Pembuatan Kolom Monolit Mixed-Mode untuk Pemisahan Fenol dalam Kromatografi Cair Sistem Kapiler

Authors

  • Aster Rahayu Universitas Ahmad Dahlan
  • Joni Aldilla Fajri Universitas Islam Indonesia
  • Lee Wah Lim Gifu University
  • Toyohide Takeuchi Gifu University

DOI:

https://doi.org/10.26555/chemica.v5i2.12282

Keywords:

Monolit polimer, Pentaeritrytol Tetraacrylate-Ethylene dimathacrylate, Mixed-mode Liquid Chromatography, HILIC, Reverse Phase Liquid Chromatography

Abstract

A Mixed-Mode monolithic column prepared from Pentaeritrytol Tetraacrylate (PETRA) and Ethylene dimathacrylate (EDMA) was investigated and used in capillary liquid chromatography. The polymer composition was optimized by varying percentage ratio of monomer and porogens. Column A with rasio 35/65 (monomer/porogen) showed the optimum ratio. The polymerization of acrylic groups were conducted by in-situ preparation in capillary fused silica. Three phenol compounds were separated using this monolithic column using acetonitrile 100% as eluent. Monolith PETRA-EDMA also could work to separate polar and non-polar compound simultaneously by HILIC mode using acetonitrile 90% as eluent. The morphology surface of monolith was observed by scanning electron microscope (SEM) and mechanical stability in term of pressure drop was observed as well.

Author Biographies

Aster Rahayu, Universitas Ahmad Dahlan

Chemical Engineering Department

Joni Aldilla Fajri, Universitas Islam Indonesia

Environmental Engineering Department

Lee Wah Lim, Gifu University

Department of Chemistry and Biomolecular Science

Toyohide Takeuchi, Gifu University

Department of Chemistry and Biomolecular Science

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Published

2018-12-31

Issue

Vol. 5 No. 2 (2018): Desember 2018

Section

Articles

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