The relationship between NFκβ-p65 and PGE2 gene expression in osteoarthritis synoviocyte cells after mesenchymal stem cell Wharton Jelly Treatment

Vivi Sofia

Abstract


The genes involved in cartilage degradation in the pathogenesis of osteoarthritis ( OA) are the Nuclear Factor Kappa Beta (NFĸβ) gene, which plays a vital role in modulating cartilage damage involving activation of pro-inflammatory cytokines, including prostaglandin E2 (PGE2). This pure experimental research aimed at analyzing the relationship between the expression of NFĸβ genes after administration of mesenchymal stem cells in osteoarthritis synoviocytes in vitro from Wharton's Jelly (WJ-MSCs). The samples were isolated from synovial tissues of patients with osteoarthritis who underwent Total Knee Replacement (TKR) surgery and then divided into six treatment groups, each with four replicates. Groups I and II were the synoviocyte-control classes of 24h and 48h (synoviocytes, incubated for 24 and 48 hours respectively). Groups III and IV were control groups of 24h and 48h WJ-MSCs (mesenchymal stem cells derived from the Wharton's Jelly, incubated for 24 and 48 hours respectively). Finally, Groups V and VI were the 24-hour and 48-hour co-cultural treatment groups (synoviocyte co-culture and WJ-MSCs, Incubated, for 24 and 48 hours respectively). Using qPCR, PGE2 gene and NF NFκβ gene expressions were observed in each population. Tests showed 0.61 and 0.61, respectively, after the administration of WJ-MSCs, the expressions of the NFκβ-p65 and PGE2 genes in the 24h classes. In the 48h group, meanwhile, both numbers fell to 0.15 and 0.47, respectively. These indicate a strong correlation between the NFκβ-p65 gene and PGE2 gene expressions in OA synovial cells after the administration of MSC-WJ (r = 0.903).

Keywords


NFĸβ gene; PGE2 gene; osteoarthritis; correlation

Full Text:

PDF

References


A.Krum, S., Chang, J., Miranda-Carboni, G., & Cun-Yu Wang. (2010). Novel functions for NF κ B : inhibition of bone formation. Nat Rev Rheumatol, 6(10), 607–611. https://doi.org/10.1038/nrrheum.2010.133.Novel.

Biology, C., Rigoglou, S., & Papavassiliou, A. G. (2013). The International Journal of Biochemistry The NF- ⬠B signalling pathway in osteoarthritis. International Journal of Biochemistry and Cell Biology, 45(11), 2580–2584. https://doi.org/10.1016/j.biocel.2013.08.018.

Chung, Y. C., Park, S.-M., Kim, Ji. H., Lee, G. S., Lee, J. N., & Hyun, C.-G. (2018). Anti-inflammatory effect of pratol in LPS-simulated RAW 264.7 cell via NF-kB signaling pathways. Natural Product Communications, 13(5), 547–550. https://doi.org/10.1177/1934578X1801300509.

Guillot, X., Tordi, N., Laheurte, C., Pazart, L., Prati, C., Saas, P., & Wendling, D. (2019). Local ice cryotherapy decreases synovial interleukin 6, interleukin 1β, vascular endothelial growth factor, prostaglandin-E2, and nuclear factor kappa B p65 in human knee arthritis: A controlled study. Arthritis Research and Therapy, 21(1), 1–11. https://doi.org/10.1186/s13075-019-1965-0.

Halim, D., Murti, H., Sandra, F., & Arief Boediono. (2010). Steam Cell: Dasar Teori dan Aplikasi Klinis. Erlangga.

Ibrahim, A. M., Elgharabawi, N. M., Makhlouf, M. M., & Ibrahim, O. Y. (2015). Chondrogenic Differentiation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells In Vitro. 675(June), 667–675. https://doi.org/10.1002/jemt.22520.

Kim, J., & Yoo, J. J. (2018). Therapeutics in Osteoarthritis Based on an Understanding of Its Molecular Pathogenesis. https://doi.org/10.3390/ijms19030674.

Lawson, M. A., Parrott, J. M., Mccusker, R. H., Dantzer, R., Kelley, K. W., & Connor, J. C. O. (2013). Intracerebroventricular administration of lipopolysaccharide induces indoleamine-2 , 3-. Journal of Neuroinflammation, 10(1), 1–9. https://doi.org/10.1186/1742-2094-10-87.

Luan, H., Zhang, Q., Wang, L., Wang, C., Zhang, M., Xu, X., Zhou, H., Li, X., Xu, Q., He, F., Yuan, J., & Lv, Y. (2014). OM85-BV induced the productions of IL-1β, IL-6, and TNF-α via TLR4- and TLR2-Mediated ERK1/2/NFkβPathway in RAW264.7 Cells. Journal of Interferon and Cytokine Research, 34(7), 526–536. https://doi.org/10.1089/jir.2013.0077.

Megias, J., Guillen, M. I., Clerigues, V., Rojo, A. I., Antonio Cuadrado, Castejon, M. A., Gomar, F., & Alcaraz, M. J. (2009). Heme oxygenase-1 induction modulates microsomal prostaglandin E synthase-1 expression and prostaglandin E 2 production in osteoarthritic chondrocytes. Biochemical Pharmacology, 77, 1806–1813. https://doi.org/10.1016/j.bcp.2009.03.009.

Prosperi, M. C. F., Fabbiani, M., Fanti, I., Zaccarelli, M., Colafigli, M., Mondi, A., Avino, A. D., Borghetti, A., Cauda, R., & Giambenedetto, S. Di. (2012). Predictors of first-line antiretroviral therapy discontinuation due to drug-related adverse events in HIV-infected patients : a retrospective cohort study. BMC Infectious Disease.

Rasheed, N., Alghasham, A., & Rasheed, Z. (2016). Lactoferrin from Camelus dromedarius Inhibits Nuclear Transcription Factor ‑ kappa B Activation , Cyclooxygenase ‑ 2 Expression and Prostaglandin E2 Production in Stimulated Human Chondrocytes. 135–141. https://doi.org/10.4103/0974-8490.175612.

Rosengren, S., Boyle, D. L., & Firestein, G. S. (2007). Acquisition, Culture, and Phenotyping of Synovial Fibroblasts. 135, 365–375.

Saito, T. (2017). Molecular mechanisms underlying osteoarthritis development : Notch and NF- κ B. 1–7. https://doi.org/10.1186/s13075-017-1296-y

Signaling, N. B., & Kopitar-jerala, N. (2015). Innate Immune Response in Brain ,. 8(December), 1–9. https://doi.org/10.3389/fnmol.2015.00073.

Sun, J., Ramnath, R. D., Tamizhselvi, R., & Bhatia, M. (2009). Role of protein kinase C and phosphoinositide 3â€kinaseâ€Akt in substance Pâ€induced proinflammatory pathways in mouse macrophages. The FASEB Journal, 23(4), 997–1010. https://doi.org/10.1096/fj.08-121756.

Tornatore, L., Thotakura, A. K., Bennett, J., & Moretti, M. (2012). The nuclear factor kappa B signaling pathway : integrating metabolism with inflammation. Trends in Cell Biology, 22(11), 557–566. https://doi.org/10.1016/j.tcb.2012.08.001.

Watanabe, H., Ishii, H., Takahashi, K., & Takai, S. (2017). Suitable reference gene selection for gene expression studies in knee osteoarthritis synovium using quantitative PCR analysis. Connective Tissue Research, 00(00), 1–13. https://doi.org/10.1080/03008207.2017.1391234.

Yamada, T., Oshima, T., Yoshihara, K., Tamura, S., Kanazawa, A., Inagaki, D., Yamamoto, N., Sato, T., Fujii, S., Numata, K., Kunisaki, C., Shiozawa, M., Morinaga, S., Akaike, M., Rino, Y., Tanaka, K., Masuda, M., & Imada, T. (2010). Overexpression of MMP-13 gene in colorectal cancer with liver metastasis. Anticancer Research, 30(7), 2693–2699.

Zhang, X., Yan, K., Deng, L., Liang, J., Liang, H., Feng, D., & Ling, B. (2019). Cyclooxygenase 2 Promotes Proliferation and Invasion in Ovarian Cancer Cells via the PGE2 / NF- k B Pathway. 28, 1–13. https://doi.org/10.1177/0963689719890597.




DOI: http://dx.doi.org/10.12928/pharmaciana.v10i2.15926

Refbacks

  • There are currently no refbacks.


Copyright (c) 2021 Vivi Sofia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.


 
Pharmaciana
ISSN Print: 2088-4559 | ISSN Online: 2477-0256
Website: http://journal.uad.ac.id/index.php/PHARMACIANA
Office: Faculty of  Pharmacy, Universitas Ahmad Dahlan
Jl. Prof. Dr. Soepomo, S.H., Janturan, Warungboto, Umbulharjo, Yogyakarta, Indonesia
Kode pos 55164
Email: pharmaciana@pharm.uad.ac.id