Identifying active compounds of soursop ethanolic fraction as α-glucosidase inhibitor

Eko Mugiyanto, Agung Nur Cahyanta, I Made Agus Sunadi Putra, Siswa Setyahadi, Partomuan Simanjuntak

Abstract


Postprandial hyperglycemia is triggered by two enteric proteins (α-amylase and α-glucosidase) connected to the brush border of intestinal cells. In diabetic populations, Type 2 Diabetes Mellitus is more prevalent, and α-glucosidase inhibition is the suitable therapy for delaying glucose intake after meals. This study was designed to investigate chemical compounds for their potential inhibitory effects on α-glucosidase. It employed a spectrophotometric method with p-nitrophenyl-α-D-glucopyranose from Saccharomyces cerevisiae as an alpha-glucosidase substrate. The ethanolic fraction was acquired from assay- or activity-guided fractionation. The findings showed that the ethanolic fraction of soursop leaves exhibited the most significant inhibitory activity with an IC50 of 0.17 μg/ml. The data analysis and active compounds identification were carried out by LC-MS and FT-IR.  The active compounds of the resulted mixture comprised of Muricatin C, cis-Reticulatacin-10-one, and 3-Methylquercetin 7-[galactosyl-(1->4)-glucoside].


Keywords


Muricatin, cis-Reticulin; Methylquercetin; Saccharomyces cerevisiae

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DOI: http://dx.doi.org/10.12928/pharmaciana.v9i2.10105

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