Chloroform fraction of ethanolic extract of Elephantopus scaber Linn. increase the p53 expression on human breast cancer (T47D) cell line

Nurkhasanah Mahfudh, Nanik Sulistyani, Lukman Mahdi


Elephantopus scaber Linn had been used as traditional medicine. It contain some lacton sesquiterpene with antiproliferative activity. The study aim was to explore the effect of chloroform fraction of ethanolic extract of E.scaber on the expression of p53 on T47D cell line. The ethanol extract was found by maceration method. The extract was then fractionated using petroleum ether and chloroform respectively to get the chloroform fraction. The expression of p53 was observed by immunocytochemistry method with antip53 antibody. The p53 cell line was cultured and treated with fraction with concentration of 7.06 and 3.53 µg/ml. The expression of p53 was showed by brown colour in the nucleus of the cell. The result showed that chloroform fraction treatment with concentration of 7.06 µg/ml induced apoptotic cell death in the end stage (necrosis). While the treatment with lower concentration 3.53 µg/ml could increase the p53 expression.


Elephantopus scaber, chloroform fraction, T47D cell line, p53 expression

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Berghe, T. Vanden, Grootjans, S., Goossens, V., Dondelinger, Y., Krysko, D. V, Takahashi, N., Vandenabeele, P., 2013. Determination of apoptotic and necrotic cell death in vitro and in vivo. Methods 3, 1–13.

Bosari, S., Lee, A.K.C., Viale, G., Hcatley, G.J., Coggi, G., 1992. Abnormal p53 immunoreactivity and prognosis in node-negative breast carcinomas with long-term follow-up. Virchows Arch. A Pathol. Anat. Histopathol. 421, 291–295.

Chaturvedi, D., 2011. 10 . Sesquiterpene lactones : Structural diversity and their biological activities. Res. Signpost 661, 313–334.

Geetha, B.S., Nair, M.S., Latha, P.G., Remani, P., 2012. Sesquiterpene Lactones Isolated from Elephantopus scaber L . Inhibits Human Lymphocyte Proliferation and the Growth of Tumour Cell Lines and Induces Apoptosis In Vitro. J. Biomed. Biotechnol. 2012, 1–7.

Ho, W.Y., Yeap, S.K., Ho, C.L., Raha, A.R., Suraini, A.A., Alitheen, N.B., 2011. Elephantopus scaber induces cytotoxicity in MCF-7 human breast cancer cells via p53-induced apoptosis. J. Med. Plants Res. 5, 5741–5749.

Kabeer, F.A., Sreedevi, G.B., Nair, M.S., 2014. Isodeoxyelephantopin from Elephantopus scaber ( Didancao ) induces cell cycle arrest and caspase-3-mediated apoptosis in breast carcinoma T47D cells and lung carcinoma. Chin. Med. 9, 1–9.

Listyowati, Y., Nurkhasanah, 2014. Efek sitotoksik dan pemacuan apoptosis fraksi petroleum eter ekstrak etanol daun tapak liman ( Elephantopus scaber linn ) terhadap sel Hela. Pharmaciana 3, 1–7.

Malkin, D., 2001. The role of p53 in human cancer. J. Neuro Oncol. 51, 231–243.

Nurkhasanah, Trisnamurti, K.C., Gunaryanti, R.D., Widyastuti, T., 2015. The Screening Of Cytotoxic Fraction From Elephantopus scaber Linn against Human Cervical Cancer ( Hela ) Cells. Int. J. Pharma Sci. Res. 6, 1011–1014.

Silva, M.T., 2010. Secondary necrosis : The natural outcome of the complete apoptotic program. FEBS Lett. 584, 4491–4499.

Silva, M.T., Vale, A. do, Santos, M.N.N., 2008. Secondary necrosis in multicellular animals : an outcome of apoptosis with pathogenic implications. Apoptosis 13, 463–482.

Skommer, J., Wlodkowic, D., Deptala, A., 2007. Larger than life : Mitochondria and the Bcl-2 family. Leuk. Res. 31, 277–286.

Stop Kanker, 2015. . Pusat Data dan Informasi Kemenkes RI, Jakarta.

Suyanto, Y.P., Utomo, R.A., Sandra, F., 2008. p53 dan cancer payudara. Indones. J. Cancer 4, 138–143.

Vousden, K.H., Lu, X., 2002. Live or let die: the cell’s response to p53. Nature 2, 594–604.



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