Standardization of Cassia spectabilis DC leaves and antimalarial activities of ethyl acetate extract

Authors

  • Wiwied Ekasari Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga http://orcid.org/0000-0003-3163-5829
  • Heny Arwati Department of Parasitology, Faculty of Medicine, Universitas Airlangga
  • Nindya Tresiana Putri Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga
  • Dewi Hariyani Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga
  • Rosalia Friska Ananda Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga
  • Eko Suhartono Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Lambung Mangkurat

DOI:

https://doi.org/10.12928/pharmaciana.v12i2.23598

Keywords:

malaria, Cassia spectabilis, heme detoxification, Plasmodium berghei, Plasmodium falciparum, standardization

Abstract

Cassia spectabilis is one of the Indonesian medicinal plants traditionally used to treat different diseases, including malaria. Quality of a drug derived from plants is also influenced by the quality of its raw materials. Thus, in order to assure the quality of products made from plants, it is necessary to standardize the raw materials and following with the antimalarial activity test. The aim of this study was to standardize the quality of C. spectabilis leaves and to evaluate its antimalarial activities of ethyl acetate extract. The fresh material of C. spectabilis leaves were observed its specific and non-specific parameters. In vitro test was done by using Plasmodium falciparum 3D7. In vivo test was done using 4-day suppressive test method against mice infected with P. berghei for four consecutive days. Heme detoxification inhibitory activity test was carried out using the modified Basilico method. The leaves of C. spectabilis meet the quality requirement for raw material of traditional medicine. The ethyl acetate extract showed in vitro antiplasmodial activity against P. falciparum 3D7 and in vivo antimalarial activity against P. berghei infection with IC50 value of 27.28 μg/mL and ED50 value of 1.74 mg/kg, respectively. The extract also showed heme detoxification inhibitory activity with IC50 value of 0.33±0.01 mg/mL. The leaves of C. spectabilis leaves meet the quality requirement and the ethyl acetate extract from standardized C. spectabilis leaves possessed a potential an antimalarial activity which deserves to be further developed.

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Published

2022-07-04

Issue

Vol. 12 No. 2 (2022): Pharmaciana

Section

Biology Pharmacy

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