Pengaruh Pemakaian PEG 400 Dan Mentol Dalam Patch Mukoadhesif Ekstrak Etanol Daun Sirih (Piper betle L.) Terhadap Transpor Senyawa Polifenol

Authors

  • Setyawan E I Jurusan Farmasi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Udayana
  • P O Samirana Jurusan Farmasi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Udayana
  • I G A. Indyayani Jurusan Farmasi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Udayana

DOI:

https://doi.org/10.12928/mf.v13i1.5738

Abstract

Patch bukal merupakan sediaan obat dengan sistem penghantaran transmukosa. Kemampuan absorpsi zat aktif sediaan Patch bukal dapat dimodifikasi dengan penambahan suatu plasticizers dan permeation enhancer.Penelitian ini dilakukan untuk mengetahui pengaruh pemakaian PEG 400 dan mentol terhadap transpor serta mengetahui proporsi PEG 400 dan mentol yang dapat memberikan transpor optimum senyawa polifenol ekstrak daun sirih (Piper bitle L.) dari patch bukal secara in vitro. Metode yang digunakan untuk menentukan perbandingan jumlah PEG 400 dan mentol adalah dengan metode Simplex Lattice Design (SLD). Uji transpor senyawa polifenol dari patch dilakukan selama 23 jam dengan menggunakan sel difusi Franz dengan membran Whatmann No. 1. Jumlah senyawa yang tertranspor ditetapkan kadarnya dengan instrumen Spektrofotometer-UV. Verifikasi formula optimum dilakukan sebanyak 3 kali dengan Single Simple Test. Hasil penelitian menunjukkan bahwa penggunaan PEG 400 dan mentol dapat meningkatkan jumlah polifenol yang tertranspor. Hubungan pengaruh PEG 400 dan mentol dapat digambarkan melalui persamaan special cubic, y =  3,26071 (A) + 4,31372 (B) + 2,80876 (A)(B) + 0,64912 (A)(B) (A-B). Verifikasi formula optimum menghasilkan p-value 0,503(>0,05). Permodelan transpor polifenol melalui membran dapat digambarkan dengan model 4 kompartemen dan menghasilkan kecepatan masing-masing L(2,1) sebesar 0,013 mgEAG/cm2/jam, L(3,2) sebesar 0,050 mgEAG/cm2/jam, L(4,1) sebesar 0,089 mgEAG/cm2/jam, dan L(3,4) sebesar 0,027 mgEAG/cm2/jam.

 

Kata kunci: Piper betle, mentol, Patch bukal, PEG 400, polifenol.

References

Anna, P., and Krystyana, P., 2014, Evaluation of Aluminium Complexation Reaction for Falavonoid Content Assay, Food Anal. Methods, (7) : 1776-1782.

Hernani dan R. Nurdjanah, 2009, Aspek Pengeringan dalam Mempertahankan Kandungan Metabolit Sekunder pada Tanaman Obat, Perkembangan Teknologi TRO, 21 (2) : 33-39.

International Conference on Harmonisation, 2012, Guidance for Industry, International Conference on Harmonisation, USA : 3-7.

Jia-You FANG, Tung-Hu TSAI, Yu-Ying LIN, Wen-Wen WONG, Meng-Nan WANG, and Jeng-Fen HUANG, 2007, Transdermal Delivery of Tea Catechins and Theophylline Enhanced by Terpenes: a Mechanistic Study, Bio.Pharm. Bull., 30(2), 343-349.

Jinghua,Y., S. Peter, and H. Stephen, 2001, Effect of Polyethylen Glycol on Morphology Thermomechanical Properties and Water Vapor Permeability of Cellulose Acetate Free Film, Pharm.Tech., 423 : 62-73.

Kridati, E. M., E. Prihastanti, dan S. Haryanti, 2012, Rendemen Minyak Atsiri dan Diameter Organ serta Ukuran Sel Minyak Tanaman Adas (Foeniculum vulgare Mill) yang Dibudidayakan di Kabupaten Semarang dan Kota Salatiga, Buletin Anatomi dan Fisiologi, 20 (1) : 1-17.

Margetts, Lyn and R. Sawyer, 2007, Transdermal Drug Delivery: Principles and Opioid Therapy, The Board of Management and Trustees of the British Journal of Anaesthesia, 7 (5) : 171-175.

Palaska, I., E. Papathanasiou, T. C. Theoharides, 2013, Use of polyphenol in Periodontal Inflammation, Eur.J.Pharmacol., 720 : 77-83.

Pathan, I.B. and C.M. Setty, 2009, Chemical Penetration Enhancers for Transdermal Drug Delivery Systems. Trop.J.Pharm.Res., 8(2) : 173-179.

Putri, T.A. dan Farida, Y., 2013, Total Phenolic, Flavonoids Content and Antioxidant Activity of The Ethanolic Extract of Betel Leaf (Piper betle L.), The International Conference in Nanotechnology in Jakarta.

Rostiana, O., S.M. Rosita, dan D. Sitepu, 1992, Keanekaragaman Genotipa Sirih (Piper betle L.) Asal dan Penyebaran, Warta Tumbuhan Obat Indonesia, 1 (1).

Setyawan, E.I., Dewantara, I.G.N.A., Putra, I.M.D.D., 2014, Optimasi Formula Matrik Patch Mukoadhesif Ekstrak Daun Sirih (Piper betle L.) Menggunakan Mentol Dan PEG 400 Sebagai Permeation Enhancer Dan Plasticizer, Media Farmasi UAD, 11 (2) : 120-132.

Stefanovski D. Peter J. M. dan Raymond C. B, 2003, WinSAAM: A Windows-Based Compartmental Modeling System, Metabolism, 52 (9) : 1153-1166.

Subashkumar, R., Sureshkumar, M., Babu, S., and Thayumanavan, T., 2013, Antibacterial Effect of Crude Aqueous Extract of Piper betle L. Against Pathogenic Bacteria, Int. J. Res. Pharm. Biomed. Sci., 4 (1) : 42-46.

Wu, X., Desai, K.G.H., Mallery, S.R., Hulpoch, A.S., Pelps, M.P., and Schwendeman, S.P., 2012, Mucoadhesive Fenretinide Patches for Site-specific Chemoprevention of Oral Cancer: Enhancement of Oral Mucosal Permeation of Fenretinide by Co-incorporation of Propylene Glycol and Menthol, Mol. Pharm., 9 (4) : 937-945.

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Issue

Vol. 13 No. 1: Maret 2016

Section

Pharmaceutical & Technology

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