Incision wound healing activity of free-range chicken (Gallus domesticus) egg white gel in mice

Authors

  • Wahyu Widyaningsih Universitas Ahmad Dahlan
  • Sapto Yuliani Universitas Ahmad Dahlan
  • Ayu Wulandari Universitas Ahmad Dahlan
  • Rifka Salsabila Universitas Ahmad Dahlan

DOI:

https://doi.org/10.12928/pharmaciana.v11i3.20531

Keywords:

wound healing, egg white gel, incision

Abstract

Egg whites reportedly exhibit wound healing activities by forming new cell tissues and accelerating damaged cell tissue recovery. This study aimed to determine whether and how egg whites prepared in gel dosage forms healed incision wounds in mice (Mus musculus). The method began with formulating gels by mixing egg whites with Carbopol 940, methylparaben, propylparaben, propylene glycol, triethanolamine, and distilled water. Sixteen mice were divided evenly into four groups, and each was subjected to a 1 cm long midline dorsal incision. Group I was given 10% povidone-iodine ointment, group II was gel base, group III was 10% egg white gel, and group IV was 20% egg white gel. Based on the test parameters measured during macroscopic observation, namely wound length and healing time, the gel preparations were found to fulfill the physical properties requirements, and incision wounds treated with 10% and 20% egg white gels healed in 9 and 12.5 days, respectively. The statistical test revealed a significant difference in wound size on Day 10 and in healing time between wound treatments with 10% gel and base gel (p < 0.05). Therefore, it can be concluded that the 10% egg white gel is effective in healing cuts in mice as it speeds wound healing and decreases wound size.

References

Danarti, R., Budiyanto, A., & Wirohadidjojo, W. (2014). The effect povidone-iodine on the wound healing process : A study on fibroblast populated collagen lattice ( FPCL ) model. 46(3), 103–107. https://doi.org/10.19106/JMedScie.004603201401

Gabriela, M., Dantas, B., Alan, S., Bomfim, G., Mahara, C., Damasceno, D., Rolim, L. A., Rolim-neto, P. J., Carvalho, F. O., Quintans-junior, L. J., Roberto, J., & Almeida, S. (2016). Development and evaluation of stability of a gel formulation containing the monoterpene borneol. https://doi.org/10.1155/2016/7394685

Garg, A., Aggarwal, D., Garg, S., & Singla, A. (2002). Spreading of semisolid formulations: An update. Pharmaceutical Technology, 26, 84–105.

Hendriati, L., Hamid, I. S., Widodo, T., Wandasari, C., & Risata, P. M. (2018). Effect of egg white gel againts burn healing on white rat (Rattus novergicus). Jurnal Ilmu Kefarmasian Indonesia, 16(2), 231. https://doi.org/10.35814/jifi.v16i2.532

Legros, J., Jan, S., Bonnassie, S., Gautier, M., Croguennec, T., Cochet, M., Nau, F., & Andrews, S. C. (2021). The role of ovotransferrin in egg-white antimicrobial activity : a review. https://doi.org/10.3390/foods10040823

Megawati, Roosevelt, A., & Akhir, L. O. (2019). Formulasi dan uji stabilitas fisiksediaan gel ekstrak kulit buah rambutan (Nephelium lappaceum L.) sebagai obat sariawan menggunakan variasi konsentrasi basis karbopol. 5, 5–10

Nurman, S., Yulia, R., Noor, E., & Sunarti, T. C. (2019). The optimization of gel preparations using the active compounds of arabica coffee ground nanoparticles. https://doi.org/10.3390/scipharm87040032

Percival, S. L. (2014). Wounds and infection. In Biofilms in Infection Prevention and Control. Elsevier Inc. https://doi.org/10.1016/B978-0-12-397043-5.00008-6

Purnani, W. T. (2019). Perbedaan efektivitas pemberian putih telur dan ikan gabus terhadap penyembuhan luka perineum ibu nifas. Journal of Public Health Research and Community Health Development, 2, 139–146. https://doi.org/10.1017/CBO9781107415324.004

Putrianirma, R., Triakoso, N., Yunita, M. N., Yudaniayanti, I. S., Hamid, I. S., & Fikri, F. (2019). Efektivitas ekstrak daun afrika (Vernonia amygdalina) secara topikal untuk reepitelisasi penyembuhan luka insisi pada tikus putih (Rattus novergicus). Jurnal Medik Veteriner, 2(1), 30. https://doi.org/10.20473/jmv.vol2.iss1.2019.30-35

Rahmawati, D. A., & Setiawan, I. (2019). The formulation and physical stability test of gel fruit strawberry extract (Fragaria x ananassa Duch.). 38–46.

Simanjuntak, M. . (2006). Biofarmasi sediaan yang diberikan melalui kulit.Universitas Sumatera Utara

Sorg, J. M. R. H. (2012). Wound repair and regeneration. 35–43. https://doi.org/10.1159/000339613

Suryani, N., Mubarika, D. N., & Komala, I. (2019). Pengembangan dan evaluasi stabilitas formulasi gel yang mengandung etil p -metoksisinamat. Pharmaceutical and Biomedical Sciences Journal, 1(November), 29–36.

Ulaen, S. P., Banne, Y., & Suatan, R. A. (2012). Pembuatan salep anti jerawat dari ekstrak rimpang temulawak (Curcuma xanthorrhiza Roxb.). Jurnal Ilmiah Farmasi Poltekkes Manado, 3(2), 96587.

Utariani, A., Rahardjo, E., & Perdanakusuma, D. S. (2020). Effects of albumin infusion on serum levels of albumin , proinflammatory cytokines ( TNF- α , IL-1 , and IL-6 ), CRP , and MMP-8 ; Tissue Expression of EGRF , ERK1 , ERK2 , TGF- β , Collagen , and MMP-8 ; and wound healing in sprague dawley rats. https://doi.org/10.1155/2020/3254017

Utariani, A., Rahardjo, E., Perdanakusuma, D. S., Rehatta, N. M., Santoso, K. H., & Semedi, B. P. (2021). The comparison effect between albumin infusion and normal protein diet on the acceleration of wound healing : a randomized control trial. 312–315. https://doi.org/10.5220/0008156403120315

Widjaya, H. A. (2016). Efektivitas gel putih telur pada penyembuhan luka insisi tikus putih (Rattus novergicus) melalui pengamatan penyembuhan luka dan sel makrofag. Widya Mandala Catholic University Surabaya.

Yao, K., & Bae, L. (2013). Post-operative wound management. 42(12), 867–870.

Zhang, Y., Wang, D., Smuda, K., Geogieva, R., Baumler, H., & Gao, C. (2018). Inflammatory activation of human serum albumin- or ovalbumin-modified chitosan particles to macrophages and their immune response in human whole blood Yixian. Journal of Materials Chemistry B, 6 (19), 3096–3106. https://doi.org/10.1039/C7TB03096G

Downloads

Published

2021-10-23

Issue

Vol. 11 No. 3 (2021): Pharmaciana

Section

Pharmacology

License

Authors who publish with Pharmaciana agree to the following terms:

  1. Authors retain copyright and grant the journal the right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA 4.0) that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal. 
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.