The performance of derivate FTIR spectrophotometry method compared to colorimetry for tranexamic acid tablet content determination

Ilma Nugrahani, Winni Nur Aulia


Recently, FTIR reported has been established as a direct content determination for some tablet dosage forms. In the method, infrared transmittance spectra was converted into the derivated absorbance form. In this present research, the method’s performance was investigated to quantified tranexamic acid in its tablet dosage form directly. The result then was compared to the colorimetry, which has been developed by another researcher. Correlations between the two methods were analyzed using t-test. The good linearity was shown at concentration range of 0.5 - 1.75% w/w at the wave number of NH group. Furthermore, the recovery, intra- and inter-day precision also fulfilled the validation requirement. Meanwhile, LOD and LOQ of the method were 0.0531%w/w and 0.1770%w/w. These methods were compared with colorimetry has been established before. Afterwards, the t-test proved no significant difference of content determination yielded, between these two methods. In conclusion, FTIR can be used for quantify the content of tranexamic tablet, more accurately than colorimetry, which has been developed before. Moreover, FTIR method also has advantages such as easier, simpler, faster and cheaper than the colorimetry method.  


Keywords: FTIR; quantitative; derivate spectrum, tranexamic acid; colorimetry

Full Text:



Ahuja S, Dong M, 2005, Handbook of Pharmaceutical Analysis by HPLC, Elsevier Academic Press, North Carolina, 191-200.

Ansari TM, Raza A, Rehman AU, 2005, Spectrophotometric determination of tranexamic acid in pharmaceutical bulk and dosage form, Anal Sci, 1133-1135.

Arayne MS, Sultana N, Shiddiqui FA, Mirza, AZ, Zuberi, MH, 2008, Spectrophotometric techniques to determine tranexamic acid: Kinetic studies using ninhydrin and direct measuring using ferric chloride, J Mol Struct, 891, 475–480.

British Pharmacopoeia, 2009, British Pharmacopoeia Volume IV, Medicines and Healthcare Products Regulatory Agency (MHRA), London ;10185.

Departemen Kesehatan Republik Indonesia, 1995, Farmakope Indonesia edisi IV, Departemen Kesehatan Republik Indonesia, Jakarta, 74.

ICH, Harmonised Tripartite Guideline, 2005, Validation of Analytical Procedures: Text and Methodology, Geneva, IFPMA.

Najiyya A, 2015, Pengembangan dan Validasi Metode Penetapan Concentration Ketokonazol Menggunakan FTIR, skripsi sarjana, Institut Teknologi Bandung, 1-7.

Neal MJ, 2006, At a Glance FARMAKOLOGI MEDIS, Fifth ed. Jakarta: Penerbit Erlangga; 71.

Nugrahani I, Kirana C, 2016, Determination of thiamine HCl (vitamin b1) and pyridoxine HCl (vitamin b6) content in tablet by FTIR, Int J Appl Pharm, 8, 257-264.

Nugrahani I, Musaddah M., 2016, Development and validation analysis of acyclovir tablet content determination method using FTIR. Int J Appl Pharm, 8, 43-47.

Refat MS, Mohamed GG, Fathi A, 2010, Spectrophotometric Determination of Allopurinol Drug in Tablets: Spectroscopic Characterization of the Solid CT Complexes, Bull Korean Chem Soc, 31(6): 1535-1542.

Riyanto, 2014, Validasi and Verifikasi Metode Uji Sesuai dengan ISO/IEC 17025 Laboratorium Pengujian dan Kalibrasi, Deepublish, Yogyakarta, 20-63.

Skoog DA, West DM, Holler FJ, 2007, Principles of Instrumenal Analysis 6th edition.

Belmont: Thomson Higher Education, 430-480.

Smith BC, 1996, Fundamental Fourier Transform Infrared Spectroscopy, US: CRC Press, 139.

Sweetman SC, 2009, Martindale : The Complete Drug Reference 36th Edition, Chicago : Pharmaceutical Press, 552, 1080.

Tampubolon HB, Sumarlik E, Mochammad Y, Gunawan I, 2005, Densitometric Determination of Tranexamic Acid in Tablets: Validation of the Method, J Liq Chromatogr Related Technol, 28(20), 3243 – 3254.

Teli MS, Shukla S, Sawant SS, 2015, Estimation of Tranexamic Acid in Its Tablet and Bulk Formulation by RP-HPLC Method, World J Pharm Pharm Sci, 4(12), 1404-1415.



  • There are currently no refbacks.

Copyright (c) 2018 Pharmaciana

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.


Pharmaciana indexed by:

Google ScholarDirectory of Open Access JournalIndonesian Scientific Journal Database (ISJD)Indonesian Publication Index (IPI)CrossrefBielefeld Academic Search Engine (BASE)Sherpa RomeoOpen Academic Journal Index (OAJI)





Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.