Antioxidant activities of extracts, methanol, and n-hexane partitions of cayenne pepper (Capsicum frutescens L.)

Authors

  • Slamet Slamet Departemen Herbal Medicine Program Sarjana Farmasi FIKES, Universitas Muhammadiyah Pekajangan Pekalongan http://orcid.org/0000-0003-4103-2327
  • Urmatul Waznah Departemen Herbal Medicine Program Sarjana Farmasi FIKES Universitas Muhammadiyah Pekajangan Pekalongan
  • Yusrilia Yusrilia Departemen Herbal Medicine Program Sarjana Farmasi FIKES, Universitas Muhammadiyah Pekajangan Pekalongan

DOI:

https://doi.org/10.12928/mf.v19i1.19093

Keywords:

Antioxidant, Cayenne pepper (Capsicum frutescens L.), partition, FRA

Abstract

Antioxidants are substances that can neutralize and/or stabilize free radicals. Cayenne pepper (Capsicum frutescens L.) is a natural source of antioxidants because it contains carotenoids, flavonoids, and vitamin C. This study aims to examine antioxidant activities of n-hexane, methanol, and extract of cayenne pepper (Capsicum frutescens L.) using the ferric reducing antioxidant power (FRAP) method. The extraction method was done by maceration, and the fractionation method was done by partitioning using unmixed solvents: methanol and n-hexane. The phytochemical screening test on extracts and partitions has discovered positive content of alkaloids, flavonoids, phenols, and terpenoids. Meanwhile, the TLC test used F254 silica gel as a stationary phase with chloroform: ethyl acetate as a mobile phase (6:4). The test discovered positive results containing flavonoids and phenols. Furthermore, the antioxidant activities were tested using the FRAP method with a UV-Vis spectrophotometer instrument at a maximum value of 598.05 nm and positive control of vitamin C. The results of measurement show that antioxidant activities are found in 505.67 ± 0.56 mol Fe2+/g of partition extract, 447.67 ± 16.2 mol Fe2+/g of methanol fraction, and 353± 5.29 mol Fe2+/g of n-hexane fraction.

Author Biography

Slamet Slamet, Departemen Herbal Medicine Program Sarjana Farmasi FIKES, Universitas Muhammadiyah Pekajangan Pekalongan

Departemen Herbal Medicine Program Sarjana Farmasi FIKES UMPP

References

Abozed, S. S., El-kalyoubi, M., Abdelrashid, A., & Salama, M. F. (2014). Total phenolic contents and antioxidant activities of various solvent extracts from whole wheat and bran. Annals of Agricultural Sciences, 59(1), 63-67. https://doi.org/10.1016/j.aoas.2014.06.009

Adhayanti, I., T. A. dan, & Romantika, R. (2012). Uji kandungan total polifenol dan flavonoid ekstrak etil asetat kulit pisang raja ( Musa paradisiaca var. sapientum ). Media Farmasi, 1, 146-152.

Firdaus, Muhammad., Prihatno, A. A. (n.d.). Tanaman bakau biologi dan bioaktivitas. Universitas Brawijaya Press.

Gurnani, N., Gupta, M., Shrivastava, R., Mehta, D., & Mehta, B. K. (2016). Effect of extraction methods on yield, phytochemical constituents, antibacterial and antifungal activity of Capsicum frutescens L. Indian Journal of Natural Products and Resources, 7(1), 32-39.

Hanani, E. (2015). Analisis Fitokimia. EGC.

Kusnadi, J., Wuri Andayani, D., & Zubaidah, E. (2019). Ekstraksi senyawa Bioaktif cabai Rawit (Capsicum Frutescens L.) menggunakan metode ekstraksi gelombang Ultrasonik. Jurnal Teknologi Pertanian, 20(2), 79-84. https://doi.org/10.21776/ub.jtp.2019.020.02.1

Marjoni, M.R, Afrinaldi, N. A. D. (2015). Kandungan total fenol dan aktivitas antioksidan ekstrak air daun Kersen (Mutingia calabura L). Jurnal Kedokteran Yarsi, 23, 187-196.

Pratama, M., Muflihunna, A., & Octaviani, N. (2018). Analisis aktivitas antioksidan sediaan propolis yang beredar di kota makassar dengan metode frap (Ferric Reducing Antioxidant Power). Jurnal Ilmiah As-Syifaa, 10(1), 11-18. https://doi.org/10.33096/jifa.v10i1.312

S.M., A. (2017). Kiat Sukses Budidaya Cabe Rawit (T Arfin Mahardika (ed.); 1st ed.).

Selawa, W., Revolta, M., Runtuwene, J., Citraningtyas, G., Studi, P., Fmipa, F., & Manado, U. (2013). Kandungan flavonoid dan kapasitas antioksidan total ekstrak etanol daun Binahong [Anredera cordifolia(Ten.)Steenis.]. Pharmacon, 2(1), 18-23.

Syarif, S., Kosman, R., Inayah, N. (2015). Uji aktivitas antioksidan Terong Belanda (Solanum betacum Cav.) dengan metode FRAP. As-Syifaa, 7, 26-33.

Wirasti. (2019). Penetapan kadar fenolik total, flavonoid total, dan uji aktivitas antioksidan ekstrak daun Benalu Petai (Scurrula atropurpurea Dans.) beserta penapisan Fitokimia. Journal of Pharmaceutical and Medicinal Sciences, 4(1), 1-5.

Yuslianto, E. R. (2018). Pengantar radikal bebas dan antioksidan. Deepublis.

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Published

2022-03-24

Issue

Vol. 19 No. 1: March 2022

Section

Pharmacology & Biology

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