Chlorogenic acid in preventing and curing ultraviolet-induced damage in human skin fibroblast as an antiaging cell model

Authors

  • Ermi Girsang Faculty of Medicine, University of Prima Indonesia, Medan, Indonesia.
  • I Nyoman Ehrich Lister FFaculty of Medicine, University of Prima Indonesia, Medan, Indonesia
  • Chrismis Novilda Ginting Faculty of Medicine, University of Prima Indonesia, Medan, Indonesia.
  • Wahyu Widowati Faculty of Medicine, Maranatha Christian University, Bandung 40164, West Java, Indonesia
  • Seila Arumwardana Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung 40163, West Java, Indonesia
  • Meganita Marthania Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung 40163, West Java, Indonesia
  • Rizal Rizal Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung 40163, West Java, Indonesia Biomedical Engineering Department of Electrical Engineering, Faculty of Engineering Universitas Indonesia, Depok 1642, Indonesia

DOI:

https://doi.org/10.12928/pharmaciana.v13i2.24459

Keywords:

aging, cell viability, chlorogenic acid, fibroblast cell, ultraviolet

Abstract

Continuous ultraviolet (UV) irradiation stimulates the over-production of reactive oxygen species (ROS) to cause degenerative diseases. Chlorogenic acid (CA) is found as plants antioxidant that promises medicinal effects. This study examined CA protection against UV-damage in human skin fibroblast (BJ) cells both for curative and preventive therapy. BJ cells were exposed to UV radiation and the addition of CA (6.26-100 mikro g/mL) by preventive and curative addition methods. The cells viability analysis was conducted employing MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay. CA treatment before UV exposure exhibited an increased percentage of viability cells than the positive control. In detail, the series of CA concentration (6.25, 12.5, and 25 mikro g/mL) significantly enhanced the percentage of viable cells. The addition of CA after UV exposure denoted the same results. Furthermore, the lower CA concentrations used, the higher cell viability resulted. CA at dose 6.25 mikro g/mL showed the highest viability in cells, while CA 100 mikro g/mL resulted in the lowest viability. In short, CA can preserve and treat cells from UV exposure. The outcome suggested prevention and curative on UV-induced BJ cells, and the tested concentration is applicable for further experiments.

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Published

2023-07-26

Issue

Vol. 13 No. 2 (2023): Pharmaciana

Section

Analytical Pharmacy and Medicinal Chemistry

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