BIOAVAILABILITAS TABLET IBUPROFEN PADA PEMBERIAN BERSAMAAN DENGAN EKSTRAK AIR HERBA PEGAGAN (Centella asiatica (L) Urban) PADA KELINCI JANTAN

Abstract

The tendency to use herbal drug simultaneously with synthetic drug may result in
an interaction. Based on research, aqueous extract of Centella asiatica (L) Urban
contains saponin (asiaticoside and madecassoside) compounds that inhibit CYP450
and is a safe herbal alternative that is used for anti-inflammatory treatment. The aim of
this study was to investigate the effect of aqueous extract of Centella asiatica (L) Urban
to the ibuprofen tablet bioavailability in male rabbits. Both compounds are metabolized
by CYP450 enzymes in the same way with different mechanism. The cross over design
with the same subject was used in this study. The samples consisted of 400 mg ibuprofen
with 0%b/v, 25%b/v, 50%b/v dan 100%b/v aqueous extract of Centella asiatica (L)
Urban respectively. The blood were taken after 0; 0,5; 1; 1,5; 2; 2,5; 3; 3,5; 4; 6; 8; 10
hours after samples treatment, then the ibuprofen plasma concentration were
determined spectrophotometrically. The results then were used to evaluat the
bioavailability parameters: tmaks, Cpmaks, and AUC and were analyzed using ANOVA
test in the same way as well as the 95% trust standart. The results showed, the use of
ibuprofen tablet stimultaneously with aqueous extract of Centella asiatica (L) Urban
increased Cpmaks dan AUC, but there were no influence to the tmaks value. The use of
ibuprofen tablet stimultaneously with 50 %b/v and 100 %b/v aqueous extract of
Centella asiatica (L) Urban had a significant influence to the bioavailability of
ibuprofen.