Antibacterial activity of dialkyl-aginate biosurfactant cream againts Staphylococcus aureus an Pseudomonas aerugynosa

Kintoko Kintoko, Nining Sugihartini, Benazir Evita Rukaya

Abstract


Dialkyl-alginate biosurfactant is an amphifilik rhamnolipid biosurfactant that has the potential to be developed into an antibacterial agent. The purpose of this study was to prove that the biosurfactant of dialkyl alginate both before and after creams has antibacterial activity especially against Staphylococcus aureus and Pseudomonas aeruginosa. Test of antibacterial activity of biosurfactant dialkyl alginate at concentrations of 5%, 10% and 20% to Staphylococcus aureus and Pseudomonas aeruginosa using Hammond, et al (2011) modified quantitative method. Enbatic® 1% is used as a positive control and sterile aquadest as a negative control. Determination of antibacterial activity of dialkyl alginate biosurfactant followed by analysis of leakage of protein and nucleic acids using UV-vis Spectrophotometry and leakage of Ca2 + and K + metal ions using Atomic Absorption Spetrophotometry (AAS). The most active concentration was formulated into cream and then performed physical evaluation (organoleptic, pH, adhesion, spreading and viscosity) and tested antibacterial activity using the well method. The test results showed that the biosurfactants of dialkyl-alginate before and after the cream was treated as antibacterial activity. The concentration of 10% was the most active concentration having activity which did not differ significantly to positive control with p value of 0,05

Keywords


Antibacterial activity; Alginate; Biosurfactant; Cell leak; Sargassum sp

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References


Agusta, A. Jamal, Y., Irawati, P., and Fathoni, A., 2013. Chemical constituents and antibacterial effect of essential oil of javaneese pepper leaves (Piper retrofractum Vahl., Media Litbangkes, 23(2): 65-72.

Ahmad, B., Khan, F., Ahmed, J., Cha, S.B., Shin, MK., Bashir, S., Yoo, H.S., 2014. Antibiotic resistance pattern and molecular epidemiology of methicillin-resistant Staphylococcus aureus colonization in burns unit of a tertiary care hospital in Peshawar, Pakistan, Tropical Journal of Pharmaceutical Research, 13(12): 2091-2099.

Arino, S.; Marchal, R.; Vandecasteele, J.P., 1998. Involvement of a rhamnolipid-producing strain of Pseudomonas aeruginosa in the degradation of polycyclic aromatic hydrocarbons by a bacterial community. J. Appl. Microbio 84: 769-776.

Azrifitria, Azis, S., Chairul, 2010. Aktivitas antibakteri ekstrak etanolik daun dan umbi Crinum asiaticum L. terhadap bakteri penyebab jerawat, Journal, Majalah Farmasi Indonesia, 2(4): 249-254.

Bunduki, M.M.C., Flanders K.J., Donnelly C.W., 1995. Metabolic and structural site of damage in heat and sanitizer-injuried population of Listeria monocytogenes. Journal Food Protect, 58: 410-415.

El-Sheshtawy, H.S. and Doheim, M.M., 2014, Selection of Pseudomonas aeruginosa for biosurfactant production and studies of its antimicrobial activity, Egyptian Journal of Petroleum, 23: 1-6.

Estiasih, T. dan Ahmadi K., 2009. Teknologi Pengolahan Pangan. Bumi Aksara. Jakarta.

European Committee for Antimicrobial Susceptibility Testing (EUCAST), 2015, Disk diffusion method, Clinical Microbiology and Infection, Version 5.0.

Hammond, A.A., Miller, K.G., Kruczek, C.J., Dertien, J., Colmer-Hammood, J.A., Griswold, J.A., Harswill, A.R., Hamood, A.N., 2011. An in vitro biofilm biofilm model to examine the effect of antibiotic ointments on biofilms produced by burn wound bacterial isolates, NIH Public Acces, 37(2): 2-3.

Handayani, T.S., 2016. Pola kuman dan resistensinya terhadap antibiotic pada penderita gangren diabetic di Rumah Sakit X bulan februari-maret 2016, Skripsi, Universitas Muhammadiyah Surakarta.

Harsini, H., 2017. Aktivitas antibakteri ekstrak etanolik kulit batang jambu mete (Anacardium occidentale Linn.) terhadap Staphylococcus aureus.Majalah Kedokteran Gigi Indonesia 3(3): 128-132.

He, W., Kaniga, K., Lynch, A.S., Flamm, R.K., Davies, T.A., 2012. In vitro Etest synergy of doripenem with amikacin, colistin, and levofloxacin againt Pseudomonas aeruginosa with defined carbapenem resistance mechanism as determined by the Etest method, Elsevier Journal, 74(4): 417-419.

Irfan, 2015. In vitro synergistic effect of biosurfactant produced by Bacillus substilis MTCC 441 against drug resistant Staphylococcus aureus, Journal of Applied Pharmaceutical Science, 5(03): 113-116.

Jamal, Y., Irawati, P., Fathoni, A., and Agusta, A., 2013. Chemical constituents and antibacterial effect of essential oil of javaneese pepper leaves (Piper retrofractum Vahl., Media Litbangkes, 23(2): 65-72.

Mahreni dan Reningtyas, R., 2015. Pembuatan surfaktan dialkil karbohidrat dari alga, Prosiding Seminar Nasional Teknik Kimia “Kejuanganâ€, B-18: 1-7.

Ndlovu, T., Rautenbach, M., Vosloo, J.A., Khan, S., Khan, W., 2017. Characterisation and antimicrobial activity of biosurfactant extracts produced by Bacillus amyloliquefaciens and Pseudomonas aeruginosa isolated from a wastewater treatment plant, AMB Express (Springer Open), 7: 1-19.

Susilowati, E.P. dan Wahyuningsih, S.S., 2014. Optimasi sediaan salep yang mengandung eugenol dari isolasi minyak cengkeh (Eugenia caryophylatta Thumb.), Indonesian Journal On Medical Science, 1(2): 29-34.

Sotirova, A., Spasova, D., Vasileva-Tonkova, E., Galabova, D., 2009. Effects of rhamnolipid-biosurfactant on cell surface of Pseudomonas aeruginosa. Microbiol. Research 164: 297-303.

Syarifuddin, A., 2017, Penapisan senyawa antibiotik isolat bakteri dari rizofer kayu putih (isolat KP 13) dan analisa kebocoran sel, Tesis, Universitas Ahmad Dahlan, Yogyakarta.

Ulaen, S. P.J., Banne, Y., Suatan, Ririn, A., 2012. Pembuatan salep anti jerawat dari ekstrak rimpang temulawak (Curcuma xanthorrhiza Roxb.), Jurnal Ilmiah Farmasi, 3(20): 45-49.

Vasileva-Tonkova, E., Sotirova, A., Galabova, D., 2010. The effect of rhamnolipid biosurfactant produced by Pseudomonas fluorescens on model bacterial strains and isolates from industrial wastewater. Curr. Microbiol.62(2): 427-433.

Zakaria, A.S., Afifi, S.A., Elkhodairy, K.A., 2016. Newly developed topical cefotaxime sodium hydrogels: antibacterial activity and in vivo evaluation, Journal of Biomedical Research International, 2016: 1-15.




DOI: http://dx.doi.org/10.12928/pharmaciana.v8i2.10337

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