Antioxidant-phenolic content correlation of phenolics rich fractions from Dillenia suffruticosa wood bark

Rini Muharini, Ira Lestari, Masriani Masriani

Abstract


Dillenia suffruticosa is one of the tropical medicinal plants that has been used traditionally for treating several tropical diseases. However, phytochemical and pharmacological investigations of D. suffruticosa wood bark have not been well-investigated. This research aimed to investigate the correlation between antioxidant properties and total phenolic content of phenolics rich extract and fractions from D. suffruticosa wood bark. The phytochemical investigation revealed the existence of phenolics and alkaloids in methanolic crude wood bark extract and all fractions. Meanwhile, the presence of flavonoids was shown in chloroform and methanol fractions. The highest total phenolic content was demonstrated by methanol fraction with 254.34 ± 16.86 mg GAE/g extract. Meanwhile, the highest total flavonoid content was interestingly displayed in chloroform fraction with 15.33 ± 0.26 mg RE/g extract. The crude extract and methanol fraction had a profound antioxidant activity with IC50 values of less than 15.63 ppm and 8.83 ppm, respectively. A strong correlation was shown between the antioxidant activity and total phenolic contents through correlation analysis. Thus, the D. suffruticosa wood bark could be considered a potential natural source of antioxidants.


Keywords


Dillenia suffruticosa wood bark; antioxidant activity; total phenolics, total flavonoids; antioxidant and phenolic content correlation

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References


Abubakar, S., Al-Mansoub, M. A., Murugaiyah, V., & Chan, K. L. (2019). The phytochemical and anti-inflammatory studies of Dillenia suffruticosa leaves. Phytotherapy Research, 33(3), 660–675. https://doi.org/10.1002/ptr.6255

Ahmad, F. B., & Holdsworth, D. K. (1995). Traditional medicinal plants of sabah, Malaysia part III. The rungus people of kudat. Pharmaceutical Biology, 33(3), 262–264. https://doi.org/10.3109/13880209509065377

Armania, N., Saiful, L., Noorhidayah, S., Safinar, I., Biau, J., Wei, K., Noreen, H., & Hamid, A. (2013). Dillenia suffruticosa exhibited antioxidant and cytotoxic activity through induction of apoptosis and G 2 / M cell cycle arrest. Journal of Ethnopharmacology, 146(2), 525–535. https://doi.org/10.1016/j.jep.2013.01.017

Bendary, E., Francis, R. R., Ali, H. M. G., Sarwat, M. I., & El Hady, S. (2013). Antioxidant and structure–activity relationships (SARs) of some phenolic and anilines compounds. Annals of Agricultural Sciences, 58(2), 173–181. https://doi.org/10.1016/j.aoas.2013.07.002

Chen, G. L., Fan, M. X., Wu, J. L., Li, N., & Guo, M. Q. (2019). Antioxidant and anti-inflammatory properties of flavonoids from lotus plumule. Food Chemistry, 277, 706–712. https://doi.org/10.1016/j.foodchem.2018.11.040

Cies̈la, Ł. M., Waksmundzka-Hajnos, M., Wojtunik, K. A., & Hajnos, M. (2015). Thin-layer chromatography coupled with biological detection to screen natural mixtures for potential drug leads. Phytochemistry Letters, 11, 445–454. https://doi.org/10.1016/j.phytol.2015.02.005

Dawood Shah, M., Seelan Sathiya Seelan, J., & Iqbal, M. (2020). Phytochemical investigation and antioxidant activities of methanol extract, methanol fractions and essential oil of Dillenia suffruticosa leaves. Arabian Journal of Chemistry, 13(9), 7170–7182. https://doi.org/10.1016/j.arabjc.2020.07.022

Gacche, R. N., & Jadhav, S. G. (2012). Antioxidant activities and cytotoxicity of selected coumarin derivatives: preliminary results of a structure-activity relationship study using computational tools. Journal of Experimental and Clinical Medicine, 4(3), 165–169. https://doi.org/10.1016/j.jecm.2012.04.007

Grigalius, I., & Petrikaite, V. (2017). Relationship between antioxidant and anticancer activity of trihydroxyflavones. Molecules, 22(12). https://doi.org/10.3390/molecules22122169

Gunadi, D., Oramahi, H. ., & Tavita, G. E. (2017). Studi tumbuhan obat pada etnis dayak di desa gerantung Kecamatan Monterado Kabupaten Bengkayang. Jurnal Hutan Lestari, 5(2), 425–436. http://eprints.umm.ac.id/44014/%0Ahttp://jurnal.untan.ac.id/index.php/jmfkh/article/view/20089/16490

Kainama, H., Fatmawati, S., Santoso, M., Papilaya, P. M., & Ersam, T. (2020). The relationship of free radical scavenging and total phenolic and flavonoid contents of Garcinia lasoar PAM. Pharmaceutical Chemistry Journal, 53(12), 1151–1157. https://doi.org/10.1007/s11094-020-02139-5

Kessler, A., & Kalske, A. (2018). Plant secondary metabolite diversity and species interactions. Annual Review of Ecology, Evolution, and Systematics, 49, 115–138. https://doi.org/10.1146/annurev-ecolsys-110617-062406

Kristiningrum, N., Amaliyah, E. A., & Pratoko, D. K. (2020). Phytochemical screening, antioxidant and antibacterial activities of ethanol extract and fractions of Aleurites moluccana (L.) willd. leaves. Tropical Journal of Natural Product Research, 4(11), 895–898. https://doi.org/10.26538/tjnpr/v4i11.9

Marliyana, S. D., Mujahidin, D., & Syah, Y. M. (2019). Transformation reaction of prenylated chalcone of pinostrobin derivative and their antibacterial activity. IOP Conference Series: Materials Science and Engineering, 509(1), 22–27. https://doi.org/10.1088/1757-899X/509/1/012133

Abubakar, S., Al-Mansoub, M. A., Murugaiyah, V., & Chan, K. L. (2019). The phytochemical and anti-inflammatory studies of Dillenia suffruticosa leaves. Phytotherapy Research, 33(3), 660–675. https://doi.org/10.1002/ptr.6255

Ahmad, F. B., & Holdsworth, D. K. (1995). Traditional medicinal plants of sabah, Malaysia part III. The rungus people of kudat. Pharmaceutical Biology, 33(3), 262–264. https://doi.org/10.3109/13880209509065377

Armania, N., Saiful, L., Noorhidayah, S., Safinar, I., Biau, J., Wei, K., Noreen, H., & Hamid, A. (2013). Dillenia suffruticosa exhibited antioxidant and cytotoxic activity through induction of apoptosis and G 2 / M cell cycle arrest. Journal of Ethnopharmacology, 146(2), 525–535. https://doi.org/10.1016/j.jep.2013.01.017

Bendary, E., Francis, R. R., Ali, H. M. G., Sarwat, M. I., & El Hady, S. (2013). Antioxidant and structure–activity relationships (SARs) of some phenolic and anilines compounds. Annals of Agricultural Sciences, 58(2), 173–181. https://doi.org/10.1016/j.aoas.2013.07.002

Chen, G. L., Fan, M. X., Wu, J. L., Li, N., & Guo, M. Q. (2019). Antioxidant and anti-inflammatory properties of flavonoids from lotus plumule. Food Chemistry, 277, 706–712. https://doi.org/10.1016/j.foodchem.2018.11.040

Cies̈la, Ł. M., Waksmundzka-Hajnos, M., Wojtunik, K. A., & Hajnos, M. (2015). Thin-layer chromatography coupled with biological detection to screen natural mixtures for potential drug leads. Phytochemistry Letters, 11, 445–454. https://doi.org/10.1016/j.phytol.2015.02.005

Dawood Shah, M., Seelan Sathiya Seelan, J., & Iqbal, M. (2020). Phytochemical investigation and antioxidant activities of methanol extract, methanol fractions and essential oil of Dillenia suffruticosa leaves. Arabian Journal of Chemistry, 13(9), 7170–7182. https://doi.org/10.1016/j.arabjc.2020.07.022

Gacche, R. N., & Jadhav, S. G. (2012). Antioxidant activities and cytotoxicity of selected coumarin derivatives: preliminary results of a structure-activity relationship study using computational tools. Journal of Experimental and Clinical Medicine, 4(3), 165–169. https://doi.org/10.1016/j.jecm.2012.04.007

Grigalius, I., & Petrikaite, V. (2017). Relationship between antioxidant and anticancer activity of trihydroxyflavones. Molecules, 22(12). https://doi.org/10.3390/molecules22122169

Gunadi, D., Oramahi, H. ., & Tavita, G. E. (2017). Studi tumbuhan obat pada etnis dayak di desa gerantung Kecamatan Monterado Kabupaten Bengkayang. Jurnal Hutan Lestari, 5(2), 425–436. http://eprints.umm.ac.id/44014/%0Ahttp://jurnal.untan.ac.id/index.php/jmfkh/article/view/20089/16490

Kainama, H., Fatmawati, S., Santoso, M., Papilaya, P. M., & Ersam, T. (2020). The relationship of free radical scavenging and total phenolic and flavonoid contents of Garcinia lasoar PAM. Pharmaceutical Chemistry Journal, 53(12), 1151–1157. https://doi.org/10.1007/s11094-020-02139-5

Kessler, A., & Kalske, A. (2018). Plant secondary metabolite diversity and species interactions. Annual Review of Ecology, Evolution, and Systematics, 49, 115–138. https://doi.org/10.1146/annurev-ecolsys-110617-062406

Kristiningrum, N., Amaliyah, E. A., & Pratoko, D. K. (2020). Phytochemical screening, antioxidant and antibacterial activities of ethanol extract and fractions of Aleurites moluccana (L.) willd. leaves. Tropical Journal of Natural Product Research, 4(11), 895–898. https://doi.org/10.26538/tjnpr/v4i11.9

Marliyana, S. D., Mujahidin, D., & Syah, Y. M. (2019). Transformation reaction of prenylated chalcone of pinostrobin derivative and their antibacterial activity. IOP Conference Series: Materials Science and Engineering, 509(1), 22–27. https://doi.org/10.1088/1757-899X/509/1/012133

Salazar-aranda, R., P, L. A., Joel, L., & Alan, B. A. (2011). Antimicrobial and antioxidant activities of plants from Northeast of Mexico. Evidence-Based Complementary and Alternative Medicine, 2011, 536139. https://doi.org/10.1093/ecam/nep127

Sticher, O. (2008). Natural product isolation †. Natural Product Reports, 25(3), 517–554. https://doi.org/10.1039/b700306b

Tor, Y. S., Yazan, L. S., Foo, J. B., Armania, N., Cheah, Y. K., Abdullah, R., Imam, M. U., Ismail, N., & Ismail, M. (2014). Induction of apoptosis through oxidative stress-related pathways in MCF-7 , human breast cancer cells, by ethyl acetate extract of Dillenia suffruticosa. BMC Complementary and Alternative Medicine, 14, 55. https://doi.org/https://doi.org/10.1186/1472-6882-14-55

Wiart, C., Mogana, S., Khalifah, S., Mahan, M., Ismail, S., Buckle, M., Narayana, A. K., & Sulaiman, M. (2004). Antimicrobial screening of plants used for traditional medicine in the state of Perak , Peninsular Malaysia. Fitoterapia, 75, 68–73. https://doi.org/10.1016/j.fitote.2003.07.013

Yazan, L. S., & Armania, N. (2014). Dillenia species : A review of the traditional uses , active constituents and pharmacological properties from pre-clinical studies. Pharmaceutical Biology, 52(7), 890–897. https://doi.org/10.3109/13880209.2013.872672




DOI: http://dx.doi.org/10.12928/pharmaciana.v11i2.20674

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Pharmaciana
ISSN Print: 2088-4559 | ISSN Online: 2477-0256
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