STUDI PENETAPAN KADAR LOSARTAN DENGAN METODE SPEKTROFOTOMETRI DAN HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) SERTA APLIKASINYA PADA TRANSPOR TRANSDERMAL in vitro

Abstract

The development of drug delivery system needs a usefull determination method of
drug in many kind of samples. This research was purposed to develop a determination
method of losartan, an angiotension receptor antagonist II, from the sample of in vitro
transdermal transport. Three methods were studied, i.e. normal spectrophotometric, 1st
derivative spectrophotometric, and HPLC. The spectrophotometric method was
conducted using Spectrophotometer Shimadzu tipe UV 1700 controlled by UV Probe
software (Shimadzu), while HPLC method was performanced by Shimadzu HPLC
controlled by LC Solution software (Shimadzu). Lichrospher RP 18 250-4 (5 µm) was
used as stationary phase and acetonitril-acetic buffer 0,01 M pH 4 (60:40) was used as
mobile phase. Chromatogram was recorded using UV 223 nm and 254 nm as a
detector. Some parameters of determination method performance were calculated, i.e.
LOD, LOQ, recovery, systemic error, and random error. The results shown that
spectrofotometric methods did not have an enough performance parameters to use in
transdermal transport of losartan, while HPLC method had LOD and LOQ 27,329 and
91,098 ng/ml using UV 223 nm as detector and 36,178 and 120,590 ng/ml using UV 254
nm as detector. Detector UV 223 nm was selected. This HPLC method had recovery
106,405% and random error 3,71%, and could be used to determine the losartan
concentration in sample from transdermal transport in vitro.