VALIDASI METODE ANALISA PENETAPAN KADAR EPIGALOKATEKIN GALAT DENGAN KLT DENSITOMETRI
Abstract
TLC Densitometry is one of method which was used to measure amount of active
substance. Active substance of epigallocathechin gallate is one of in green tea extract
cream. Method of analysis must be validated to prove that method will give the data
close with the real value. Aim of this research is to prove that TLC Densitometry method
have liniearity, precision and accuration that fulfill the requirement. This study used
concentration 600, 1800 and 3000 ug/ml with 3 replications to measure precision
(based on value of CV) and accuration (based on value of recovery). Liniearity was
known based on value of r of curve regression between concentration and wide area of
chromatogram. LOD and LOQ was calculated based on curve regression. Research
showed that method of analyse had liniearity with r= 0,98. Value of CV concentration
of 600, 1800 and 3000 ug/ml were 8,18%; 3,19% and 1,53%, respectively and recovery
were 88,10%; 99,65% dan 111,33%, respectively. Value of LOD was 827,01 µg/ml and
LOQ was 2756,69 µg/ml.
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PDFDOI: http://dx.doi.org/10.12928/pharmaciana.v2i1.656
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Copyright (c) 2021 Nining Sugihartini, Achmad Fudholi, Suwidjiyo Pramono, Sismindari Sismindari
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