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Application method of Fourier Transform Infrared (FTIR) combined with chemometrics for analysis of rat meat (Rattus Diardi) in meatballs beef
Abstract
Food product forgery begins to become a new problem in Indonesia, the main problem is there is a concern that food product is mixed with forbidden meat, one of may is rat meat in meatball. This study is expected to become one basic in order to meet meatball authenticity guarantee.
Study about the utilization of infrared spectrophotometry Fourier Transform (FTIR) which was combined with chemometric was carried out to identified rat meat in meatball quantitative and qualitatively. Concentration variation of beef and rat meat in producing meatball is 0%, 25%, 35%, 65%, 75% and 100% rat meat in meatball dough and 5 samples of market meatball. Beef and rat fat which were contained in meatball was extracted by Soxhlet in temperature ≤ 70oC for 7 hours with n-hexane solvent.
Qualitative and quantitative analysis was carried out in wave range 750-1600 cm-1 by using partial least square (PLS) multivariate analysis technique and principal component analysis (PCA). The result of calibration with y = 0,9720x + 1,580, coefficient of determination (R2) = 0,9941, and root mean square error of calibration (RMSEC) value was 1.63%. Validation model with, mean root mean square error of cross validation (RMSECV) value was 1.79%, and root mean square error of prediction (RMSEP) value was 2.60%. Using Principal Component Analysis (PCA), grouping of cow fat and mouse fat in the meatballs successfully.
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Che Man, Y.B., Syahariza, Z.A., Mirghani, M.E.S., Jinap, S., and Bakar, J., 2005, Analysis of potential lard adulteration in chocolate and chocolate products using Fourier transform infrared spectroscopy. Food Chemistry, 90:815-819.
Che Man, Y.B., Syahariza, Z.A., and Rohman A., 2010, Chapter 1. Fourier Transform Infrared (FTIR) Spectroscopy: Development. Tehnique, and Application in the Analysis of Fats and Oils, in Fourier Transform Infrared Spectroscopy edited by Oliver J Ress, Nova Science Publisher, New York.
Coltro, W. K. T., Ferreira, M. M. C., Macedo, F. A. F., Oliveira, C. C., Visentainer, J. V., Souza, N. E., and Matsushita, M., 2005, Correlation of Animal Diet and Fatty Acid Content in Young Goat Meat by Gas Chromatography and Chemometrics, Meat Science, 71:358-363.
Guillén, M.D. and Cabo, N., 1997, Characterization of edible oils and lard by fourier transform infrared spectroscopy. Relationships between composition and frequency of concrete bands in the fingerprint region. Journal of the American Oil Chemists’ Society, 74:1281-1286.
Huda, N, Shen, Y.H, Huey, Y.L, Ahmad, R. dan Mardian, A, 2009, Evaluation of Physico-chemical Properties of Malaysian Commercial Beef Meatball, American Journal of Food Technology.
Julaikah, N., 2013, Harga daging sapi Indonesia salah satu yang termahal di dunia, http://www.merdeka.com/uang/harga-daging-sapi-indonesia-salah-satu-yang-termahal-di-dunia.html, 5 September 2014.
Maryam, S., Sismindari; Raharjo, T.J; Sudjadi; and Rohman, A., 2016, Analysis of porcine contamination in dendeng using mitochondrial D-loop 686 and cyt b gene primers by real time polymerase chain reaction. International Journal of Food Properties, 19:187-195.
Miller, J.N., and Miller, J.C., 2005, Statistics and Chemometrics for Analytical Chemistry, 4 th Edition, , Pearson Education Limited, Harlow, pp 232-235
Miller, J.N., and Miller, J.C., 2010, Statistic and Chemometrics for Analytical Chemist, 6th Edition, Pearson Education Limited, Harlow, pp 89-105.
Mursyidi, A., 2013, The Role of Chemical Analysis in the Halal Authentication of Food and Pharmaceutical Products. Journal of Food and Pharmaceutical Sciences, 1:76-86.
Purnomo, H. and Rahardiyan, D., 2008, Review Article: Indonesian Traditional Meatball. International Food Research Journal, 15:101-108.
Rahmawati, Sismindari, Tri Joko Raharjo, Sudjadi, Abdul Rohman, 2016, Analysis of pork contamination in Abon using Mitochondrial DLoop22 Primers using Real Time Polymerase Chain Reaction Method. International Food Research Journal, 23(1):370-374.
Rohman, A. and Che Man, Y., 2010a, FTIR spectroscopy combined with chemometrics for analysis of lard in the mixtures with body fats of lamb, cow, and chicken. International Food Research Journal, 17:519-526.
Rohman, A. and Man, Y.B.C., 2010b, Fourier transform infrared (FTIR) spectroscopy for analysis of extra virgin olive oil adulterated with palm oil. Food Research International, 43:886-892.
Rohman, A., Erwanto, Y., & Man, Y. B. C., 2011, Analysis of pork adulteration in beef meatball using Fourier transform infrared (FTIR) spectroscopy. Meat Science, 88:91-95.
Rohman, A., Triyana, K., Sismindari, and Erwanto, Y., 2012, Differentiation of lard and other animal fats based on triacylglycerols composition and principal component analysis. International Food Research Journal, 19(2):475-479.
Schieber, A., 2008, Introduction to Food authentication. In D-W. Sun (Ed.), Modern techniques for food authentication, Elsevier Inc, New York, pp 1-23.
Sudjadi, H.S. Wardani, T. Sepminarti and Rohman, A., 2016, Analysis of porcine gelatin DNA in commercial capsule shell using real-time polymerase chain reaction for halal authentication. International Journal of Food Properties doi: 10.1080/10942912.2015.1110164.
Syahariza, Z.A., Man, Y.B.C., Selamat, J., and Bakar, J., 2005, Detection of lard adulteration in cake formulation by Fourier transform infrared (FTIR) spectroscopy. Food Chemistry, 92:365-371.
Van der Spiegel, M., van der Fels-Klerx, H.J., Sterrenburg, P., van Ruth, S.M., Scholtens-Toma, I.M.J., and Kok, E.J., 2012, Halal assurance in food supply chains: Verification of halal certificates using audits and laboratory analysis. Trends in Food Science & Technology, 27:109-119.
DOI: http://dx.doi.org/10.12928/pharmaciana.v7i2.4247
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