Isolation and identification of secondary metabolites in ethyl acetate extract from the Maja bark (Aegle marmelos Linn.)

Authors

  • Muhammad Syahrir Universitas Negeri Makassar
  • Eka Bungin Kadola Universitas Negeri Makassar
  • Pince Salempa Universitas Negeri Makassar

DOI:

https://doi.org/10.12928/pharmaciana.v11i1.17875

Keywords:

Aegle marmelos (Linn.), alkaloids and secondary metabolites

Abstract

This exploratory study resulted in the isolation and identification of secondary metabolites in ethyl acetate extracts from the Maja bark (Aegle marmelos Linn.) from Pattappa Village, Pujananting Subregency, Barru Regency, South Sulawesi. The stages of isolation included maceration, fractionation, and purification. Maceration was performed with methanol, fractionation with ethyl acetate, a particular ratio of acetone (9:1) was used, and purification was done by flowing nitrogen gaseous into the E5 fraction. Furthermore, the classification test and FTIR spectrophotometer were carried out for identification. Pure isolates in the form of white crystals were obtained with a melting point of 242oC. Reagent tests indicated a brown precipitate on Wagner's test and a white precipitate on Mayer's test. Infrared-spectrophotometric identification provided typical absorption for such functional groups as NH, aliphatic CH, aromatic C=C, and amine CN.  The reagent tests and FTIR-spectrophotometer identification confirmed that the secondary metabolites fit well into the class of alkaloids.

Author Biographies

Muhammad Syahrir, Universitas Negeri Makassar

Departmen of Chemistry FMIPA UNM

Pince Salempa, Universitas Negeri Makassar

Chemistry Department FMIPA UNM

References

Achmad, S. A., Hakim, E. H., Erwin, S. M., Nario, A., Mariko, K., Lukman, M., & Hiromitsu, T. (2001). Artoindonesianin B suatu senyawa yang bersifat toksik terhadap sel tumor P-388 dari tumbuhan Artocarpus altilis. Bulletin The Indonesian Society of Natural Product Chemistry, 20–27

Atun, S. (2010). Pemanfaatan bahan alam menuju riset internasional. 1–15

Firdaus. (2011). Teknik dalam laboratorium kimia organik (p. 108). Prodi Kimia Jurusan Kimia FMIPA Universitas Hasanuddin

Gupta, V. K., Goswami, S., & B.D.Gupta. (2006). Crystal structure of R-(+)-marmin. Analytical Sciences, 22, X11–X12

Harborne J.B. (1987). Metode Fitokimia. Insitut Teknologi Bandung

Ibrahim, H. I., & Rusdiaman. (2017). Identifikasi senyawa alkaloid daging buah maja (Aegle marmelos) asal batu bessi kabupaten barru Sulawesi Selatan. Media Farmasi, XIII(1), 105–110

Iwan D, & Darminto. (2012). Kimia organik bahan alam: metode isolasi dan identifikasi senyawa kimia organik metabolit sekunder Dari Bahan Alam. Jurusan Kimia FMIPA Universitas Negeri Makassar

Mirsha, B. B., Navneet Kishore, Vinod K.Tiwari, D.Singh, D., & Tripathi, V. (2010). A novel anti fungal anthraquinone from seeds of Aegle marmelos correa (Family rutaceae). Fitoterapia, 81, 104–107

Mohrig, J. R., Hammong, C. N., & F.Schatz, P. (2010). Techniques in organic chemistry (Third Edit). United States of America

Mulyaningsih, A. P., Yetti, R. D., & Rivai, H. (2020). Phytochemical and pharmacological review of maja ( Aegle Marmelos ). World Journal of Farmacy and Pharmaceutical Science, 9(9), 19–42. https://doi.org/10.20959/wjpps20209-17045

Ng, R. C., Kassim, N. K., Yunie Soon, Y. Y., Gwendoline Cheng, L. E., Yazan, S. L., & Musa, K. H. (2018). Isolation of carbazole alkaloids and coumarins from aegle marmelos and murraya koenigii and their antioxidant properties. Sains Malaysiana, 47(8), 1749–1756. https://doi.org/10.17576/jsm-2018-4708-14

Nugroho, A. E., Riyanto, S., Sukari, M. A., & Maeyama, K. (2011). Anti-allergic effects of marmin, a coumarine isolated from Aegle marmelos Correa: In vitro study. International Journal of Phytomedicine, 3(1), 84–97

Riyanto, S. (2003). Pytochemical studies and bioactivity tests of murraya paniculata jack, Aegle marmelos Correa, and Zingiber amaricans Blume. University Putra Malaysia

Salempa, P. (2014). Isolasi dan identifikasi senyawa metabolit sekunder ekstrak n-Heksan daun tumbuhan maja ( Aegle marmelos Linn .) isolation and identification of secondary metabolites compound contained n-hexane extract plant leaves ( Aegle marmelos Linn .). Jurnal Sainsmat, III(2), 185–190

Sanghi, S. B., & Mushtaq, S. (2017). Aegle marmelos a potential medicinal tree: an overview. International Journal of Research Granthaalayah, 5(8), 63–66. https://doi.org/https://doi.org/10.29121/granthaalayah.v5.i8.2017.2183

Sari, M. P., & Susilowati, R. P. (1981). Larvacide effication test of maja (Aegle Mormelos) leaves powder on aedes aegypti. In L. L. Herlina (Ed.), Journal of Chemical Information and Modeling (Vol. 53, Issue 9, pp. 1689–1699). Research Centre Unit, Medical Faculty of Lambung Mangkurat University

Sastrohamidjojo, H. (1996). Sintetis bahan alam. Gadjah Mada University Press

Snehlata, K., Sheel, R., & Kumar, B. (2018). Evaluation of phytochemicals in polar and non polar solvent extracts of leaves of Aegle marmelos (L.). IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB), 4(5), 31–38. https://doi.org/10.9790/264X-0405013138

Subhasini, R. A. G. R. (2014). In-vitro anti-inflammatory and mosquito larvicidal efficacy of nickel nanoparticles phytofabricated from aqueous leaf extracts of Aegle marmelos Correa. Acta Tropica, 135(July 2014), 19–26. https://doi.org/10.1016/j.actatropica.2014.03.012

Svaefullah, B. L., Erwanto, Y., Wihandovo, Muhlisin, Prasetvo, R. A., Kurniawati, N., & Fitriyanto, N. A. (2020). Maja pruit extracts inhibit escherichia coli, reduce fly larvae population, and ammonia emission of chicken excreta. Tropical Animal Science Journal, 43(4), 369–376. https://doi.org/10.5398/tasj.2020.43.4.369

Tasyrifah, M. (2010). Efek pemberian ekstrak daun maja ( Aegle Marmelos Corr. ) terhadap fertilitas tikus betina. Institut Pertanian Bogor

Tobo, F., Mufidah, Taebe, B., & Mahmud, I. (2001). Buku pegangan laboratorium fitokimia I (ekstraksi komponen kimia bahan alam). Laboratorium Fitofarmasi Jurusan Farmasi, FMIPA Universitas Hasanuddin

Wilujeng, S., I, P. L., & Tiaranisa, P. (2020). Antioxidant maja fruit ( Aegle marmelos ( L ) Carrea ) lowering blood sugar mus musculus. Budapest International Research in Exact Sciences Medical, Biological, Argiculture, Enginering Science and Other Related Areas, Volume 2,(L), 362–367. https://doi.org/https://doi.org/10.33258/birex.v2i3.1075

Downloads

Published

2021-03-31

Issue

Vol. 11 No. 1 (2021): Pharmaciana

Section

Analytical Pharmacy and Medicinal Chemistry

License

Authors who publish with Pharmaciana agree to the following terms:

  1. Authors retain copyright and grant the journal the right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA 4.0) that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal. 
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.