EFEK FRAKSI ETIL ASETAT EKSTRAK ETANOL AKAR PASAK BUMI (Eurycoma longifolia, Jack) TERHADAP AKTIVITAS FAGOSITOSIS MAKROFAG SECARA IN VITRO

Aminda Nur Arifah, Nurkhasanah Nurkhasanah

Abstract


Eurycoma longifolia has been used as a remedy for detoxification, aphrodisiaca, anticancer and
immunistimulant. The quassinoids of pasak bumi root extract, have antioxidant activity and
immunomodulatory activity by increasing IL-12. This study aims to determine the effect of the ethyl
acetate fraction of ethanolic extract of pasak bumi root on macrophages phagocytic activity in vitro.
Macrophages were isolated from peritoneal fluid of male Balb/Cmice, and thenwere cultured for 24
hours in 24 well plate. The ethyl actetate fraction ethanolic extract of pasak bumi root with
concentration of 10, 50, and 100 µg/ml, lipopolysaccharides and dimethyl sulfoxide were added, and
incubated for 1.5 hours. The phagocytosis tests was carried out by adding latex with density
5x104
/well and incubated for 1.5 hours at incubator (5% CO2,37o
C). Macrophage were then stained by
Giemsa. The results showed that the percentage of active phagocytic cells treated with ethyl actetate
fraction of ethanolic extract of pasak bumi root of 10, 50 and 100 µg/ml, lipopolysaccharides, and
dimethyl sulfoxide were 95.4%, 90%, 85.4%, 83.4%, and 79% respectively. Phagocytic capacity at
treatment levels of 10, 50, and 100 µg/ml, lipopolysaccharides,and dimethyl sulfoxide were 469; 439.8; 360.8; 204.6; and 147.6 respectively. The result showed thatreatment of ethyl acetate fraction
of ethanolic extract of pasak bumi root can increase the phagocytic activity of macrophages in vitro.


Keywords


ethyl acetate fraction; Pasak bumi root; macrophage phagocytic activity; immunomodulator

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DOI: http://dx.doi.org/10.12928/pharmaciana.v4i1.392

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Pharmaciana
ISSN Print: 2088-4559 | ISSN Online: 2477-0256
Website: http://journal.uad.ac.id/index.php/PHARMACIANA
Office: Faculty of  Pharmacy, Universitas Ahmad Dahlan
Jl. Prof. Dr. Soepomo, S.H., Janturan, Warungboto, Umbulharjo, Yogyakarta, Indonesia
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Email: pharmaciana@pharm.uad.ac.id