SKRINING FITOKIMIA EKSTRAK ETANOL Ulva lactuca L. DENGAN METODE KROMATOGRAFI LAPIS TIPIS
DOI:
https://doi.org/10.12928/mf.v13i2.7772Abstract
Ganggang hijau (Ulva lactuca L.) merupakan spesies ganggang laut yang berpotensi sebagai obat kardiovaskuler. Untuk mendukung pemanfaatannya perlu dilakukan skrining fitokimia kandungan ganggang hijau yang berasal dari pantai Drini, Daerah Istimewa Yogyakarta. Tujuan penelitian ini adalah melakukan skrining fitokimia Ulva lactuca yang berasal dari pantai Drini dengan Kromatografi Lapis Tipis (KLT). Ulva lactuca diekstraksi dengan metode maserasi dengan etanol 96%. Ekstrak yang diperoleh ditetapkan randemen,  kadar air, kadar abu dan kadar logam berat (Pb dan Cd). Penetapan kadar air dengan alat Hydrogen Moisturizer Analyzer (HMA), kadar abu dengan metode gravimetri dan penetapan logam berat Pb dan Cd dengan Spektrofotometer Serapan Atom (SSA). Skrining fitokimia dilakukan untuk mengindentifikasi golongan senyawa alkaloid, flavonoid, saponin, antrakuinon dan terpenoid dengan KLT. Identifikasi klorofil dilakukan dengan metode Nollet (2004). Hasil penelitian menunjukkan ekstrak etanol Ulva lactuca  mempunyai randemen 14,52%, kadar air 9,67±0,07%, kadar abu 34,05% , kadar logam Pb<0,07 mg/kg dan kadar logam Cd<0,01 mg/kg. Terdapat golongan senyawa flavonoid, alkaloid dan klorofil dalam  ekstrak etanol Ulva lactuca L. yang diperoleh dari pantai Drini.References
Abad Martinez, M.J., Del Olmo, L.M.B., dan Benito, P.B., 2005, Antiviral activities of polysaccharides from natural sources, dalam: Atta-ur-Rahman (Ed.), Studies in Natural Products Chemistry, Bioactive Natural Products (Part K). Elsevier, hal. 393–418.
Abirami, R.G. dan Kowsalya, S., 2011, Nutrient and Nutraceutical Potentials of Seaweed Biomass Ulva lactuca and Kappaphycus alvarezii, Journal of Agricultural Science and Technology, 5: 107–115.
BPPK Depkes RI, 2008, Laporan Nasional Riskesdas 2007, URL: https://www.k4health.org/sites/default/files/laporanNasional%20Riskesdas%202007.pdf (diakses tanggal 14/4/2016).
BPOM RI, 2014, Peraturan Kepala BPOM No. 12 Tahun 2014 tentang Persyaratan Mutu Obat Tradisional
Einav, R. dan Israel, A., 2007, Seaweeds on the Abrasion Platforms of the Intertidal Zone of Eastern Mediterranean Shores, dalam: Seckbach, J. (Ed.), Algae and Cyanobacteria in Extreme Environments. Springer Netherlands, Dordrecht, hal. 193–207.
El Gamal, A.A., 2010, Biological importance of marine algae. Saudi Pharmaceutical Journal : SPJ, 18: 1–25.
El-Baky, F.K.E.B., 2009, Potential biological properties of sulphated polysaccharides extracted from the macroalgae Ulva lactuca L. Cancer Research, 2: 1–11.
El-Baky, H.H.A., El-Baz, F.K., and El-Baroty, G.S., 2008, Evaluation of marine alga Ulva lactuca L as a source of natural preservative ingredient. American Eurasian Journal Of Agricultural And Environmental Science, 3: 434–444.
Elmegeed, D.F.A., Ghareeb, D.A., and El-Saadani, M., 2014, Phytochemical constituents and bioscreening activities of green algae (Ulva lactuca). International Journal of Agricultural Policy and Research, 2: 373–378.
Febriansah, E.M., Eka Sakti, E.R., dan Kodir, R.A., 2015. Uji Aktivitas Antioksidan Ekstrak Selada Laut (Ulva lactuca L) dengan Ekstraksi Bertingkat menggunakan Metoda DPPH. Prosiding Farmasi; Farmasi (Gel 2 Th Akad 2014-2015); 531-538.
Harborne, J., 1996. Metode Fitokimia : Penuntun Cara Modern Menganalisis Tumbuhan. Penerbit ITB, Bandung.
Kosanić, M., Ranković, B., dan Stanojković, T., 2015. Biological activities of two macroalgae from Adriatic coast of Montenegro. Saudi Journal of Biological Sciences, 22: 390–397.
Lerner, A., James, D., and Yoshiyata, T., 1960. Isolation of melatonin and 5-methoxyindole-3-acetic acid from bovine pineal glands. The Journal of Biological Chemistry, 7: 235.
Manchu, N., Melpha, Y., dan James, E., 2014. Phytochemical investigation of three species of Ulva from Rasthacaud Coast, Tamil Nadu, India. Journal of Chemical and Pharmaceutical Research, 6: 570–574.
Meenakshi, S., Gnanambigai, D.M., Mozhi, S.T., Arumugam, M., dan Balasubramanian, T., 2009. Total Flavanoid and in vitro Antioxidant Activity of Two Seaweeds from Rameshwaram Coast. Global Journal of Pharmacology, 3: 59–62.
Nollet, L., 2004. Handbook of Food Analysis Vol.1.Second Edition, Physical Characterization and Nutrien Analysis. Marcel Dekker Inc, New York.
Pádua, M. de, Fontoura, P.S.G., and Mathias, A.L., 2004. Chemical composition of Ulvaria oxysperma (Kützing) bliding, Ulva lactuca (Linnaeus) and Ulva fascita (Delile). Brazilian archives of biology and technology, 47: 49–55.
Paredes, S.D., Korkmaz, A., Manchester, L.C., Tan, D.-X., and Reiter, R.J., 2009. Phytomelatonin: a review. Journal of Experimental Botany, 60: 57–69.
Robic, A., Sassi, J.-F., and Lahaye, M., 2008. Impact of stabilization treatments of the green seaweed Ulva rotundata (Chlorophyta) on the extraction yield, the physico-chemical and rheological properties of ulvan. Carbohydrate Polymers, 74: 344–352.
Robinson, T., 1995. Kandungan Organik Tumbuhan Tinggi / Trevor Robinson ; Penerjemah Kosasih Padmawinata ; Penyunting Tetet Sutomo, VI. ed. ITB Press, Bandung.
Santos, A.., Guevera, B.., Mascardo, A.., dan Estrada, S.., 1978. Phytochemical, Microbiological and Pharmacological, Screening of Medical Plants. Research Center University of Santo Thomas, Manila.
Sathivel, A., Raghavendran, H.R.B., Srinivasan, P., and Devaki, T., 2008. Anti-peroxidative and anti-hyperlipidemic nature of Ulva lactuca crude polysaccharide on D-galactosamine induced hepatitis in rats. Food and Chemical Toxicology: An International Journal Published for the British Industrial Biological Research Association, 46: 3262–3267.
Sava, C. dan Sirbu, R., 2010. Analytical study of the determination of flavonoids in Black Sea algae. Ovidius University Annals of Chemistry, 21: 29–34.
Widyaningsih, W., Sativa, R., dan Primardiana, I., 2015a. Efek Antioksidan Ekstrak Etanol Ganggang Hijau (Ulva Lactuca L.) terhadap Kadar Malondialdehid (MDA) dan Aktivitas Enzim Superoksida Dismutase (SOD) Hepar Tikus yang Diinduksi CCL4. Media Farmasi, 12: 25–37.
Widyaningsih, W., Utami, P., Amalia, C.R., Amelia, S., Ramadhani, M.R., dan Retnosari, 2015b. Efek Apoptosis Ekstrak Etanol Ganggang Hijau (Ulva lactuca) pada Jantung Tikus yang Diinduksi Isoproterenol. Jurnal Farmasi dan Ilmu Kefarmasian, 2: 59–63.
Widyaningsih, W., Sugiyanto, Pramono, S., Widyarini, S., 2016, Pengkajian Aktivitas Kardiopotektif, Antioksidan dan Antiapoptosis Ekstrak Etanol Terstandar Melatonin dari Ganggang Hijau (Ulva Lactuca L), Disertasi, Universitas Gadjah Mada.
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