Toxicity test of Kedayan root infusion (Aristolochia sp.) using brine shrimp lethality test method
Abstract
Kedayan roots are still used by the Lundayeh Dayak people as traditional medicine. This plant is one of the Aristolochia sp. species whose production and distribution as traditional medicine or food supplements are prohibited by the Food and Drug Supervisory Agency of the Republic of Indonesia because it has side effects that are harmful to the body. The purpose of this study was to determine the toxicity of kedayan root infusion using the Brine Shrimp Lethality Test (BSLT) method based on the LC50 value. Toxicity test was carried out by varying the concentration of Kedayan root infusion, namely 100, 500, and 1000 ppm, as an intervention against Artemia salina Leach with a negative control in the form of 2.8% saline solution for 24 hours with 3 replications, then observing the presence or absence of movement of Artemia salina L. Larvae. Test results data in the form of % mortality obtained, then analyzed using the probit regression analysis method to determine the LC50 value. The results of this study indicate that the kedayan root infusion has very high toxicity, the LC50 value is 101.9278 ppm.
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Aigbe, F. R. et al. (2019) ‘Evaluation of The Toxicity Potential of Acute and Sub-acute Exposure to The Aqueous Root Extract of Aristolochia Ringens Vahl. (Aristolochiaceae)’, Journal of Ethnopharmacology, 244, pp. 1–10.
Anger, E. E., Yu, F. and Li, J. (2020) ‘Aristolochic Acid-Induced Nephrotoxicity : Molecular Mechanisms and Potential Protective Approaches’, Molecular Sciences, 21(1157), pp. 1–20.
Aras, T. R. (2013) Uji Toksisitas Ekstrak Teripang Holothuria Scabra Terhadap Artemia Salina. Skripsi. Fakutas Ilmu Kelautan Dan Perikanan, Universitas Hasanuddin, Makassar.
Arcanjo (2012) ‘Bioactivity Evaluation Against Artemia Salina Leach of Sedicinal Plants Used in Brazilian Northeastern Folk Medicine’, Brazilian Journal of Biology, 72(3), pp. 505–509.
Asaduzzaman, M. et al. (2015) ‘Cytotoxic (Brine Shrimp Lethality Bioassay) and Antioxidant Investigation of Barringtonia Acutangula (L.)’, International Journal of Pharma Sciences and Research, 6(8), pp. 1179–1185.
Bourhia, M. et al. (2019) ‘Phytochemical screening and toxicological study of Aristolochia baetica Linn roots: histopathological and biochemical evidence’, Journal of Toxicology, pp. 1–7.
Chairunnisa, S., Wartini, N. M. and Suhendra, L. (2019) ‘Pengaruh Suhu dan Waktu Maserasi Terhadap Karakteristik Ekstrak Daun Bidara (Ziziphus mauritiana L.) Sebagai Sumber Saponin’, Jurnal Rekayasa Dan Manajemen Agroindustri, 7(4), pp. 551–560.
Elsyana, V., Bintang, M. and Priosoeryanto, B. P. (2016) ‘Cytotoxicity and Antiproliferative Activity Assay of Clove Mistletoe ( Dendrophthoe pentandra ( L .) Miq .) Leaves Extracts’, Hindawi, 2016, pp. 1–5.
Hasrianti, Nururrahmah and Nurasia (2016) ‘Pemanfaatan Ekstrak Bawang Merah dan Asam Asetat Sebagai Pengawet Alami Bakso’, Jurnal Dinamika, 7(1), pp. 9–30.
Herman (2011) Pemanfaatan Akar Kedayan (Aristolochia sp) Sebagai Obat Tradisional Oleh Masyarakat Suku Dayak Merap Desa Gong Solok Kecamatan Malinau Selatan Kabupaten Malinau. Skripsi. Politeknik Pertanian Negeri Samarinda, Samarinda.
Hong, D. et al. (2020) ‘Design, Synthesis, Brine Shrimp Lethality and Cytotoxicity of Some Novel 17a-aza-D-homo-androster-17-one Derivatives’, Natural Product Research, pp. 1–7.
Irnawati, Ode, L. and Haqqa, I. (2016) ‘Uji Toksisitas Akut Infusa Kulit Batang Ubi Kayu ( Manihot esculenta Crantz ) menggunakan Metode Brine Shrimp Lethality Test ( BSLT )’, Pharmauho, 2(1), pp. 10–12.
Jelita, S. F. et al. (2020) ‘Uji Toksisitas Infusa Acalpha Siamensis Sengan Metode Brine Shrimp Lethlity Test (BSLT)’, 18(1), pp. 14–22.
Khafidhoh, Z., Dewi, S. S. and Iswara, A. (2015) ‘Efektvitas Infusa Kulit Jeruk Purut ( Citrus hystrix DC .) Terhadap Pertumbuhan Candida albicans Penyebab Sariawan Secara In Vitro’, The 2nd University Research Coloquium 2015, pp. 31–37.
Luciano, R. L. and Perazella, M. A. (2015) ‘Aristolochic Acid Nephropathy: Epidemiology, Clinical Presentation, and Treatment’, Drug Safety, pp. 55–64.
Nastiti, M. et al. (2017) ‘Skrining Fitokimia dan Uji Toksisitas Daun Terap ( Artocarpus elasticus ) Dengan Metode Brine Shrimp Lethlity Test (BSLT)’, Prosiding Seminar nasional kimia, 60, pp. 69–73.
Nault, J. C. and Letouzé, E. (2019) ‘Mutational Processes in Hepatocellular Carcinoma: The Story of Aristolochic Acid’, Seminars in Liver Disease, pp. 334–340.
Ningdyah, A. W., Alimuddin, A. H. and Jayuska, A. (2015) ‘Uji Toksisitas Dengan Metode BSLT ( Brine Shrimp Lethality Test ) Terhadap Hasil Fraksinasi Ekstrak Kulit Buah Tampoi (Baccaurea Macrocarpa)’, JKK, 4(1), pp. 75–83.
Nur Zaki Hanifah (2015) Uji Toksisitas Akut Ekstrak Metanol Daun sirsak (Annona muricata L) Terhadap Larva Artemia Salina Leach Dengan Metode Brine Shrimp Lethality Test (BSLT). Skripsi. Program Studi Pendidikan Dokter Universitas Islam Negeri Syarif Hidayatullah, Jakarta.
El Omari, N. et al. (2020) ‘Toxicological Investigations of Aristolochia longa Root Extracts’, Journal of Toxicology, pp. 1–11.
Pecoraro, R. et al. (2020) ‘Artemia Salina: A Microcrustacean to Assess Engineered Nanoparticles Toxicity’, Microscopy Research and Technique, pp. 6–11.
Prasetyowati, K. L. (2012) Analisis Senyawa Karsinogenik Nitrosodipropilamin (NDPA) Pada Sosis Mentah Dengan Headspace-Single Drop Mircoextraction-Gas Chromatography-Flame Ionization Detector (HS-SDME-GC-FID). Skripsi. Fakultas Sains dan Teknologi Universitas Airlangga, Surabaya.
Prayudo, A. N. et al. (2015) ‘Koefisien Transfer Massa Kurkumin dari Temulawak’, Jurnal Ilmiah Widya Teknik, 14(1), pp. 26–31.
Salam, an abdi (2021) Uji Toksisitas Ekstrak Metanol Daun Ficus elastica Roxb. ex Hornem dan Ficus Benjamina Terhadap Karva Artemia salina Leach Dengan Menggunakan Metode Brine Shrimp Lethality Test (BSLT). Skripsi. Universitas Islam Negeri Sunan Ampel, Surabaya.
Setiawan, A. and Muchlas, F. (2019) ‘Kajian Etnobotani Tumbuhan Obat Suku Dayak Lundayeh di Desa Kaliamok Kecamatan Malinau Utara Kabupaten Malinau Sebagai Booklet Untuk Masyarakat’, Borneo Journal Of Biology education, 1(1), pp. 51–67.
Surya, A. (2018) ‘Toksitas Ekstrak Metanol Kulit Jengkol (Pithecellobium Jiringa) Dengan Metode Brine Shrimp Lethality Test Terhadap Larva Udang (Artemia Salina)’, Jurnal Rekayasa Sistem Industri, 3(2), pp. 149–153.
Wijaya, D. R., Paramitha, M. and Putri, N. P. (2019) ‘Ekstraksi Oleoresin Jahe Gajah (Zingiber officinale var. Officinarum) Dengan Metode Sokletasi’, Jurnal Konversi, 8(1), pp. 9–16.
Zhang, H. M. et al. (2019) ‘Recognition of The Toxicity of Aristolochic Acid’, Journal of Clinical Pharmacy and Therapeutics, pp. 157–162.
Zhang, Y. et al. (2012) ‘An Improved Brine Shrimp Larvae Lethality Microwell Test Method’, Toxicology Mechanisms and Methods, 22(1), pp. 23–30.
DOI: http://dx.doi.org/10.12928/mf.v19i2.21045
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