Validasi Metode Analisis β-Karoten Dalam Ekstrak Etanol 96% Spirulina maxima Dengan Spektrofotometri Visibel

Authors

  • Siti Fatmawati Fatimah Fakultas Farmasi Universitas Ahmad Dahlan Yogyakarta
  • Vani Aisyah Fakultas Farmasi Universitas Ahmad Dahlan Yogyakarta
  • Laela Hayu Nurani Fakultas Farmasi Universitas Ahmad Dahlan Yogyakarta
  • Citra Ariani Edityaningrum Fakultas Farmasi Universitas Ahmad Dahlan Yogyakarta

DOI:

https://doi.org/10.12928/mf.v15i1.12354

Keywords:

Spirulina maxima, β-karoten, validasi metode analisis, spektrofotometri visibel

Abstract

Validasi metode analisis (VMA) penentuan kandungan β-karoten dalam Spirulina maxima perlu dilakukan agar didapatkan hasil yang valid dan dapat dipertanggung jawabkan secara ilmiah. Tujuan penelitian adalah membuktikan metode analisis penetapan kadar β-karoten dalam ekstrak etanol 96% Spirulina maxima menggunakan spektrofotometer visibel memenuhi syarat validitas. Parameter yang digunakan dalam VMA diantaranya spesifisitas, linieritas, akurasi, presisi, limit deteksi dan limit kuantitas. Syarat yang harus dipenuhi ditiap parameter yaitu nilai korelasi (r) ≥ 0,98 untuk linieritas, persen perolehan kembali masuk dalam range 98-102% dan nilai RSD ≤ 2% untuk akurasi, nilai RSD ≤ 2% untuk presisi, profil spektragram spektrofotometri visibel yang sama antara sampel dan standar untuk uji spesifisitas. Hasil penelitian dengan parameter validasi diantaranya linieritas dengan nilai r=0,998, persen perolehan kembali dalam rentang 98-101%, presisi keberulangan sistem dengan RSD 1,14 %, presisi antara 0,99 % dan uji spesifisitas sesuai profil spektragram spektrofotometri visibel yang sama antara sampel dan standar. Nilai LOD yang diperoleh yaitu 1,656 ug/mL dan LOQ 5,017 ug/mL. Berdasarkan hasil tersebut maka analisis penetapan kadar β-karoten dalam ekstrak etanol 96% Spirulina maxima dengan metode spektrofotometri visibel memenuhi syarat validitas menurut Petunjuk Operasional Penerapan CPOB tahun 2013.

Author Biography

Siti Fatmawati Fatimah, Fakultas Farmasi Universitas Ahmad Dahlan Yogyakarta

Departemen Teknologi Farmasi UAD

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Published

2018-03-01

Issue

Vol. 15 No. 1: Maret 2018

Section

Pharmaceutical & Technology

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