Cox-2 inhibition activities of creams containing anguilla bicolor and sea cucumbers extract on croton oil induced inflammation in mice

Heru Sasongko, Waliyyin Razan Qanit, Raka Sukmabayu W.S, Agung Putu S.P.Kristyawan, Nining Sugihartini, Wisnu Kundarto, Dian Eka Ermawati


The fatty acids, like EPA and DHA, were known as anti-inflammation. It works on inflammatory tissue, brought by edema plasma, and changed into resolvins, protectins, and maresins by the enzyme reaction. Anguilla bicolor and Sea cucumber fish are known to contain EPA and DHA. This study analyzes the inhibition Cox-2 effect of the combination of Anguilla bicolor oil with Sea cucumber extract as an active ingredient in a cream preparation. This experiment used 7 groups of male BALB/C strain mice: normal control; negative control; positive control; A. bicolor oil cream; H. leucospilota extract cream; a combination of A. bicolor : H. leucospilota (2:1) cream; a combination of A. bicolor : H. leucospilota (1:2) cream. The anti-inflammatory activity was evaluated by the amount of inflammatory cell, the thickness epidermis, and the amount of cell expression COX-2  on mice's back skin tissue induced by croton oil (0,1 %). After 3 days, histopathological skin tissues were made. The data were analyzed statistically by One Way ANOVA followed by LSD to know each group's differences at a significance level of 0.05. The experiment results showed that the formula with the best anti-inflammatory activities is the combination of A. bicolor and H. leucospilota (2:1) cream.  The decrease of the amount of inflammatory cell (75.97%), the thickness of the epidermis (43.88%), and the amount of COX-2 cell expressions (60.52%) from the formula did not differ significantly with the positive control (p>0,05). It can be concluded that A. bicolor and H. leucospilota have anti-inflammation activity based on the experiment results.


Anti-inflammatory; Anguilla bicolor; Cox-2, Sea cucumber.

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Ahn, J. C., Chong, W.-S., Na, J. H., Yun, H. B., Shin, K. J., Lee, K. W., & Park, J. T. (2015). An Evaluation of Major Nutrients of Four Farmed Freshwater Eel Species (Anguilla japonica, A. rostrata, A. bicolor pacifica and A. marmorata). Korean Journal of Fisheries and Aquatic Sciences, 48(1), 44–50.

Calder, P. C. (2013). Omega-3 polyunsaturated fatty acids and inflammatory processes: Nutrition or pharmacology? British Journal of Clinical Pharmacology, 75(3), 645–662.

Garavito, R. M., & Dewitt, D. L. (1999). The cyclooxygenase isoforms: Structural insights into the conversion of arachidonic acid to prostaglandins. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1441(2–3), 278–287.

Haq, F. M., Santoso, H., & Syauqi, A. (2018). Analisa Kadar Protein Albumin Ikan Sidat ( Anguilla bicolor ) Air Tawar Segar dan Dikukus di Maduran Lamomgan Jurnal Sains Alami (Known Nature). 1.1(September), 13–19.

Janakiram, N. B., Mohammed, A., & Rao, C. V. (2015). Sea cucumbers metabolites as potent anti-cancer agents. Marine Drugs, 13(5), 2909–2923.

Kitajima, M., Kimura, A., & Suzuki, H. (2018). Cutting Edge: Nqo1 Regulates Irritant Contact Hypersensitivity against Croton Oil through Maintenance of Dendritic Epidermal T Cells. The Journal of Immunology, ji1701389.

Kusharto, C. M., Emi Widyasari, R. A. H., Budywiryawan, B., Sri Wiyono, E., & Sugengherisuseno, S. (2014). Nutritive Value and Fatty Acids Profile of Fresh Indonesian Eel (Anguilla bicolor) and Kabayaki. Jurnal Sains Kesihatan Malaysia, 12(1), 41–46.

Lan, M., Wan, P., Wang, Z. Y., & Huang, X. L. (2012). [GC-MS analysis of chemical components in seeds oil from Croton tiglium]. Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials.

Lane, D. J. W., & Limbong, D. (2015). Catastrophic depletion of reef-associated sea cucumbers: Resource management/reef resilience issues for an Indonesian marine park and the wider Indo-Pacific. Aquatic Conservation: Marine and Freshwater Ecosystems, 25(4), 505–517.

MacKay, D., & Miller, A. L. (2003). Nutritional Support for Wound Healing. In Alternative Medicine Review.

Moore, B., & Simmons, D. (2012). COX-2 Inhibition, Apoptosis, and Chemoprevention by Nonsteroidal Anti-inflammatory Drugs. Current Medicinal Chemistry, 7(11), 1131–1144.

Mullen, A., Loscher, C. E., & Roche, H. M. (2010). Anti-inflammatory effects of EPA and DHA are dependent upon time and dose-response elements associated with LPS stimulation in THP-1-derived macrophages. Journal of Nutritional Biochemistry, 21(5), 444–450.

Purwati, P. (2018). Reproductive Patterns of Holothuria Scabra (Echinodermata: Holothuroidea) in Indonesian Waters. Marine Research in Indonesia, 30, 47–55.

Putri, A. A. B., Yuliet, Y., & Jamaluddin, J. (2016). Analisis Kadar Albumin Ikan Sidat (Anguilla marmorata dan Anguilla bicolor) Dan Uji Aktivitas Penyembuhan Luka Terbuka Pada Kelinci (Oryctolagus cuniculus). Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 2(2), 90–95.

Sasongko, H., Efendi, N. R., Budihardjo, A., Farida, Y., Amartiwi, T., Rahmawati, A. A., Wicaksono, A., & Sugiyarto. (2017). Solvent and extraction methods effects on the quality of eel (Anguilla bicolor) oil. Journal of Physics: Conference Series, 795(1).

Sasongko, H., Saputro, B. A., Hidayati, R. W. N., Raka, S. W. S., & Sekarjati, T. A. (2018). The effect of hydrocarbon ointment containing eel fish (Anguilla bicolor bicolor) and snakehead fish (Channa striata) oil for wound healing in Wistar rats. AIP Conference Proceedings, 2019.

Sasongko, Heru, Rahmawati, A. A., Kusumastuti, N. I., & Alifa, G. R. (2019). in-Vivo Analgesic and Anti-Inflammatory Effects of Eel (Anguilla Bicolor Bicolor) Oil. Pharmaciana, 9(1), 59–70.

Serhan, C. N. (2014). Pro-resolving lipid mediators are leads for resolution physiology. Nature, 510(7503), 92–101.

Shah, B. N., Seth, A. K., & Maheshwari, K. M. (2011). A review on medicinal plants as a source of anti-inflammatory agents. Research Journal of Medicinal Plant.

Sugihartini, N., Saridewi, R., Ramdhani, U. M., Rahmawanti, F., Yuliani, S., & Sophia, V. (2017). Anti-inflammatory Activity of Camellia sinensis, l. Extract Cream Combined with Vitamin C as Antioxidant on Croton Oil-induced Inflamation in Male Mice Strain BALB/C Daya Anti-Inflamasi Krim Kombinasi Ekstrak Teh Hijau dan Vitamin C sebagai Antioksidan pa. Traditional Medicine Journal, 22(2), 2017.

Vors, C., Allaire, J., Marin, J., Lépine, M. C., Charest, A., Tchernof, A., Couture, P., & Lamarche, B. (2017). Inflammatory gene expression in whole blood cells after EPA vs. DHA supplementation: Results from the ComparED study. Atherosclerosis, 257, 116–122.

Wen, J., Hu, C., & Fan, S. (2010). Chemical composition and nutritional quality of sea cucumbers. Journal of the Science of Food and Agriculture, 90(14), 2469–2474.

Whitehouse, M. W., & Fairlie, D. P. (1994). Anti-Inflammatory activity of a holothurian (sea cucumber) food supplement in rats. Inflammopharmacology, 2(4), 411–417.

Wiranto, E., Wibowo, M. A., & Ardiningsih, P. (2016). Aktivitas Antiinflamasi Secara In-Vitro Ekstrak Teripang Butoh Keling (Holothuria leucospilota Brandt) Dari Pulau Lemukutan. Jkk, 5(1), 52–57.

Yahyav. (2012). Fatty acid composition in two sea cucumber species, Holothuria scabra and Holothuria leucospilata from Qeshm Island (Persian Gulf). African Journal of Biotechnology, 11(12), 2862–2868.


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