Efek Ekstrak Etanol Daun Kemangi (Ocimum sanctum L.) Sebagai Agen Kemopreventif Pada Sel Kanker Leher Rahim Hela Melalui Aktivitas Sitotoksik Dan Induksi Apoptosis

Authors

  • Nur Ismiyati Prodi D3 Farmasi Poltekkes Bhakti Setya Indonesia Yogyakarta
  • Farisya Nurhaeni Prodi D3 Farmasi Poltekkes Bhakti Setya Indonesia Yogyakarta

DOI:

https://doi.org/10.12928/mf.v13i1.5741

Abstract

Kanker leher rahim merupakan penyebab kematian wanita akibat kanker terbesar di negara-negara berkembang. Pertumbuhan sel kanker bersifat tak terbatas karena mempunyai kemampuan untuk menghindari mekanisme apoptosis sehingga penemuan agen-agen pemacu apoptosis kini terus dikembangkan. Kemangi (Ocimum sanctum L.) dilaporkan memiliki potensi penghambatan perkembangan sel kanker melalui aktivitas sitotoksik, apoptosis, dan penghambatan angiogenesis pada sel kanker paru dan payudara. Penelitian ini bertujuan untuk mengetahui efek ekstrak etanol daun kemangi terhadap aktivitas sitotoksik dan proses pemacuan  apoptosis pada sel kanker leher rahim HeLa. Evaluasi viabilitas sel melalui nilai IC50 ditetapkan dengan MTT assay. Efek pemacuan apoptosis oleh ekstrak etanol daun kemangi terhadap sel kanker HeLa diamati dengan metode pengecatan DNA menggunakan etidium bromida-akridin oranye. Hasil penelitian ini menunjukkan ekstrak etanol daun kemangi memiliki potensi sebagai agen kemopreventif pada kanker leher rahim HeLa dengan IC50 209µg/mL. Berdasarkan hasil tersebut dapat disimpulkan penghambatan pertumbuhan sel HeLa oleh ekstrak etanol daun kemangi terjadi dengan menginduksi terjadinya apoptosis.

 

Kata kunci : kanker leher rahim, sel HeLa, daun  kemangi, sitotoksik, apoptosis

 

References

Aziz, MF., Andrijono, Saifuddin AB, editors., 2006. Buku Acuan Nasional Onkologi Ginekologi. Edisi kedua. Jakarta: Yayasan Bina Pustaka Sarwono Prawirohardjo

Beck, W.T., Mo, Y.Y., Bhat, U.G., 2001, Cytotoxic Signaling by Inhibitor of DNA Topoisomerase II, Biochemical Society Transactions, 29 (6)

Davis, J.M., Navolonic, P.M., Weinstein, C.R., Steelman, L.S., Hu, Konovlepa, M., Blagosklonny M.V and McCubrey, J.A., 2003, Raf-1 and Bcl-2 Induce Distinct dan Commn Pathway That Contribute to Cancer Drug Resistance, Clinical Cancer Research, 9, 1161-1170.

DeFillippis, R.A., Goodwin, E.C., Wu, L., and DiMaio, D., 2003, Endogenous Human Papillomavirus E6 and E7 Proteins Differentially Regulate Proliferation, Senescence, and Apoptosis in Hela Cervical Carcinoma Cells, Journal of Virology,.77 (2) : 1551-1563.

Doyle, A., and Griffiths, J.B., 2000, Cell and Tissue Culture for Medical Research, John Willey and Sons Ltd, New York.

Kim, S.C., Magesh, V., Jeong, S.J., Ahn, K.S., Lee, H.J., Lee, E.O., Kim, S.H., Lee, M.H., Kim, J.H., 2010, Ethanol Extract of Ocimum sanctum Exerts Anti-metastatic Activity Through Inactivation of Matrix Metalloproteinase-9 and Enhancement of Anti-oxidant Enzymes, Food Chem Toxicol 48(6):1478-1482.

Magesh, V., Lee, J.C., Ahn, K.S., Lee, H.J., Lee, H.J., Lee, E.O., Shim, B.S., Jung, H.J., Kim, J.S., Kim, D.K., Choi, S.H., Ahn, K.S., Kim, S.H., 2009, Ocimum sanctum Induces Apoptosis in A549 Lung Cancer Cells and Suppresses the In Vivo Growth of Lewis Lung Carcinoma Cells, Phytother Res 23(10): 1385-1391.

McGahon, A.J., Martin, S.J., Bissonnette, R.P., Mahboubi, M., Shi, Y., Mogil, R. J., Nishioka, W. K., and Green, D. R., 1995, The End of the (Cell) Line: Methods for the Study of Apoptosis in Vitro, in: Schwartz, L. M., Osborn, B. A., Cell Death, Academic Press, San Diego.

Mosmann, T.R. 1983. Rapid colorimetric for cellular growth and survival: application to proliferation and cytotoxic assays. J. Immunol. Methods 65:55-58.

Nangia-Makker, P., et.al., 2007, Inhibition of breast tumor growth and angiogenesis by a medicinal herb: Ocimum sanctum, Int J Cancer. August 15; 121(4): 884–894. doi:10.1002/ijc.22733.

Nishizawa, M., Yasui, Y., Ito, M., Hirono, I., and Andoh, T., 2001, New Topoisomerase I Inhibitors, TAN-1518A and Ptaquiloside: Their Mode of Action, Laboratory of Biochemistry.

Pan, M.H., Chen, W.J., Lin-Shiau, S., Ho, C.H., and Lin, J.K., 2002, Tangeretin Induces Cell-Cycle Through Inhibiting Cyclin-Dependent Kinase 2 & 4 Activities As Well As Elevating Cdk Inhibitor p21 in Human Colorectal Carcinoma Cells, Carcinogenesis, Oxford University Press, 23: 1677-1684.

Rekam Medis RS Kanker Dharmais, 2012, Statistik Kanker : 10 Besar Kanker Tersering RSKD Rawat Jalan tahun 2007, diambil dari http://www. dharmais.co.id/index.php/cancer-statistic.htmL, diakses pada bulan Maret 2012.

Ribble, D., Goldstein, N.B., Norris, D.A., and Shellman, Y.G., 2005, A Simple Technique for Quantifying Apoptosis in 96-Well Plates, BMC Biotechnology, 5:12.

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Issue

Vol. 13 No. 1: Maret 2016

Section

Pharmaceutical & Technology

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