Aktivitas Penangkapan Radikal 2,2–Difenil–1–Pikrilhidrazil (DPPH) Oleh Ekstrak Metanol Paprika Merah (Capsicum annuum, L.)

Authors

  • Warsi Warsi Fakultas Farmasi Universitas Ahmad Dahlan
  • Any Guntarti Fakultas Farmasi Universitas Ahmad Dahlan

DOI:

https://doi.org/10.12928/mf.v13i1.5740

Abstract

Paprika merah (Capsicum annum L.) banyak mengandung β–karoten  (provitamin A), likopen, vitamin E serta vitamin C telah diuji aktivitasnya sebagai penangkap radikal 2,2–difenil–1–pikrilhidrazil (DPPH). Sampel diekstraksi dengan cara maserasi menggunakan metanol. Ekstrak yang diperoleh dilakukan analisis aktivitas antioksidannya secara kualitatif dengan larutan DPPH 0,4 mM dan reagen ferri tiosianat. Identifikasi terhadap senyawa yang berpotensi sebagai antioksidan dilakukan dengan spektrofotometer UV–Vis. Aktivitas penangkapan radikal DPPH oleh ekstrak metanol paprika merah ditetapkan secara spektrofotometri pada panjang gelombang 517 nm. Dari hasil analisis diperoleh nilai IC50 paprika merahsebesar0,299 ± 4,98x10-3 mg/mL. Aktivitas paprika merah sebagai penangkap radikal DPPH  kurang poten dibandingkan dengan β–karoten (IC50 0,066 ± 3,24x10-3 mg/mL).

 

Kata kunci : antioksidan, paprika merah, Capsicum annuum, DPPH

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Issue

Vol. 13 No. 1: Maret 2016

Section

Pharmaceutical & Technology

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