Chemical qualitative analysis and spf value stability of nutmeg seed oil in microemulsions with tween 80 and PEG 400 as surfactants and cosurfactants

Authors

  • Ayu Shabrina Universitas Wahid Hasyim
  • Erika Indah Safitri Faculty of Pharmacy, University of Wahid Hasyim, Semarang
  • Risha Fillah Fithria
  • Misbahul Munir Faculty of Pharmacy, University of Wahid Hasyim, Semarang
  • Sumantri Sumantri Faculty of Pharmacy, University of Wahid Hasyim, Semarang

DOI:

https://doi.org/10.12928/pharmaciana.v12i1.21997

Keywords:

nutmeg Oil, , microemulsion, SPF, Stability, GC-MS

Abstract

Nutmeg oil contains α-pinene, which can be used as sunscreen. The combination of Tween 80 and PEG 400 can maintain the stability of nutmeg oil microemulsion. This research was a follow-up study that aims to determine the stability of the SPF value and qualitative chemical content of nutmeg seed oil microemulsions (NSM). NSM was made with a nutmeg seed oil concentration of 6.4% and tween 80 and PEG 400 as surfactants and cosurfactants with variations in the ratio of F1 (5: 4), F2 (6: 4), and F3 (7: 4). Nutmeg seed oil and NSM content was analyzed using GC-MS. NSM formula were tested for in vitro SPF value stability by storing NSM in a climatic chamber at 30 °C ± 2 °C with RH 65 % ± 5 % for 4 weeks. The SPF values were calculated every week. GC-MS data were analyzed descriptively and data of SPF value stability were analyzed statistically using one way ANOVA. The GC-MS results of nutmeg seed oil showed 35 components, including significant compounds, namely α-pinene, sabinene, β-phellandrene and also α-terpinolene. GC-MS results of NSM showed those significant compounds were still detected after being formulated in microemulsion. The results of the sunscreen activity test of NSM before storage were 10.31 ± 0.03 (F1); 10.47 ± 0.07 (F2); 10.45 ± 0.03 (F3) and did not show significant change after storage for 4 weeks (p > 0.05). The SPF values of NSM were categorized in maximum activity.

 

Author Biography

Ayu Shabrina, Universitas Wahid Hasyim

Departemen Farmasetika dan Teknologi Farmasi

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Published

2022-03-10

Issue

Vol. 12 No. 1 (2022): Pharmaciana

Section

Pharmaceutics and Pharmaceutical Technology

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