Effect of Spirulina platensis suspension on quality and quantity of sperm and expression of caspase-3 of testis in diabetic wistar rats induced by streptozotocin

Ayu Shabrina, Kintoko Kintoko, Sapto Yuliani, Adhi Gunawan


Diabetes is metabolic disorders caused by oxidative stress that has always been linked to male reproductive dysfunction. Spirulina platensis has been used for therapeutic supplements for diabetes and repairing reproduction organs based on its antioxidant effects. The aim of this study was to examine the effect of Spirulina platensis suspension (SP) on quality and quantity of sperm also the expression of caspase-3 on testis in male Wistar rats induced by streptozotocin (STZ). This study used 30 adult male Wistar rats that divided into 6 groups of K1 (normal), K2 (single injection of STZ 45 mg/kg BW), K3 (normal rats treated with SP 36/kg BW/day) and treatment group K4, K5 and K6 were diabetic rats given with SP 36 mg/kgBW, 72 mg/kgBW and 144 mg/kg BW. The treatment was given orally for 28 days. Blood glucose level levels were measured using the biochemical analyzer. Quality and quantity of sperm were analyzed using Neubauer slide under the microscope. Apoptosis of Leydig cells was detected by immunohistochemical staining using Caspase-3 polyclonal antibodies. The result showed that there were significant differences between groups (p < 0.05) in final blood glucose level, quality, and quantity of spermatozoa and expression of caspase-3. Our findings clearly highlight the role of Spirulina platensis as an antidiabetes and protective agent for reproducton organs of STZ-induced in diabetic male Wistar rats that improves quality and quantity of sperm cells and reproductive organs.


Male infertility; diabetes mellitus; stress oxidative; Spirulina platensis

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Al-Dhabi, N. A., Arasu, M.V., 2016. Quantification of phytochemicals from commercial Spirulina products and their antioxidant activities, Hindawi, 2016: 1-13.

Amaral, S., Moreno, A. J., Santos, M. S., Seiça, R., & Ramalho-Santos, J., 2006. Effects of hyperglycemia on sperm and testicular cells of Goto-Kakizaki and streptozotocin-treated rat models for diabetes. Theriogenology, 66(9): 2056–2067.

Aybek, H., Aybek Z, Rota S, Sen N, Akbulut M, 2008. The effects of diabetes mellitus, age, and vitamin E on testicular oxidative stress, Fertil Sterile J., 90(3):755-80.

Ballester, J., Munoz M. C., Dominguez, J., Guinovart, J.J., Munoz, M.C., Rigau T., Rodriguez-Gil, J.E, 2004. Insulin-dependent diabetes affects testicular function by FSH- and LH-linked mechanisms. J Androl, 25(5): 706–719.

Bashandy, S.A.E., El-Awdan, S.A., Ebaid, H., Alhazza, I.M., 2016. Antioxidant Potential of Spirulina platensis Mitigates Oxidative Stress and Reprotoxicity Induced by Sodium Arsenite in Male Rats, Oxidative Medicine and Cellular Longevity, 2016: 1-8.

Bhat, V.B., Madyastha, K.M., 2000. C-phycocyanin: a potent peroxyl radical scavenger in vivo and in vitro. Biochem Biophy Res Comm, 275(1): 20–25.

Birben, E. ,Murat Sahiner, U., Sackesen, C., Erzurum, S., and Kalayci,O, 2012. Oxidative Stress and Antioxidant Defense.World Allergy Organ J, 5(1): 9–19.

Delfino M., Imbrogno, N., Elia J., Capogreco F, Mazzilli F., 2007. Prevalence of diabetes mellitus in male partners of infertile couples. Italy J Urol Nephrol, 59:131-135.

Deng, R., Chow, T.J., 2010, Hypolipidemic, Antioxidant and Antiinflamatory Activities of Microalgae Spirulina, Cardiovascular Therapeutics, 28: e33-e45.

El-Baz, F. K. Aly, H.F., El-Sayed, A.B., Mohamed, A.A., 2013, Role of Spirulina platensis in the control of glycemia in DM2 rats, International Journal of Scientific and Engineering Reasearch, 4 (12): 1-8.

El-Dosky G.E., Bashandy S.A., Alhazza I.M., Al-Othman Z., Mourad, Aboul-Soud M., Yusuf K., 2013. Improvement of Mercuric Chloride-Induced Testis Injuries and Sperm Quality Deteriorations by Spirulina platensis in Rats, PLOS One, 8(3): 1-9.

Elgawish, R., Abdelrazak, H. M. A., 2014, Effects of lead acetate on testicular function and caspase-3 expression with respect to the protective effect of cinnamon in albino rats, Toxicology Reports, 1(2014):795-801.

Firdiyani, F., Agustini, T.W., Ma’ruf, W.F., 2015. Ekstaksi Senyawa Bioaktif sebagai Antioksidan Alami Spirulina platensis Segar dengan Pelarut yang Berbeda, Jurnal Pengolahan Hasil Perikanan Indonesia, 18(1): 28-37.

Hanukoglu, I., 2006. Antioxidant protective Mechanisms against Reactive Oxygen Species (ROS) Generated by Mitochondrial P450 Systems in Steroidogenic Cells, Drug Metab Rev. 38(1-2), 171-96.

Haq, M., 2016. Pengaruh variasi konsentrasi pati ubi talas (Colocasia esculnta L.) sebagai Supending agent terhadap Sifat Fisik Suspensi Asam Mefenamat, Skripsi, Universitas Wahid Hasyim, Semarang.

International Diabetes Federation, 2003. Diabetes Atlas, Imprimerie L Vanmelle SA: Belgium.

Jiang, X., Chen, J., Zhang, C., Zhang, Z., Tan, Y., Feng, W., Skibba, M., Xin, Y., Cai, L., 2015, The Protective Effect of FGF21 on Diabetes-Induced Male Germ Cell Apoptosis Is Associated With Up-Regulated Testicular AKT and AMPK.Sirt1/PGC-1α Signaling, Endocrinology, 156 (3): 1156-1170

Jungwirth, A., Diemer T., Dohle GR., Kopa Z, Krausz C., Tournaye H., 2012, EAU Guidelines on Male Infertility, Eur Urol, 62 (2): 324-2, 226-241.

Karunakaran, U., and Park, K.G., 2013. A systematic review of oxidative stress and safety of antioxidants in diabetes: focus on islets and their defense, Diabetes Metab J, 37(2):106-12.

Khaki, A., Fathiazad, F., Nouri, M., Khaki, A., Maleki, N.A., Khamnei, H.J., Ahmadi, P., 2010. Beneficial effects of quercetin on sperm parameters in streptozotocin- induced diabetic male rats. Phytother Res, 24(9): 1285–1291.

Khaki, A.A., Hajhosseini, L.,Golzar, F.S., Ainechi, N., 2014. The Anti-oxidant Effect of Ginger and Cinnamon on Spermatogenesis Dysfunction of Diabetes Rats, African Journal of Traditional Alternative Medicine, 11(4), 1-8.

La Vignera, S., Condorelli, R., Vicari, E., D’Agata, R., Calogero, A. E., 2012. Diabetes mellitus and and sperm parameters. J Androl, 33(2):145-153.

Layam, A., Reddy, C.L.K., 2007. Antidiabetic Property of Spirulina, Diabetologia Croatia, 35-2, 29-33.

Mazo, V.K., Gmoshinshii, I.V., Zilova, I.S., 2004, Microalga spirulina in human nutrition, V. Opr. Pitan, 73 (1): 45-53.

Ozoguwu J. C., Obimba, K.C., Belonwu, C.D. & Unakalamba, C. B., 2013. The pathogeness and pathophysiology of type 1 and type 2 diabetes mellitus, Journal of Physiology and Pathophysiology, 4 (4): 46-57.

Pankaj, P. P., 2016. Cell Suspension of Spirulina platensis Partially Attenuates Alloxan Induced Alterations in Carbohydrate and Lipid Metabolism Diabetic Mice, IJPSR, 7(7): 2805-2812.

Papaccio G, Pisanti FA, Latronico MV, Ammendola E, Galdieri M. Multiple low-dose and single high-dose treatments with streptozotocin do not generate nitric oxide. J. Cell Biochem., 2000; 77(1):82-91.

Plutzky, J., 2011. Macrovascular effects and safety issues of therapies for type 2 diabetes, Am J Cardiol, 108(3): 25B–32B.

Rezvanfar, M.A., Sadrkhanlou, R. A., Ahmadi, A., Sadee,H.S., Rezvanfar, M.A., Mohammad, A., Salehnia, A., Abdollahi, M., 2008. Protection of cyclophosphamide-induced toxicity in reproductive tract histology, sperm characteristics, and DNA damage by an herbal source; evidence for role of free-radical toxic stress, Human and Experimental Toxicology, 27(12): 901–910.

Sacarano, W.R., Messias, A.G., Oliva, S.U., Klinefelter, G.R., Kempinas, W.G., 2006. Sexual behaviour, sperm quantity and quality after shortterm streptozotocin-induced hyperglycaemia in rats, Int. J. Androl. 29(4): 482–488.

Shazly, M.O., Ahmed, K.A., Adel-Mawla., E., 2015. Therapeutic Effect of Spirulina platensis on Streptozotocin-Induced Diabetes Rats, Egypt. J. Comp. Path &Clinic Path, 28(1): 18- 31.

Shrilatha B, Muralidhara, 2007. Early oxidative stress in testis and epididymal sperm in streptozotocin-induced diabetic mice: Its progression and genotoxic consequences, Reprod Toxicol, 23(4): 578-87.

Wang, L., Guo L., Zhang L., Zhou Y., He Q., Zhang Z., Wang M., 2013. Effects of Glucose Load and Nateglinide Intervention on Endothelial Function and Oxidative Stress, Journal of Diabetes Research, 2013: 1-9.

Widodo, F.T. 2009. Hubungan antara jumlah leukosit dengan motilitas sperma pada hasil analisa sperma pasien infertilitas di RSUP DR Kariadi Semarang. Laporan Akhir Penelitian. Universitas Diponegoro.

Won, H. N, Il, K. K., Hae, S.A., Mi, J.K, Kang, H.G, Jun, J.H., Gye, M.C., 2012. Effect of Spirulina maxima on spermatogenesis and steroidogenesis in streptozotocin-induced type I diabetic male rats, Food Chemistry, 134(1):173–179.

Yigit, F., Guerevin E.G., Basok B.I., Esener O.B.B., Keser O., 2016. Protective effect of Spirulina platensis against cell damage and apoptosis in hepatic tissue caused by high fat diet., Journal of Biochemical and Histochemistry, 91(3): 182-194.

Zheng, J., Inoguchi T., Sasaki, S., Maeda, Y., McCarty, M.F., Fujii, M., Ikeda, N., Kobayashi, K., Sonoda, N., Takayanagi, R., 2013, Phycocyanin and Phycocyanobilin from Spirulina platensis protect against Diabetic Nephropathy by Inhibiting Oxidative Stress, Am J Regul Integr Comp Physiol, 304: R110-R120.

DOI: http://dx.doi.org/10.12928/pharmaciana.v9i1.9983

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