Antioxidant and alpha-amylase inhibitory study of Sansevieria trifasciata Prain. leaves extract

Vivi Anggia, Lusi Putri Dwita, Istikomah Istikomah


Diabetes is one of the chronic diseases whose prevalence is increasing all over the world, including in Indonesia. Diabetes mellitus is characterized by high blood glucose levels that can be overcome by, for instance, inhibiting the alpha-amylase enzymes that play a role in carbohydrate hydrolysis. Several studies have proved that active antioxidant substances can also support the treatment of diabetic patients. Snake plant (Sansevieria trifasciata Prain.), or locally known as lidah mertua in Indonesia, is a traditional herbal used to treat diabetes. This study investigated the activity of the ethanol extract of S. trifasciata leaves in inhibiting α-amylase. The leaves were extracted by maceration with ethanol 70%, followed by phytochemical screening. The antioxidant activity was determined using the DPPH method, while the inhibition of α-amylase was measured using UV-Vis spectrophotometer at 524nm wavelength. The results showed that IC50 of the antioxidant activity was 1527.55 ppm, five times greater than the positive control, i.e., vitamin C. In the case of α-amylase enzyme, the IC50 of the ethanol extract of S. trifasciata leaves was 158.31 μg/ml and 0.26 times more potential than acarbose. The study has proved that the crude extract of S. trifasciata leaves has the potential as one of the antidiabetics and antioxidants that can be studied further.



Sansevieria trifasciata Prain., α-amylase, antidiabetic

Full Text:



Findik, E., Ceylan, M., Elmastas, M., 2011. Isoeugenol-based novel potent antioxidants: Synthesis and reactivity. European Journal of Medicinal Chemistry, 46 (9):4618-4624.

Fitrianingsih, S.P., Indra, T.M., Ratu, C., Desirian, D., Ratih, A., 2016. Uji aktivitas penghambatan alfa amilase ekstrak daun Tithonia diversifolia secara in vitro. Prosiding SNaPP, 6 (1).

Harborne, J.B., 1987. Phytochemical methods. In: applied phytochemistry method. Kosasih, P. and I. Soediro (Eds.). Penerbit ITB, Bandung.

Lee, S.H., Mi-Hwa, P, Soo-Jin, H., Sung-Myung, K., Seok-Chun, K., Ji-Sook, H., You-Jin J., 2010. Dieckol isolated from Ecklonia cava inhibits α-glucosidase and α-amylase in vitro and alleviates postprandial hyperglycemia in streptozotocin-induced diabetic mice, Food and Chemical Toxicology, 48 (10): 2633–2637.

Miura, T., Nosaka, K., Ishii, H., and Ishida, T., 2005. Antidiabetic effect of nitobegiku, the herb Tithonia diversifolia, in KK-Ay diabetic mice, Biol. Pharm. Bull., 28 (11): 2152-2154.

PERKENI (Perkumpulan Endokrinologi Indonesia), 2015. Pengelolaan dan Pencegahan Diabetes Melitus Tipe 2 di Indonesia 2015. PB PERKENI.

Laimeheriwa, C., Adeanne, C.W., Widya, A.L. 2014. Uji efek ekstrak etanol daun lidah mertua (Sansevieria trifasciata Prain) terhadap penurunan kadar gula darah tikus putih jantan galur wistar (Rattus norvegicus L.). PHARMACON Jurnal Ilmiah Farmasi, 3 (3): 255–262.

Mehta J.L., Rasouli N., Sinha A.K., Molavi B., 2006, Oxidative stress in diabetes: a mechanistic overview of its effects on atherogenesis and myocardial dysfunction. Int J Biochem Cell Biol, 38:794–803.

Molyneux, P., 2004. The use of stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity, Songklanakarin J. Sci. Technol, 26(2). 211-219.

Nasri, H., Rafieian-Kopaei M., 2013. Tubular kidney protection by antioxidants, Iran. J. Public. Health, 42: 1194-6.

Pattanayak, S.P., Mazumder, P.M., Sunitha, P., 2011. Total phenolic content, flavonoid content and in vitro antioxidant activities of Dentrophthoe falcate (L.F.) Ettingsh. Int. J. PharmTech Res., 3:1392-1406.

Qomariyah, N., 2012. Antidiabetic effects of a decoction of leaves of Sansevieria trifasciata in alloxan-induced diabetic white rats (Rattus norvegicus L.). ITB J. Sci., 44A (4): 308-316.

Rwawiire. S., Blanka, T., 2015. Morphological, thermal, and mechanical characterization of Sansevieria trifasciata fibers. Journal of Natural Fibers, 12 (3): 201-210.

Scherer, R., Godoy, H. T., 2009. Antioxidant activity index (AAI) by the 2,2-diphenyl-1-picrylhydrazyl method, Food Chemistry., 112 (3): 654-658.

Sunarni, T., Pramono, S., Asmah, R., 2007. Flavonoid antioksi dan penangkap radikal dari daun kepel (Stelechocarpus burahol (Bl.) Hook f.& Th.). Majalah Farmasi Indonesia, 18 (3): 111-116.

Talukdar, N., Dutta, A.M., Raja, C., Karabi, D., 2017. Screening of phytochemicals, antioxidant and inhibitory effect on alpha-amylase by ethanolic extract of Elaeocarpus Ganitrus (Bark). IJPSR,8(12):5270-5275.

Thalapaneni, N.R., Chidambran, K.A., Ellappan, T., Sabapathi, M.L., Mandal, S.C., 2008. Inhibition of carbohydrate digestive enzymes by Talinum portulacifolium (Forssk) leaf extract. Journal of Complementary and Integrative Medicine, 5 (1).

Wickramaratne, M.N., Punchihewa, J.C., Wickramaratne, D.B.M., 2016. In-vitro alpha-amylase inhibitory activity of the leaf extracts of Adenanthera pavonina, BMC Complement Altern Med., 16 (1): 466.


Article Metrics

Abstract view : 191 times
PDF - 89 times


  • There are currently no refbacks.

Copyright (c) 2019 Pharmaciana

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.


Pharmaciana indexed by:

Google ScholarDirectory of Open Access JournalIndonesian Scientific Journal Database (ISJD)Indonesian Publication Index (IPI)CrossrefBielefeld Academic Search Engine (BASE)Sherpa RomeoOpen Academic Journal Index (OAJI)





Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.