EFEK PROTEKTIF EKSTRAK ETANOL HERBA PEGAGAN (Centella asiatica (L.) Urban) TERHADAP PEMBENTUKAN KATARAK TIKUS WISTAR YANG DIINDUKSI SODIUM SELENIT

Abstract

Cataract is one of the eye pathology that can cause blindness. Oxidative stress
has been identified as an initiating factor of cataract. The objective research was to
study the protective effect of ethanol extract Centela asiatica toward cataract formation
on Wistar rat induced by sodium selenite. Cataract was induced in 9 days old Wistar
rat, which 5 groups of 7 each. Group I got injection aquadest and the test groups got
injection CMC-na (group II), vitamin E dose 378 IU/kg bw (group III), ethanol extract
Gotu cola herb dose 100 mg/kg bw (group IV), and 200 mg/kg bw (group V), 400
mg/kgbw, subcutaneously, respectively, for 3 days. After 4 hours, all rats got injection a
single dose 50 ug/kg of BW sodium selenite intraperitoneally. Then eyes were observed
in all groups on postnatal day 16, when the eyes of the rat first opened. Then percentage
of cataract incidence were calculated. Finally, rats were executed and eyes were taken
for lens histophatological analyses with hematoxiline eosin staining. Based on
macroscopic and microscopic analyses it can be concluded that ethano extract of
Centella asiatica had protective effect toward cataract formation on Wistar rat induced
by sodium selenite. The least cataract incidence was showed at dose 200 mg/kg bw.