org/10.1016/j.jtusci.2014.11.001
Benchikh, F. (2018). Pharmacological effects of Myrtus communis L. on the gastrointestinal tract of rats and mice.Thesis. Université Ferhat Abbas Sétif 1.
Berawi, K. N., & Bimandama, M. A. (2018). The effect of giving extract etanol of kepok banana peel (Musa Acuminata) toward total cholesterol level on male mice (Mus Musculus L.) strain deutschland-denken-yoken (ddy) Obese. Biomedical & Pharmacology Journal, 11(2). https://doi.org/10.13005/bpj/1431
Carleton, H. M., Drury, R. A. B., & Wallington, E. A. (1980). Carleton’s histological technique. Oxford University Press, USA.
Chiu, H.-F., Venkatakrishnan, K.: K, Golovinskaia, O., & Wang, C.-K. (2021). In Vitro and In Vivo Study of Gastroprotective Effect of Purified Exract Andrographis Paniculata. Molecules, 26(7), 2090. https://doi.org/10.3390/molecules26072090
Cho, C. H., & Ogle, C. W. (1979). Cholinergic-mediated gastric mast cell degranulation with subsequent histamine H1-and H2-receptor activation in stress ulceration in rats. European Journal of Pharmacology, 55(1), 23–33. https://doi.org/10.1016/0014-2999(79)90144-4
Cryer, B., & Mahaffey, K. W. (2014). Gastrointestinal ulcers, role of aspirin, and clinical outcomes: pathobiology, diagnosis, and treatment. Journal of Multidisciplinary Healthcare, 7, 137. https://doi.org/10.2147/JMDH.S54324
Dipiro, J., Yee, G., Haines, S., Nolin, T., & Ellingrod, V. (2020). Pharmacotherapy: a pathophysiologic approach. 11th Editi. New york: McGraw-Hill Companies.
Ezekwesili, C. N., Ghasi, S., Adindu, C. S., & Mefoh, N. C. (2014). Evaluation of the anti-ulcer property of aqueous extract of unripe Musa paradisiaca Linn. peel in Wistar rats. African Journal of Pharmacy and Pharmacology, 8(39), 1006–1011.
Farzaei, M. H., Abdollahi, M., & Rahimi, R. (2015). Role of dietary polyphenols in the management of peptic ulcer. World Journal of Gastroenterology: WJG, 21(21), 6499. https://doi.org/10.3748/wjg.v21.i21.6499
Fatimah, C, A., Rahimi, L., Zakaria, Z. A., & Ibrahim, A. L. (2014). Hepatoprotective, antiulcerogenic, cytotoxic and antioxidant activities of Musa acuminata peel and pulp. Novel Plant Bioresources: Applications in Food, Medicine and Cosmetics, 371–382. https://doi.org/10.1002/9781118460566.ch26
Fayyaz, A., Samad, A., Waseem, U., Shafique, S., Nasreen, S., & Hassan, S. (2021). Gastric mucosal damage with aspirin: results of experimental models in adult albino rats. International Journal of Community Medicine and Public Health, 8(1), 50. https://doi.org/10.18203/2394-6040.ijcmph20205447
Gogola, D. (2020). Phytochemical screening, antioxidant and gastro-protective activity studies on the fruit peels of selected varieties of Banana. Herbal Medicines Journal (Herb Med J), 5(2), 45–59. https://doi.org/10.22087/hmj.v5i2.763
Gohar, A. A., & Zaki, A. A. (2014). Assessment of some herbal drugs for prophylaxis of peptic ulcer. Iranian Journal of Pharmaceutical Research: IJPR, 13(3), 1081.
Handa, O., Naito, Y., Fukui, A., Omatsu, T., & Yoshikawa, T. (2014). The impact of non-steroidal anti-inflammatory drugs on the small intestinal epithelium. Journal of Clinical Biochemistry and Nutrition, 54(1), 2–6. https://doi.org/10.3164/jcbn.13-84
Harborne, J. B. (1989). General procedures and measurement of total phenolics. Methods in Plant Biochemistry, 1, 1–28. https://doi.org/10.1016/B978-0-12-461011-8.50007-X
Hervé, E. E., Bernard, G. N., Léandre, K. K., Paul, Y. A., & Etienne, E. E. (2018). Acute toxicity and gastric anti-ulcer activity of an aqueous extract of the leaves of Macaranga barteri Mll. Arg (Euphorbiaceae) on rat models. Journal of Medicinal Plants Research, 12(9), 96–105. https:doi.org/10.5897/JMPR2017.6547
Hossain, M. D., Sarwar, M. S., Dewan, S. M. R., Hossain, M. S., Shahid-Ud-Daula, A. F. M., & Islam, M. S. (2014). Investigation of total phenolic content and antioxidant activities of Azadirachta indica roots. Avicenna Journal of Phytomedicine, 4(2), 97.
Ibrahim, M. F. G., & Allam, F. A. F. A. (2022). Potential stem cell—Conditioned medium and their derived exosomes versus omeprazole in treatment of experimental model of gastric ulcer. Acta Histochemica, 124(4), 151896. https://doi.org/10.1016/j.acthis.2022.151896
IM, F., Birnin-Yauri, A. U., & Jemaima, J. (2014). In vitro antioxidant properties of musa paradisiacal peel aqueous extract. Journal of Scientific and Innovative Research, 3(6), 563–568. https:// doi.org/10.31254/jsir.2014.3603
Jabbar, A. A. (2022). Gastroprotective and Immuno-supportive Role of Alcea kurdica against Stress Induced Lesion in Japanese Quails. Baghdad Science Journal, 19 (4),716-724. https:// doi.org/10.21123/bsj.2022.19.4.0716
Jami’ah, S. R., Ifaya, M., Pusmarani, J., & Nurhikma, E. (2018). Uji aktivitas antioksidan ekstrak metanol kulit pisang raja (Musa paradisiaca sapientum) dengan metode DPPH (2, 2-difenil-1-pikrilhidrazil). Jurnal Mandala Pharmacon Indonesia, 4(1), 33–38. https://doi.org/10.35311/jmpi.v4i1.22
Jang, E., Park, M., Jeong, J. E., Lee, J. Y., & Kim, M. G. (2022). Frequently reported adverse events of rebamipide compared to other drugs for peptic ulcer and gastroesophageal reflux disease. Scientific Reports, 12(1), 7839. https://doi.org/10.1038/s41598-022-11505-0
Kadhem, M. A., Abdul-Niby, A. A., & Khassaf, H. K. (2018). Study the effect of ethanolic extract of Anethum graveolens L. on aspirin induced Gastric Ulcer in Male Guinea Pigs. Research Journal of Pharmacy and Technology, 11(9), 3793–3798. https://doi.org/10.5958/0974-360X.2018.00695.9
Kapadia, S. P., Pudakalkatti, P. S., & Shivanaikar, S. (2015). Detection of antimicrobial activity of banana peel (Musa paradisiaca L.) on Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans: An in vitro study. Contemporary Clinical Dentistry, 6(4), 496. https://doi.org/10.4103/0976-237X.169864
Kathirvelu, P., Cheriyan, B. V., Jagan, N., Viswanathan, S., Kumar, S. V., & Kamalakannan, P. (2019). Gastroprotective and Antioxidant effect of Petroleum ether Extract of Eupatorium triplinerve Vahl. Research Journal of Pharmacy and Technology, 12(11), 5163–5166. https://doi.org/10.5958/0974-360X.2019.00893.X
Kibria, A. A., Kamrunnessa, Rahman, M. M., & Kar, A. (2019). Extraction and Evaluation of Phytochemicals from Banana Peels ( Musa sapientum ) and Banana Plants ( Musa paradisiaca ) . Malaysian Journal of Halal Research, 2(1), 22–26. https://doi.org/10.2478/mjhr-2019-0005
Kumar, M., Gautam, M. K., Singh, A., & Goel, R. K. (2013). Healing effects of Musa sapientum var. paradisiaca in diabetic rats with co-occurring gastric ulcer: cytokines and growth factor by PCR amplification. BMC Complementary and Alternative Medicine, 13(1), 1–9. https://doi.org/10.1186/1472-6882-13-305
Kumar, S., & Pandey, A. K. (2013). Chemistry and biological activities of flavonoids: an overview. The Scientific World Journal, 2013. https://doi.org/10.1155/2013/162750
Kuna, L., Jakab, J., Smolic, R., Raguz-Lucic, N., Vcev, A., & Smolic, M. (2019). Peptic ulcer disease: a brief review of conventional therapy and herbal treatment options. Journal of Clinical Medicine, 8(2), 179. https://doi.org/10.3390/jcm8020179
Lambert, A. A., Lam, J. O., Paik, J. J., Ugarte-Gil, C., Drummond, M. B., & Crowell, T. A. (2015). Risk of community-acquired pneumonia with outpatient proton-pump inhibitor therapy: a systematic review and meta-analysis. PloS One, 10(6), e0128004. https://doi.org/10.1371/journal.pone.0128004
Lanas, A., & Chan, F. K. L. (2017). Peptic ulcer disease. The Lancet, 390(10094), 613–624. https://doi.org/10.1016/S0140-6736(16)32404-7
Lewis, D. A., Fields, W. N., & Shaw, G. P. (1999). A natural flavonoid present in unripe plantain banana pulp (Musa sapientum L. var. paradisiaca) protects the gastric mucosa from aspirin-induced erosions. Journal of Ethnopharmacology, 65(3), 283–288. https://doi.org/10.1016/S0378-8741(99)00005-7
Lewis, D. A., & Shaw, G. P. (2001). A natural flavonoid and synthetic analogues protect the gastric mucosa from aspirin-induced erosions. The Journal of Nutritional Biochemistry, 12(2), 95–100. https://doi.org/10.1016/S0955-2863(00)00133-9
Li, C., Wang, L., Zhao, J., Wei, Y., Zhai, S., Tan, M., Guan, K., Huang, Z., & Chen, C. (2022). Lonicera rupicola Hook. f. et Thoms flavonoids ameliorated dysregulated inflammatory responses, intestinal barrier, and gut microbiome in ulcerative colitis via PI3K/AKT pathway. Phytomedicine, 104, 154284. https://doi.org/10.1016/j.phymed.2022.154284
Mahurkar, N., & Sayeed Ul Hasan, S. M. (2015). Synergistic Antiulcer Effect of Melatonin and Esomeprazole Combination in Pylorus Ligation, Ethanol, Aspirin induced Peptic Ulcers. Asian Journal of Pharmaceutical Research, 5(1), 10–14. https://doi.org/10.5958/2231-5691.2015.00002.7
Malik, T. F., Gnanapandithan, K., & Singh, K. (2018). Peptic ulcer disease.
Mohammed, M. A., El-Hadi, A. E., Mohammed, E. A., Abbas, F. S., Musa, K. O., Al-Ameen, M. Y., Ahmed, A. M., Gassab, M. T. I., Hassan, H. M., & Abdelgadir, A. A. (2021). Gastro-protective Effects of Green Banana (Musa cavendishii Lamb.) Pulp Powder on Aspirin-induced Gastric Ulcer in Albino Rats.
Morais, D. R., Rotta, E. M., Sargi, S. C., Schmidt, E. M., Bonafe, E. G., Eberlin, M. N., Sawaya, A. C. H. F., & Visentainer, J. V. (2015). Antioxidant activity, phenolics and UPLC–ESI (–)–MS of extracts from different tropical fruits parts and processed peels. Food Research International, 77, 392–399. https://doi.org/10.1016/j.foodres.2015.08.036
Narayanan, M., Reddy, K. M., & Marsicano, E. (2018). Peptic ulcer disease and Helicobacter pylori infection. Missouri Medicine, 115(3), 219.
Obioma, N. E., Kelechi, O. J., Miracle, N. T., Ogbonnaya, E., & Offor, N. (2018). Ulcer healing effect of ethanolic extract of unripe musaparadisiaca on aspirin induced gastric ulceration in adult wistar rats. European Journal of Pharmaceutical and Medical Research, 5(12), 58–62.
Onasanwo, S. A., Emikpe, B. O., Ajah, A. A., & Elufioye, T. O. (2013). Anti-ulcer and ulcer healing potentials of Musa sapientum peel extract in the laboratory rodents. Pharmacognosy Research, 5(3), 173–178. https://doi.org/10.4103/0974-8490.112423
Palle, S., Kanakalatha, A., & Kavitha, C. N. (2018). Gastroprotective and antiulcer effects of Celastrus paniculatus seed oil against several gastric ulcer models in rats. Journal of Dietary Supplements, 15(4), 373–385. https://doi.org/10.1080/19390211.2017.1349231
Pereira, A., & Maraschin, M. (2015). Banana (Musa spp) from peel to pulp: ethnopharmacology, source of bioactive compounds and its relevance for human health. Journal of Ethnopharmacology, 160, 149–163. https://doi.org/10.1016/j.jep.2014.11.008
Pulipati, S., Babu, P. S., & Narasu, M. L. (2014). Quantitative determination of tannin content and evaluation of antibacterial activity of Amaranthus tricolor (L). Int J Biol Pharm Res, 5, 623–626.
Puri, B., & Hall, A. (1998). Phytochemical dictionary: a handbook of bioactive compounds from plants. CRC press.
Pusmarani, J., Ifaya, M., Isrul, M., & Saleh, A. (2019). Gastroprotective effect of Lannea Coromandelica Stem Bark in Rat induced by aspirin. Research Journal of Pharmacy and Technology, 12(4), 1646–1648. https://doi.org/10.5958/0974-360X.2019.00275.0
Pusmarani, J., Ifaya, M., & Putri, R. J. (2022). Hepatoprotector Effect of Banana Peel (Musa paradisiaca Sapientum) on Paracetamol Induced Rats. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy)(e-Journal), 8(2), 109–116. https://doi.org/10.22487/j24428744.2022.v8.i2.15968
Pusmarani, J., Putri, R. J., Dewi, C., Purwono, S., & Ikawati, Z. (2019). Non Specific and Specific Parameter Standardization Of Banana Peel (Musa paradisciata Sapientum) and Andrographis Paniculata. International Summit on Science Technology and Humanity, 658–664.
Rahman, Z., Dwivedi, D. K., & Jena, G. B. (2020). Ethanol-induced gastric ulcer in rats and intervention of tert-butylhydroquinone: involvement of Nrf2/HO-1 signaling pathway. Human & Experimental Toxicology, 39(4), 547–562. https://doi.org/10.1177/0960327119895559
Raish, M., Shahid, M., Bin Jardan, Y. A., Ansari, M. A., Alkharfy, K. M., Ahad, A., Abdelrahman, I. A., Ahmad, A., & Al-Jenoobi, F. I. (2021). Gastroprotective effect of sinapic acid on ethanol-induced gastric ulcers in rats: involvement of Nrf2/HO-1 and NF-κB signaling and antiapoptotic role. Frontiers in Pharmacology, 12, 622815. https://doi.org/10.3389/fphar.2021.622815
Rita, S. W., Swantara, I. M. D., Asih, I., & Puspawati, N. M. (2020). Antibacterial activity and antioxidant capacity of selected local banana peel (Musa sp.) methanol extracts cultivated in Bali. Int. J. Agric. Environ. Biores, 5, 242–251. https://doi.org/10.35410/IJAEB.2020.5519
Saheed, S., Olarewaju, S. A., Taofeeq, G., Olatunde, S. T., & Alanamu, A. A. (2015). Combined administration of Spondias mombin and Ficus exasperata leaf extracts stall Indomethacin-mediated gastric mucosal onslaught in rats. African Journal of Traditional, Complementary and Alternative Medicines, 12(1), 45–51. https://doi.org/10.4314/ajtcam.v12i1.7
Saiah, W., Halzoune, H., Djaziri, R., Tabani, K., Koceir, E. A., & Omari, N. (2018). Antioxidant and gastroprotective actions of butanol fraction of Zingiber officinale against diclofenac sodium‐induced gastric damage in rats. Journal of Food Biochemistry, 42(1), e12456. https://doi.org/10.1111/jfbc.12456
Sakura, Y. W., Jayawardhita, A. A. G., Kardena, I. M., & Sudimartini, L. M. (2017). Perbandingan gambaran histopatologi lambung tikus putih (Rattus norvegicus) jantan yang diberi amoxicillin dikombinasikan dengan asam mefenamat dan deksametason. Indonesia Medicus Veterinus, 6(3), 246–253.
Shahidi, F., & Ambigaipalan, P. (2015). Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects–A review. Journal of Functional Foods, 18, 820–897. https://doi.org/10.1016/j.jff.2015.06.018
Sharifi-Rad, J., Quispe, C., Zam, W., Kumar, M., Cardoso, S. M., Pereira, O. R., Ademiluyi, A. O., Adeleke, O., Moreira, A. C., & Živković, J. (2021). Phenolic bioactives as antiplatelet aggregation factors: the pivotal ingredients in maintaining cardiovascular health. Oxidative Medicine and Cellular Longevity, 2021. https://doi.org/10.1155/2021/2195902
Singh, B., Singh, J. P., Kaur, A., & Singh, N. (2016). Bioactive compounds in banana and their associated health benefits–A review. Food Chemistry, 206, 1–11. https://doi.org/10.1016/j.foodchem.2016.03.033
Sisay Zewdu, W., & Jemere Aragaw, T. (2020). Evaluation of the anti-ulcer activity of hydromethanolic crude extract and solvent fractions of the root of Rumex nepalensis in Rats. Journal of Experimental Pharmacology, 325–337. https://doi.org/10.2147/JEP.S258586
Thomas, A., & Krishnakumar, K. (2017). Banana peel: pharmacological activities: a Review. International Journal of Innovative Research and Advanced Studies, 4(5), 62–64.
Ulser, T. (2016). Antiulcer activity of Musa paradisiaca [banana] tepal and skin extracts in ulcer induced albino mice. Malaysian Journal of Analytical Sciences, 20(5), 1203–1216. https://doi.org/10.17576/mjas-2016-2005-27
Vu, H. T., Scarlett, C. J., & Vuong, Q. V. (2018). Phenolic compounds within banana peel and their potential uses: A review. Journal of Functional Foods, 40, 238–248. https://doi.org/10.1016/j.jff.2017.11.006
Wang, Z., & Wei, Y. (2022). SEMA3D Plays a critical role in peptic ulcer disease-related Carcinogenesis Induced by H. pylori Infection. International Journal of General Medicine, 15, 1239. https://doi.org/10.2147/IJGM.S343635
Washio, E., Esaki, M., Maehata, Y., Miyazaki, M., Kobayashi, H., Ishikawa, H., Kitazono, T., & Matsumoto, T. (2016). Proton pump inhibitors increase incidence of nonsteroidal anti-inflammatory drug–induced small bowel injury: a randomized, placebo-controlled trial. Clinical Gastroenterology and Hepatology, 14(6), 809-815.e1. https://doi.org/10.1016/j.cgh.2015.10.022
Yaghoobi, M., & Armstrong, D. (2022). Peptic ulcer disease. In Yamada’s Textbook of Gastroenterology (pp. 924–976). Wiley. https://doi.org/10.1002/9781119600206.ch49
Yandrapu, H., & Sarosiek, J. (2015). Protective factors of the gastric and duodenal mucosa: an overview. Current Gastroenterology Reports, 17(6), 24. https://doi.org/10.1007/s11894-015-0452-2
Zaghlool, S. S., Abo-Seif, A. A., Rabeh, M. A., Abdelmohsen, U. R., & Messiha, B. A. S. (2019). Gastro-protective and anti-oxidant potential of Althaea officinalis and solanum nigrum on pyloric ligation/indomethacin-induced ulceration in rats. Antioxidants, 8(11), 512. https://doi.org/10.3390/antiox8110512
Zheng, Y.-F., Xie, J.-H., Xu, Y.-F., Liang, Y.-Z., Mo, Z.-Z., Jiang, W.-W., Chen, X.-Y., Liu, Y.-H., Yu, X.-D., Huang, P., & Su, Z.-R. (2014). Gastroprotective effect and mechanism of patchouli alcohol against ethanol, indomethacin and stress-induced ulcer in rats. Chemico-Biological Interactions, 222, 27–36. https://doi.org/https://doi.org/10.1016/j.cbi.2014.08.008
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