KERAGAMAN ISOLAT ACTINOMYCETES BERDASARKAN ANALISIS RFLP TERHADAP GEN NRPS

Abstract

Actinomycetes is one of the types of microorganisms that is essential for
producing the active compounds, one of which is an antibiotic. Isolation of
Actinomycetes from the rhizosphere of rice plant (Oryza sativa L.) and tin plant (Ficus
carica L.) has yielded 13 isolates of antibiotic-producing Actinomycetes. This study
aims to determine the diversity of isolates by RFLP analysis (Restriction Fragment
Length Polymorphism) of the NRPS (Non Ribosomal Peptide Synthetase) gene. The
study was carried out by isolating Actinomycetes isolate DNA and performed PCR on
the 16SrRNA and NRPS genes. The RFLP analysis of the NRPS gene PCR products is
then performed using the enzyme HaeIII. Identify the presence of DNA, the PCR
product and RFLP results was performed by agarose gel electrophoresis. The diversity
of isolates was analyzed by multivariate analysis. The results showed that the diversity
of 13 isolates of Actinomycetes can be grouped into 5 groups: isolates 5 (group 1),
isolates 8 (group 2), isolates 11 (group 3), isolates 6,7,9 (group 4) and isolates
1,2,3,4,10,12,13 (group 5).