The gastroprotective effects of arrowroot tuber starch (Maranta arundinacea L.) on ethanol-induced gastric damages in rats

Authors

  • Vella Lailli Muhammadiyah University of Yogyakarta
  • Moch Saiful Bachri Farmasi, Universitas Ahmad Dahlan
  • Wahyu Widyaningsih Farmasi, Universitas Ahmad Dahlan

DOI:

https://doi.org/10.12928/pharmaciana.v10i1.12318

Keywords:

arrowroot tuber (Maranta arundinacea L.), gastroprotective agent, histopathology

Abstract

Empirically, arrowroot tubers (Maranta arundinacea L.) have been widely used in the treatment of gastric ulcers. They are known to contain carbohydrates and flavonoids that play a role in reducing inflammation. This study sought to identify the gastroprotective effects of arrowroot tuber starch (Maranta arundinacea L.) on the ulcer index, % protection ratio, and the histopathological image of Wistar rat models of gastric ulcers. The test animals were divided into six groups. Group I was given free access to food and water (normal control), while Group II was given ethanol without treatment (negative control). Groups III, IV, and V were treated with arrowroot tuber starch at the doses of 125, 250, and 500 mg/kg BW, respectively. Group VI was given sucralfate at the dose of 400 mg/kg BW (positive control). All treatments were administered orally for 14 days and followed by 24 hours of fasting. On Day 15, all groups, except for the normal control, were given 96% ethanol orally at the dose of 1 ml/200gr BW. After one hour, they were dissected, and their stomach was removed for further analyses. The results showed that the administrations of arrowroot tuber starch at 125, 250, and 500 mg/kg BW produced ulcer indices of 2, 1.25, and 1.5, respectively, smaller than the negative control (4.25), and % protection ratios higher than the positive control. The histopathological imaging showed that the stomach of rats receiving arrowroot tuber starch at 250 mg/kg BW presented no pathological changes. Based on these findings, the arrowroot tuber starch is proven to have the ability as a gastroprotective agent.

References

Cipriani T.R., Caroline G.M., Lauro M. de Souza, Cristiane H.B., Cristina S.F., Maria C.A.M., Philip A.J.G., Guilherme L.S., Marcello I., 2009, Polygalacturonic acid: Another anti-ulcer polysaccharide from the medicinal plant Maytenus ilicifolia, Carbohydrate Polymers 78: 361–363.

Cordeiro L.M.C., Vanessa de Fátima Reinhardt, Cristiane H.B, Maria F.P.W., Ligia M.B., Guilherme L. Sassaki, M.I, 2012, Arabinan and arabinan-rich pectic polysaccharides from quinoa (Chenopodium quinoa) seeds: Structure and gastroprotective activity, Food Chemistry 130: 937–944.

Dashputre N.L, Naikwade N.S., 2011, Evaluation of Anti-Ulcer Activity of Methanolic Extract of Abutilon indicum Linn Leaves in Experimental Rats, International Journal of Pharmaceutical Sciences and Drug Research 3(2): 97-100.

Kalyana K.R., MD.FRCSE, Robert C.S., MD, 2007, Peptic Ulcer Disease, American Family Physician (76): 1005-12.

Kumar V, Z. A. Bhat, Dinesh K, N. A. Khan, I. A. Chashoo, Irfat A., 2012, Gastroprotective effect of leaf extracts of Basella alba var. Alba against experimental gastric ulcers in rats, Brazilian Journal of Pharmacognosy, 22(3): 657-662.

Kusbandari, Aprilia, 2015, Analisis Kualitatif Kandungan Sakarida Dalam Tepung Dan Pati Umbi Ganyong (Canna edulis Ker.), PharmaÒ«iana, 5(1): 35-42.

Priamsari M.R, Pujiastuti A., 2016, Pengaruh Biji Pati Avokad (Persea Americana, Mill) Terhadap Aktivitas Anti Ulcer Pada Lambung Tikus Jantan Galur Wistar, Indonesian Journal on Medical Science, 31(1): 22-28.

Nascimento A. M., Ferreira D. M., Souza E. J., Souza L.M., Sassaki G. L., Iacomini M., Cipriani T. R., 2017, Gastroprotective effect and chemical characterization of a polysaccharide fraction from leaves of Croton cajucara, International Journal of Biological Macromolecules, 95 :153-159.

Nordin Noraziah, Suzy M.S, Shahram Golbabapour, Maryam Hajrezaie, Pouya Hassandarvish, Behnam Kamalidehghan, Nazia A.M, Najihah M.H., Hanita Omar, Mehran Fadaienasab, Hamed Karimian, Hairin Taha, Hapipah M.A., Mahmood A.A., 2014, Anti-Ulcerogenic Effect of Methanolic Extracts from Enicosanthellum pulchrum (King) Heusden against Ethanol-Induced Acute Gastric Lesion in Animal Models, Plos One, 9(11): 1-13.

Okatiranti, 2011, Pengaruh Pemberian Ekstrak Daun Pegagan (Centella Asiatica) Pada Ketahanan Mukosa Lambung (Gastroprotektif) Tikus yang Mengalami Stres Immobilisasi, Majalah Obat Tradisional, 16(2), 81 – 87.

Pertiwi Reza, 2015, Efek Gastroprotektor Ekstrak Etanol Umbi Garut (Maranta Arundinacea L.) Terhadap Indeks Tukak Lambung, Rasio Proteksi, Ekspresi Protein COX 2 Dan Gambaran Histopatologi Lambung Pada Tikus Model Tukak Lambung, Master Thesis, Program Pasca Sarjana Universitas Ahmad Dahlan, Yogyakarta.

Rozzaa A.L., Thiago de M.M., Hélio Kushimab, Alexandre Tanimotoa, Márcia O.M.M., Taís M.B., Clélia A.H.L., Cláudia H.P, 2011, Gastroprotective Mechanisms of Citrus Lemon (Rutaceae) Essential Oil and its Majority Compounds Limonene And Β-Pinene: Involvement of Heat-Shock Protein-70, Vasoactive Intestinal Peptide, Glutathione, Sulfhydryl Compounds, Nitric Oxide and Prostaglandin E2, Chemico-Biological Interactions, 189: 82–89.

Saad B. Almasaudi,1 Nagla A. El-Shitany, Aymn T. Abbas, Umama A. Abdel-dayem, Soad S. Ali, Soad K. Al Jaouni and Steve Harakeh, 2016, Antioxidant, Anti-inflammatory, and Antiulcer Potential of Manuka Honey against Gastric Ulcer in Rats, Oxidative Med Cell Longevity, vol 2106: 1-10.

Saha Lekha, Alka Bhatia, Amitava Chakrabarti, 2016, Gastroprotective effect of bezafibrate, a peroxisome proliferator activated receptor a agonist and its mechanism in a rat model of aspirin-induced gastric ulcer, Advances in Digestive Medicine, 3: 101-110.

Shintu PV, Radhakrishnan VV, Mohanan KV, 2015, Pharmacognostic standardisation of Maranta arundinacea L - An important ethnomedicine, Journal of Pharmacognosy and Phytochemistry, 4(3): 242-246.

Syahrir N.H.A., Farit M.A., Budi Susetyo, 2016, Efek Sinergis Bahan Aktif Tanaman Obat Berbasiskan Jejaring Dengan Protein Target, Jamu Indonesia, 1(1):35-46.

Tarigan Pengarapen., 2009, Tukak Gaster, Dalam P. Tarigan, A. W. Sudoyo, B. Setiyohadi, I. Alwi, M. Simadibrata, and S. Setiati (Penyunt.), Buku Ajar Ilmu Penyakit Dalam, 5(1), Interna Publishing, Jakarta: 513-522.

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Published

2020-03-30

Issue

Vol. 10 No. 1 (2020): Pharmaciana

Section

Pharmacology

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