PENETAPAN KADAR FENOLIK TOTAL EKSTRAK METANOL KELOPAK BUNGA ROSELLA MERAH (Hibiscus sabdariffa Linn) DENGAN VARIASI TEMPAT TUMBUH SECARA SPEKTROFOTOMETRI

Abstract

The purpose of this research is to determine the total phenolic content of Hibiscus
sabdariffa calyx in variations of growing area. Red Rosell calyxs were collected from
Glagah, Kediri, and Samigaluh. Phenolic compounds of Hibiscus sabdariffa calyx were
extracted using maceration method with methanol. Total phenolics content were
determined using visible spectrophotometry method with Folin Ciocalteau reagent. The
principle of this method is the formation of blue complex compound from
phospomolybdate-phosphotungstate reduced by phenolic compound in the basic
condition, which can be measured by spectrophotometry. Galic acid was used as
comperator in this research. Total phenolic content in red calyx Glagah, Kediri and
Samigaluh were respectively 1.40 g GAE/100 g extract, SD 0.06 (n=12), 1.41 g
GAE/100 g extract, SD 0.07 (n=12) dan 2.12 g GAE/100 g extract, SD 0.05 (n=15).
Based on this results it could be concluded that growing area affected total phenolic
content in the methanol extract of red calyx Rosell.