Antibacterial activity of mexican sunflower leaf Tithonia diversifolia (Hemsl.) A.Gray Aqueous extract against methicillin-resistant Staphylococcus aureus
DOI:
https://doi.org/10.12928/pharmaciana.v12i1.20469Keywords:
Antibacterial, aqueous extract of Mexican sunflower leaves, Methicillin-Resistant Staphylococcus aureus, Tithonia diversifolia (Hemsl.) A. GrayAbstract
The research of antibacterial activity of Mexican sunflower leaf Tithonia diversifolia (Hemsl.) A.Gray aqueous extract against Methicillin-Resistant Staphylococcus aureus (MRSA) was carried out. The research aimed to observe the antibacterial activity of Mexican sunflower leaves aqueous extract to inhibit the growth of MRSA with concentrations of 10, 20, 30, 40, and 50%. The extracts were obtained by the maceration method, and the antibacterial activity was tested using the agar well diffusion method. Characterization of Mexican sunflower leaves simplicia were obtained with water level 9%, water-soluble level 21,6%, ethanol-soluble level 10,3%, and total ash level 14,36%. Characterization of Mexican sunflower leaves aqueous extracts were obtained with water level 26,36%, water-soluble level 53,13%, ethanol-soluble level 26,36%, and total ash level 19,98%. Phytochemical screening revealed that aqueous extract of Mexican sunflower leaves contained secondary metabolites of alkaloids, flavonoids, tannins, and saponins. The largest inhibitory zone was shown at a 50% extract concentration with a diameter of 12,40 mm. The aqueous extract of Mexican sunflower leaves was capable to form the inhibition zone on the MRSA growth.
References
Agidigbi, T. S., & Odeyemi, O. (2014). Antibacterial Activities of Crude Extracts of Tithonia Diversifolia. The Experiment, 20(4), 1421–1426
Boateng, J., & Diunase, K. N. (2015). Comparing the antibacterial and functional properties of cameroonian and manuka honeys for potential wound healing-have we come full cycle in dealing with antibiotic resistance? Molecules, 20(9), 16068–16084
CLSI. (2014). M100-S23 Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement (Issue January)
Depkes RI. (1995). Materia Medika Indonesia Jilid VI. Depkes RI
Depkes RI. (2000). Parameter standar umum ekstrak tumbuhan obat cetakan pertama. Depkes RI.
Dewi, F. K. (2013). Aktivitas antibakteri ekstrak etanol buah Mengkudu (Morinda citrifolia, Linnaeus) terhadap bakteri pembusuk daging segar. Skripsi, 9(1), 76–99
Febrina, L., Rusli, R., & Muflihah, F. (2015). Optimalisasi ekstraksi dan uji metabolit sekunder tumbuhan Libo (Ficus variegate Blume). Journal of Tropical Pharmacy and Chemistry, 3(2), 74–81. https://doi.org/10.25026/jtpc.v3i2.153
Harborne, J. B. (1987). Metode Kimia :Penuntun Modern Menganalisis Tumbuhan. Terjemahan Kosasi Padmawinata & Iwang Soediro. Penerbit ITB
Karou, D., Savadogo, A., Canini, A., Yameogo, S., Montesano, C., Simpore, J., Colizzi, V., & Traore, A. S. (2005). Antibacterial activity of alkaloids from Sida acuta. African Journal of Biotechnology, 4(12), 1452–1457
Kuntaman, K., Hadi, U., Setiawan, F., Koendori, E. B., Rusli, M., Santosaningsih, D., Saverin, J., & Verbrugh, H. A. (2016). Prevalence of methicillin resistant staphylococcus aureus from nose and throat of patients on admission to medical wards of Dr Soetomo Hospital, Surabaya, Indonesia. The Southeast Asian Journal of Tropical Medicine and Public Health, 47(1), 66–70
Liasu, M. O. and Ayandele, A. A. (2008). Antimicrobial activity of aqueous and ethanolic extracts from Tithonia diversifolia and Bryum coronatum collected from Ogbomoso, OyoState, Nigeria. Advances in Natural and Applied Sciences, 2(1), 31-34
Madduluri, S., Babu Rao, K., & Sitaram, B. (2013). In vitro evaluation of antibacterial activity of five indigenous plants extract against five bacterial pathogens of human. International Journal of Pharmacy and Pharmaceutical Sciences, 5(SUPPL.4), 679–684
Ningsih, D. R., Zusfahair, & Kartika, D. (2016). Identifikasi senyawa metabolit sekunder serta uji aktivitas ekstrak daun sirsak sebagai antibakteri. Molekul,11(1), 101–111
Prasetyo, & Inoriah, E. (2013). Pengelolaan budidaya tanaman obat-obatan bahan simplisia. Badan Penerbitan Fakultas Pertanian UNIB
Tokajian, S. (2014). New epidemiology of Staphylococcus aureus infections in the Middle East. Clinical Microbiology and Infection, 20(7), 624–628. https://doi.org/10.1111/1469-0691.12691
WHO. (2003). Manual for the laboratory identification and antimicrobial susceptibility testing of bacterial pathogens of public health importance in the developing world. In World Health Organization (WHO)
WHO. (2011). Quality control methods for herbal materials
Zurniati, A. Z. (2011). Pengaruh pemberian ekstrak air daun Kembang Bulan (Tithonia diversifolia) terhadap Pertumbuhan Staphylococcus aureus dan Escherichia coli. Skripsi
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