STANDARISASI PARAMETER NON SPESIFIK DAN PERBANDINGAN KADAR KURKUMIN EKSTRAK ETANOL DAN EKSTRAK TERPURIFIKASI RIMPANG KUNYIT

Barokati Azizah, Nina Salamah

Abstract


Turmeric (Curcuma domestica Val.) is one of the plants that contain curcumin
compounds with various activity. Use of curcumin from turmeric is widely used in the
form of ethanolic extract, but there are ballast substances that cause low levels of
curcumin. This can be pursued with the manufacture of a purified of ethanolic extract.
Ethanolic extract of turmeric was made by maceration using ethanolic 96%. Ethanolic
extract soaked with hexane until the hexane phase is clear and purified extract obtained
is insoluble hexane phase. Curcumin levels determined using Thin Layer
Chromatography (TLC) method with the stationary phase silica gel 60 F and mobile
phase of chloroform: ethanolic: glacial acetic acid (94: 5:1) and be analyzed
quantitatively using densitometry with a maximum wavelength of 426 nm . The water
content of the extract determined using toluene distillation method, ash and acid
insoluble ash content determined using gravimetric method. The statistic analysis with
LSD showed significant differences of curcumin content and analysis of non-specific
parameter values on ethanolic extract and purified extract.


Keywords


Curcuma domestica; curcumin; purified extract; non-specific parameter

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DOI: http://dx.doi.org/10.12928/pharmaciana.v3i1.416

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Pharmaciana
ISSN Print: 2088-4559 | ISSN Online: 2477-0256
Website: http://journal.uad.ac.id/index.php/PHARMACIANA
Office: Faculty of  Pharmacy, Universitas Ahmad Dahlan
Jl. Prof. Dr. Soepomo, S.H., Janturan, Warungboto, Umbulharjo, Yogyakarta, Indonesia
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Email: pharmaciana@pharm.uad.ac.id