ISOLASI DAN UJI PENANGKAPAN RADIKAL BEBAS DPPH OLEH ISOLAT-1, FRAKSI ETIL ASETAT, DAN EKSTRAK ETANOL AKAR PASAK BUMI (Eurycoma longifolia Jack)

Abstract

The free radical was produced when undertaking processes metabolit or opposed
infection and when the body dissolved food. The unstable free radical could be
neutralised with antioxidant. The root of the pasak bumi (Eurycoma longifolia Jack)
was known contained the compound flavonoid, that it was known had the activity as
antioxidant. This research aimed at knowing the activity capacity of the capture of the
free radical the water fraction and the faction ethyl acetate fraction the extract ethanol
of the root of the pasak bumi by using the DPPH method.
The root of the pasak bumi was extracted with ethanol 70% used maceration. The
ethanolic extract was dissolved in ethyl acetate, fractination with ethyl acetate so as to
be received by the fraction ethyl acetate. The concentration of the ethanolic extract and
the fraction of ethyl acetate that was used were 2; 4; 8; 16 ug/mL and isolat-1 that is:
0.8; 1.6; 3.2; and 6.4 ug/mL.
Results of the research showed that all the treatments had the activity as
scavenging the free radical. Results of the analysis statistika with the Kruskal Wallis
method in the level of the confident 95% that was followed by the test Mann Whitney
showed the existence of the difference of the activity of the capture of the free radical
who was significant between respectively the treatment group. The conclusion from this
research was the value ES50 ethanalic extract (15.64 ug/mL) bigger than the faction
ethyl acetate fraction and higher (13.948 ug/mL) value of ethanalic extract was than
isolat 1 (3.961 g/mL).