Effect of the ethanolic extract of red roselle calyx (Hibiscus sabdariffa L.) on hematocrit, platelets, and erythrocytes in healthy volunteers

Siti Setianingsih, Laela Hayu Nurani, Abdul Rohman

Abstract


Roselle calyx (Hibiscus sabdariffa L) is widely used as an antioxidant, antihepatotoxic, diuretic, anti-cholesterol, and immunostimulant. Roselle contains anthocyanin and quercetin that have antioxidant activity. This study aimed to determine the effects of the ethanolic extract of roselle calyx on hematocrit, platelets, and erythrocytes in healthy volunteers during the 30-day administration and 15 days after it ceased. This study employed pre- and post-treatment design on 21 healthy volunteers, comprising 11 males and 10 females who fulfilled the inclusion criteria and filled out the informed consent form willingly. Volunteers were provided with roselle capsules for 30 days with a dosage of 500 mg a day taken 10-15 minutes after dinner. The hematologic examination was performed on Day 0, 31, and 45, including the measurement of hematocrit, platelet, and erythrocyte levels using a hematology analyzer. The obtained data were analyzed in SPSS with paired t-test and Wilcoxon test. The results showed that the hematocrit, platelets, and erythrocytes of all male and female volunteers on Day 0, 31, and 45 were within the normal range. The comparison analysis affirmed that there was no significant difference between these three parameters from Day 0 to 31, Day 31 to 45, and Day 0 to 45 (p>0.05). As a conclusion, the administration of the ethanolic extract of roselle calyx with a dosage of one 500mg capsule per day for 30 days did not affect the hematocrit, platelets, and erythrocytes in healthy volunteers. There was also no delayed effect on these three hematological parameters 15 days after the administration stopped.


Keywords


Hibiscus sabdariffa; hematology; healthy volunteers; capsule;safety

Full Text:

PDF

References


Amercian Heart Association (AHA), 2015, Target Heart Rates. http://www.heart.org/HEARTORG/HealthyLiving/PhysicalActivity/FitnessBasics/Target-Heart-Rates_UCM_434341_Article.jsp, Diakses tanggal 15 Agustus 2017

Amiruddin,M.A., 2015, Analisa Hasil Pengukuran Tekanan Darah Antara Posisi Duduk dan Posisi Berdiri pada Mahasiswa Semester VII (Tujuh), Tugas Akhir, Fakultas Kedokteran, Universitas Sam Ratulangi, http://ejournal.unsrat.ac.id/index.php/ebiomedik/article/view/6635 diakses tanggal 5 Oktober 2017

Berman, A, Shirlee, S, & Barbara, 2009, Buku Ajar Praktik Keperawatan Klinik, Ed. ke-5. EGC, Jakarta

Badan Pengawas Obat Dan Makanan Republik Indonesia (BPOM RI), 2014, Peraturan Kepala Badan Pengawas Obat dan Makanan, Pedoman Pelaksanaan Uji Klinik Obat Herbal, Nomor 13, Jakarta.

Badan Pengawas Obat dan Makanan Republik Indonesia (BPOM RI), 2015. Peraturan Kepala Badan Pengawas Obat Dan Makanan No. 16 Tahun 2015 Tentang Tata Laksana Dan Penilaian Obat Pengembangan Baru. Jakarta

Chu, S.C. dan Chen, C, 2006, Effects of origins and fermentation time on the antioxidant activities of Kombucha, Food Chemistry 98: 502-507.

Da-Costa-Rocha I, Bonnlaender B, Sievers H, Pischel I, Heinrich M, 2014, Hibiscus sabdariffa L.-A phytochemical and pharmacological review, Food Chemistry, 165 hh 424-443.

Dahlan, S., 2014, Statistik untuk Kedokteran dan Kesehatan, Ed.6, Epidemiologi Indonesia, Jakarta.

Debon, R.B., Esther R.G., Salvador F.A., Oriol, S.C., Francesco A.M., Massucci, 2015, The Acut Impact Of Polyphenols from in Metabolic Homeostasis : an approach combining metabolomics and gene-expression analyses, Royal Society of Chemistry.

Ganong, W. F., 1995, Buku Ajar Fisiologi Kedokteran, Jakarta: EGC Penerbit Buku Kedokteran.

Gunawan, Bambang. 2007. Stres dan Sistem Imun Tubuh: Suatu Pendekatan Psikoneuroimunologi. Jurnal Sub Bagian Alergi Imunologi. Vol. 13.

Harioso, H, A, Kodrat I, S, & Adian F, R 2009, Perancangan Aplikasi Pengirim Pesan (SMS Broadcast) Berbasis Web, Skripsi, Fakultas Teknik Universitas Diponegoro, Semarang.

Hayati, Farida, 2013, Uji Praklinik dan Uji Klinik Fase I Ekstrak Air Akar Pasak Bumi (Eurycoma longifolia, Jack) Terstandar sebagai Afrodisiaka, Disertasi, Fakultas Kedokteran Universitas Gadjah Mada, Yogyakarta.

Hidayati Mukhani Dwi., Sussi Astuti., Maria Erna Kustyawati, 2014, Pengaruh Pemberian “Kombucha†Teh Rosella Terhadap Profil Darah Mencit (Mus musculus L.), Fakultas Pertanian, Universitas Lampung, AGRITECH, 34 No. 4

ICH, 2010, M3(R2) Nonclinical Safety Studies for the Conduct of Human Clinical Trials and Marketing Authorization for Pharmaceuticals. Guidance for Industry. U.S. Department of Health and Human Services Food and Drug Administration.

https://www.fda.gov/downloads/drugs/guidances/ucm073246.pdf

JNC VII, Development Of health and Human Services, 2004, Join National Committee On Pevention, Detection, Evaluation and Marketing Authorization for Pharmaceuticals, Guidance for Industry, U.S Departement of Health and Human Services Food and Drug Administration.

Kementrian Kesehatan Republik Indonesia (Kemenkes), 2011, Pedoman Interpretasi Data Klinik, Kementrian Kesehatan Republik Indonesia, Jakarta.

Kumalasari, E., 2017, Evaluasi Sediaan Kapsul Ekstrak Etanol Akar Pasak Bumi (Eurycoma longifolia J) dan Pengaruhnya terhadap Tanda Vital Sukarelawan Sehat, Tesis, Program Pascasarjana, Universitas Ahmad Dahlan, Yogyakarta.

Kusumastuti, Intan Ratna, 2014, Roselle (Hibiscus sabdariffa linn) Effects on Lowering Blood pressure as a Treatment for Hypertension, Artikel Review, Faculty of Medicine, Universitas Lampung, 3 Nomor 7.

Laurence, D.R., & A. L, Bacharach, 1964, Evaluatio of drug activities: pharmacometrics, 1th ed. Academic Press. London.

Mardiah., Lia Amalia., dan Agus Sulaeman, 2010, Ekstraksi Kelopak Bunga Rosella dan Batang Bunga Rosella (Hibiscus sabdariffa L.) sebagai Pewarna Merah Alami, Jurnal Pertanian ISSN 2087-4936 Volume 1 Nomor 1, Fakultas Agribisnis dan Teknologi Pangan, Jurusan Teknologi Pangan dan Gizi, Universitas Djuanda, Bogor.

Mardiyah., Sawarni H., MT, Reki Wicaksono Ashadi, dan Arifah Rahayu., 2009 Budidaya dan Pengolahan Rosela Merah Segudang Manfaat, Agro Media Pustaka : Jakarta.

Morton, J.F., 1999, Roselle Hibiscus sabdarifa L. http:// www.transgenica.com/database/h/hibiscus.htm. [19 Juni 2017].

Munawaroh, Siti., 2013, Pengaruh Ekstrak Kelopak Rosella (Hibiscus sabdariffa) terhadap Peningkatan Jumlah Eritrosit dan Kadar Hemoglobin (Hb) dalam darah Tikus Putih (Rattus nurvegicus) Anemia, Skripsi, Fakultas Sains dan Teknologi, Universitas Islam Negeri, Malang.

National Kidney Foundation, 2012, KDOQI Clinical Practice Guideline for Diabetes and CKD: 2012 UPDATE. American Journal of Kidney Disease, https://www.kidney.org/sites/default/files/docs/diabetes-ckd-update-2012.pdf

Nurkhasanah, 2015, The Effect Of Rosella (Hibiscus sabdariffa L) Treatment on IL-10 And IL-14 Secretion On Dimethylbenz (A) Anthracene (DMBA) Induced Rat International Journal Of Pharmacy And Pharmaceutical Sciences, Vol 7, Issue 4

Santoso, B., Suryawati,S., Saleh Danu,S, 2006, Evaluasi Khasiat dan Keamanan Obat (Uji klinik), Dalam Farmakologi Klinik dan Farmakoterapi, 183-9. Universitas Gadjah Mada, Yogyakarta.

Sari, Fita., Nurkhasanah., Moch Saiful Bahri, 2016, Uji Toksisitas Akut Ekstrak Etanol Kelopak Rosella (hibiscus sabdariffa l.) pada Tikus Sprague Dawley, Traditional Medicine Journal, Fakultas Farmasi, Universitas Ahmad Dahlan, Yogyakarta.

Sembiring T, Agustian L, dan Ariani, 2009, Peran Zinkum Terhadap Pertumbuhan Anak, Departemen Ilmu Kesehatan Anak, Fakultas Kedokteran, Universitas Sumatera Utara, Sari Pediatri Volume XI, Nomor 4;244-249.

Shepherd S.M, 2007 Dengue fever [serial online] (diunduh 23 april 2017), http://.www.emedicine.medscape.com.

Suwandi, T, 2012. Pengembangan Potensi Antibakteri Kelopak Bunga Hibuscus Sabdariffa L Terhadap Streptococcis Sanguinis Penginduksi Ginvitis Menuju Obat Herbal Terstandar. Disertasi, Fakultas Kedokteran Gigi (FKG UI), Jakarta

Swenson, M.J, 1997. Duke’s Physiology of Domestic Animal. Comstock. Publ. Co. Inc, Ithaca New York.

Tisnadjaja D, 2006, Bebas Kolesterol dan Demam Berdarah dengan Angkak, Jakarta: Penebar Swadaya.

Virden, W.S., M.S. Lilburn, J.P., Thaxton, A., Corzo, D., Hoehler and M.T. Kidd., 2007, The Effect of Corticosterone-Induced Stress on Amino Acid Digestibility in Ross Broilers, Poult. Sci, 86 : 338 – 342.

Wardhana, April H, E Kenanawati, Nurmawati, Rahmaweni, dan C.B. Jatmiko. 2001. Pengaruh Pemberian Sediaan Patikaan Kebo (Euphorbia hirta L) terhadap Jumlah Eritrosit, Kadar Hemoglobin, dan Nilai Hematokrit pada Ayam yang Diinfeksi dengan Eimeria tenella. Jurnal Ilmu Ternak dan Veteriner. Vol. 6 No. 2 Th. 2001. Bogor.

Wulandari, Brilianti Dwi., 2010, Pengaruh Pemberian Seduhan Kelopak Bunga Rosella (Hibiscus sabdariffa) Dosis Bertingkat Selama 30 Hari terhadap Gambaran Histologik Ginjal Tikus Wistar, Artikel Ilmiah, Fakultas Kedokteran, Universitas Diponegoro, Semarang.

Wulangi KS, 1993, “Prinsip-Prinsip Fisiologi Hewanâ€, Proyek Pembinaan Tenaga Kependidikan. Jakarta: Departemen pendidikan dan Kebudayaan Direktur Jenderal Perguruan Tinggi.

Xu, Y., Zhao, H., Zhang, M., Li, C.J., Lin, X.Z., Sheng J. dan Shi W., 2011, Variations of antioxidant properties and NO scavenging abilities during fermentation of tea, International Journal of Molecular Sciences 12: 4574- 4590.

Yulinah, E., Wahyuningsih, S. & R, K., 2015, Efek Diuretik Ekstrak Air Kelopak Bunga Rosella (Hibiscus sabdariffa L.) pada Tikus Wistar Jantan, J Pharm Sci Pharm Pract.

Zunilda SB., Arini Setiawati F.D. Suyana, 2003, Pengantar Farmakologi, Dalam Farmakologi dan Terapi, FKUI: 1-23.




DOI: http://dx.doi.org/10.12928/pharmaciana.v8i2.8738

Refbacks

  • There are currently no refbacks.


Copyright (c) 2021 Siti Setianingsih, Laela Hayu Nurani, Abdul Rohman

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.


 
Pharmaciana
ISSN Print: 2088-4559 | ISSN Online: 2477-0256
Website: http://journal.uad.ac.id/index.php/PHARMACIANA
Office: Faculty of  Pharmacy, Universitas Ahmad Dahlan
Jl. Prof. Dr. Soepomo, S.H., Janturan, Warungboto, Umbulharjo, Yogyakarta, Indonesia
Kode pos 55164
Email: pharmaciana@pharm.uad.ac.id