UJI AKTIVITAS ANTIOKSIDAN FRAKSI ETER HASIL HIDROLISIS INFUSA DAUN BINAHONG (Anredera cordifolia (Ten.) Steenis) DENGAN METODE DPPH (1,1-DIPHENIL-2-PICRYLHYDRAZYL)

Abstract

Antioxidant is compounds which have abilities to inhibit oxidation rate. Anredera cordifolia
folium consist of flavonoid which have activities as antioxidant. The purpose of this research was to
know antioxidant activity at ether fraction of hydrolysed aquous extract of Binahong folium (Anredera
cordifolia). Ether fraction was obtained by hydrolysing the infuse Binahong folium (Anredera
cordifolia) with HCl 2N then fractionated with ether and then evaporated. The antioxidant activity was
measured by DPPH method using spectrophotometer. The maximum wavelength and operating time
quercetin and samples were 515,0 nm (quersetin) and 514,0 nm (ether fraction), 19-30 minute
(quersetin) and 19-29 minute (ether fraction). Scavenging activity test done with the DPPH method
(1,1- dyphenyl-2-picrylhydrazyl). The decreasing of DPPH radical solution decreased the absorbant
intensity. The percentage of radical scavenging activity was performed as EC50 (Effective
Concentration). The results indicated that both samples showed free radical scavenging activity with
EC50 value for quercetin (2,702±0,15) µg/ml, and ether fraction of (249,31±9,26) µg/ml. Statistical
analysis using Kruskall Wallis method with 95% of confident level followed by Mann Whitney test

giving evidence the results between samples and positive control (quercetin) are significantly
difference.