Bioprocess of of Astaxanthin Production as Functional Food from Aurantiochytrium Microalgae: A Review
DOI:
https://doi.org/10.26555/chemica.v8i2.21954Keywords:
Astaxanthin, Aurantiochytrium, Microalgae, Functional nutritionAbstract
The Covid-19 pandemic has increased human needs for nutrition including functional food and nutraceutical products in line with the need to boost immunity to resist viral infection, maintain a healthy life, and limit fatalities. In this regard, the choice to use functional food and nutraceuticals seems to be a promising panacea. This paper aims to examine the potential of the microalgae species Aurantiochytrium, which is commonly found in the mangrove ecosystem. This microalgae species has received a lot of attention from researchers because of its high content of lipids and other high-value-added components as well as its fast growth and resistance to environmental stress. With current bioprocess technology, Aurantiochytrium microalgae can be deployed to produce high-value-added components such as astaxanthin. During the current pandemic, the need for this product is increasing as raw material for drugs, health supplements, antioxidants, and vaccine adjuvants that are in direct contact with efforts to combat the human coronavirus and prevent the spread of viral diseases. In general, producing functional nutritional products from  Aurantiochytrium microalgae requires several stages starting from the isolation and screening of microalgae, cultivation, extraction of the desired components and finally downstream processing for purification and packaging the product. Although Aurantiochytrium microalgae is found in the mangrove ecosystem, unfortunately, studies on the production potential of functional nutrients from microalgae Aurantiochytrium native strains from Indonesia have been rarely pubslihed. Therefore, it is expected that this study will become a fundamental basis for further research in this field and increasing attention for the production of functional nutrients from  Aurantiochytrium microalgae isolated from mangrove forests in Indonesia. An early insight into bioprocess technology modes such as appropriate isolation techniques, cultivation, and extraction to produce astaxanthin is provided.
References
. Aasen, I. M., Ertesvåg, H., Marita, T., et al. (2016) ‘Thraustochytrids as production organisms for docosahexaenoic acid ( DHA ), squalene , and carotenoids’, Applied Microbiology and Biotechnology, (1), pp. 4309–4321. doi: 10.1007/s00253-016-7498-4.
. Aasen, I. M., Ertesvåg, H., Heggeset, T. M. B., et al. (2016) ‘Thraustochytrids as production organisms for docosahexaenoic acid (DHA), squalene, and carotenoids’, Applied Microbiology and Biotechnology, 100(10), pp. 4309–4321. doi: 10.1007/s00253-016-7498-4.
. Augusto, V. et al. (2019) ‘Aurantiochytrium sp . meal as DHA source in Nile tilapia diet , part I : Growth performance and body composition’, 1(April 2018), pp. 390–399. doi: 10.1111/are.13887.
. Barclay B (2012) ‘Key achievements and marketing considerations in the development of an algae-based fermentation technology: DHA from Schizocytrium’, in 5th Federal Algae Roundtable Meeting, Munich, 2012.
. Béligon, V. et al. (2016) ‘Microbial lipids as potential source to food supplements’, Current Opinion in Food Science, 7, pp. 35–42. doi: 10.1016/j.cofs.2015.10.002.
. Bongiorni, L. et al. (2005) ‘Thraustochytrium gaertnerium sp. nov.: A new thraustochytrid stramenopilan protist from mangroves of Goa, India’, Protist, 156(3), pp. 303–315. doi: 10.1016/j.protis.2005.05.001.
. Borowitzka, M. A. (1992) ‘Algal biotechnology products and processes - matching science and economics’, pp. 267–279.
. Burja, A. M. et al. (2006) ‘Isolation and characterization of polyunsaturated fatty acid producing Thraustochytrium species: Screening of strains and optimization of omega-3 production’, Applied Microbiology and Biotechnology, 72(6), pp. 1161–1169. doi: 10.1007/s00253-006-0419-1.
. Byreddy, A. R. (2016) ‘Thraustochytrids as an alternative source of omega-3 fatty acids , carotenoids and enzymes’, 28(3), pp. 68–70. doi: 10.1002/lite.201600019.
. Chandrasekaran, K., Roy, R. K. and Chadha, A. (2018) ‘Docosahexaenoic acid production by a novel high yielding strain of Thraustochytrium sp. of Indian origin: Isolation and bioprocess optimization studies’, Algal Research, 32(March), pp. 93–100. doi: 10.1016/j.algal.2018.03.011.
. Chang, J. P., Pariante, C. M. and Su, K. (2020) ‘Omega-3 fatty acids in the psychological and physiological resilience against COVID-19’, Prostaglandins, Leukotrienes and Essential Fatty Acids, p. 102177. doi: 10.1016/j.plefa.2020.102177.
. Charoonnart, P., Purton, S. and Saksmerprome, V. (2018) ‘Applications of microalgal biotechnology for disease control in aquaculture’, Biology, 7(2), pp. 1–14. doi: 10.3390/biology7020024.
. Chew, K. W. et al. (2017) ‘Microalgae biorefinery: High value products perspectives’, Bioresource Technology, 229, pp. 53–62. doi: 10.1016/j.biortech.2017.01.006.
. Cuellar-Bermudez, S. P. et al. (2015) ‘Extraction and purification of high-value metabolites from microalgae: Essential lipids, astaxanthin and phycobiliproteins’, Microbial Biotechnology, 8(2), pp. 190–209. doi: 10.1111/1751-7915.12167.
. Devasia, V. L. A. and Muraleedharan, U. D. (2012) ‘Polysaccharide-degrading enzymes from the marine protists, thraustochytrids’, Biotechnol. Bioinf. Bioeng, 2(1), pp. 617–627. doi: 10.1038/srep08611.
. Evonik (2018) DSM and Evonik establish Veramaris joint venture.
. Evonik (2019) No Evonik and DSM joint venture Veramaris named world’s best-selling algae-based omega-3 supplier to aquacultureTitle.
. Fang, N. et al. (2019) ‘De novo synthesis of astaxanthin: From organisms to genes’, Trends in Food Science and Technology, 92(11), pp. 162–171. doi: 10.1016/j.tifs.2019.08.016.
. Fossier Marchan, L. et al. (2018) ‘Taxonomy, ecology and biotechnological applications of thraustochytrids: A review’, Biotechnology Advances, 36(1), pp. 26–46. doi: 10.1016/j.biotechadv.2017.09.003.
. Galanakis, C. M. et al. (2021) ‘Trends in Food Science & Technology Innovations and technology disruptions in the food sector within the COVID-19 pandemic and post-lockdown era’, Trends in Food Science & Technology, 110(July 2020), pp. 193–200. doi: 10.1016/j.tifs.2021.02.002.
. Gao, M. et al. (2013) ‘Isolation and characterization of Aurantiochytrium species: High docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp. SD116’, Journal of Oleo Science, 62(3), pp. 143–151. doi: 10.5650/jos.62.143.
. Goa, P. (1986) ‘Thraustochytrid Fungi Associated with Marine Algae’, 15(June), pp. 121–122.
. Gohil, N. et al. (2019) ‘Engineering Strategies in Microorganisms for the Enhanced Production of Squalene : Advances , Challenges and Opportunities’, 7(March), pp. 1–24. doi: 10.3389/fbioe.2019.00050.
. Guerin, M., Huntley, M. E. and Olaizola, M. (2003) ‘Haematococcus astaxanthin: Applications for human health and nutrition’, Trends in Biotechnology, 21(5), pp. 210–216. doi: 10.1016/S0167-7799(03)00078-7.
. Han, B. and Hoang, B. X. (2020) ‘Journal of Infection and Public Health Opinions on the current pandemic of COVID-19 : Use functional food to boost our immune functions’, Journal of Infection and Public Health, 13(12), pp. 1811–1817. doi: 10.1016/j.jiph.2020.08.014.
. Hoang, M. H. et al. (2014) ‘Extraction of squalene as value-added product from the residual biomass of Schizochytrium mangrovei PQ6 during biodiesel producing process’, Journal of Bioscience and Bioengineering, 118(6), pp. 632–639. doi: 10.1016/j.jbiosc.2014.05.015.
. Honda, D. et al. (1998) ‘Schizochytrium limacinum sp. nov., a new thraustochytrid from a mangrove area in the west Pacific Ocean’, Mycological Research, 102(4), pp. 439–448. doi: 10.1017/S0953756297005170.
. Hutari, A. et al. (2016) ‘Simposium BioProScale ke-4, Berlin.’, in 4th Simposium BioProScale, Berlin., Berlin. Berlin, Germany: Technische Universität Berlin. Germany.
. Hutari, A. and Neuebauer, P. (2016) ‘2’, in 8th Indonesian Seminar of Indonesian Society for Microbiology. Jakarta, Indonesia: Indonesian Seminar of Indonesian Society for Microbiology.
. Ip, P. F. and Chen, F. (2005) ‘Production of astaxanthin by the green microalga Chlorella zofingiensis in the dark’, Process Biochemistry, 40(2), pp. 733–738. doi: 10.1016/j.procbio.2004.01.039.
. Irene, S., Kalmpourtzidou, Ð. and Cena, H. (2021) ‘The potential role of nutrition in mitigating the psychological impact of COVID-19 in healthcare workers’, 22(December 2020), pp. 6–8. doi: 10.1016/j.nfs.2020.12.002.
. Janthanomsuk, P., Verduyn, C. and Chauvatcharin, S. (2015) ‘Improved docosahexaenoic acid production in Aurantiochytrium by glucose limited pH-auxostat fed-batch cultivation’, Bioresource Technology, 196, pp. 592–599. doi: 10.1016/j.biortech.2015.08.023.
. Jia, Q. et al. (no date) ‘A Bioreactor System Based on a Novel Oxygen Transfer Method’.
. Jones, E. B. G. (2000) ‘Marine fungi : some factors influencing biodiversity’, pp. 53–73.
. Kaya, K. et al. (2011) ‘Thraustochytrid Aurantiochytrium sp. 18W-13a Accummulates High Amounts of Squalene’, Bioscience, Biotechnology, and Biochemistry, 75(11), pp. 2246–2248. doi: 10.1271/bbb.110430.
. Lee Chang, K. J. et al. (2013) ‘High cell density cultivation of a novel Aurantiochytrium sp. strain TC 20 in a fed-batch system using glycerol to produce feedstock for biodiesel and omega-3 oils’, Applied Microbiology and Biotechnology, 97(15), pp. 6907–6918. doi: 10.1007/s00253-013-4965-z.
. Lee Chang, K. J. et al. (2014) ‘Comparison of Thraustochytrids Aurantiochytrium sp., Schizochytrium sp., Thraustochytrium sp., and Ulkenia sp. for Production of Biodiesel, Long-Chain Omega-3 Oils, and Exopolysaccharide’, Marine Biotechnology, 16(4), pp. 396–411. doi: 10.1007/s10126-014-9560-5.
. Li, J. et al. (2015) ‘Biological potential of microalgae in China for biorefinery-based production of biofuels and high value compounds’, New Biotechnology, 32(6), pp. 588–596. doi: 10.1016/j.nbt.2015.02.001.
. Li, Q. et al. (2009) ‘Screening and characterization of squalene-producing thraustochytrids from Hong Kong mangroves’, Journal of Agricultural and Food Chemistry, 57(10), pp. 4267–4272. doi: 10.1021/jf9003972.
. Li, Q., Du, W. and Liu, D. (2016) ‘Perspectives of microbial oils for biodiesel production’, (2008), pp. 749–756. doi: 10.1007/s00253-008-1625-9.
. Li, X. et al. (2019) ‘Extraction and purification of eicosapentaenoic acid and docosahexaenoic acid from microalgae: A critical review’, Algal Research, 43(July). doi: 10.1016/j.algal.2019.101619.
. Lim, D. K. Y. et al. (2012) ‘Isolation and evaluation of oil-producing microalgae from subtropical coastal and Brackish waters’, PLoS ONE, 7(7). doi: 10.1371/journal.pone.0040751.
. Liu, Z. et al. (2013) ‘Isolation and characterization of a marine microalga for biofuel production with astaxanthin as a co-product’, Energies, 6(6), pp. 2759–2772. doi: 10.3390/en6062759.
. Löffelholz, C. et al. (2013) ‘Bioengineering Parameters for Single-Use Bioreactors : Overview and Evaluation of Suitable Methods’, (1), pp. 40–56. doi: 10.1002/cite.201200125.
. Morabito, C., Bournaud, C., Maës, C., Schuler, M., Aiese, R., et al. (2019) ‘Progress in Lipid Research The lipid metabolism in thraustochytrids’, Progress in Lipid Research, 76(August), p. 101007. doi: 10.1016/j.plipres.2019.101007.
. Morabito, C., Bournaud, C., Maës, C., Schuler, M., Aiese Cigliano, R., et al. (2019) ‘The lipid metabolism in thraustochytrids’, Progress in Lipid Research, 76(May), p. 101007. doi: 10.1016/j.plipres.2019.101007.
. Oslan, S. N. H. et al. (2021) ‘A review on haematococcus pluvialis bioprocess optimization of green and red stage culture conditions for the production of natural astaxanthin’, Biomolecules, 11(2), pp. 1–15. doi: 10.3390/biom11020256.
. Park, S. et al. (2017) ‘Organic solvent-free lipid extraction from wet Aurantiochytrium sp . biomass for co-production of biodiesel and value-added products’, Applied Biological Chemistry. doi: 10.1007/s13765-017-0258-z.
. Park, W. K. et al. (2018) ‘Economical DHA (Docosahexaenoic acid) production from Aurantiochytrium sp. KRS101 using orange peel extract and low cost nitrogen sources’, Algal Research, 29(November 2017), pp. 71–79. doi: 10.1016/j.algal.2017.11.017.
. Phan, T. et al. (2020) ‘Squalene Emulsion Manufacturing Process Scale-Up for Enhanced Global Pandemic Response’, Pharmaceuticals, 13, p. 168. doi: doi:10.3390/ph13080168.
. Pooja, S. (2014) ‘Algae used as Medicine and Food-A Short Review’, 6(162), pp. 33–35.
. Pradima, J., Kulkarni, M. R. and Archna (2017) ‘Review on enzymatic synthesis of value added products of glycerol, a by-product derived from biodiesel production’, Resource-Efficient Technologies, 3(4), pp. 394–405. doi: 10.1016/j.reffit.2017.02.009.
. Puri, M. (no date) ‘Algal biotechnology for pursuing omega-3 fatty acid ( bioactive ) production’, pp. 85–88.
. Quilodrán, B. et al. (2010a) ‘Docosahexaenoic acid (C22:6n-3, DHA) and astaxanthin production by Thraustochytriidae sp. AS4-A1 a native strain with high similitude to Ulkenia sp.: Evaluation of liquid residues from food industry as nutrient sources’, Enzyme and Microbial Technology, 47(1–2), pp. 24–30. doi: 10.1016/j.enzmictec.2010.04.002.
. Quilodrán, B. et al. (2010b) ‘Enzyme and Microbial Technology Docosahexaenoic acid ( C22 : 6 n − 3 , DHA ) and astaxanthin production by Thraustochytriidae sp . AS4-A1 a native strain with high similitude to Ulkenia sp .: Evaluation of liquid residues from food industry as nutrient so’, 47, pp. 24–30. doi: 10.1016/j.enzmictec.2010.04.002.
. Rani, A., Meghana, R. and Kush, A. (2018) ‘Squalene production in the cell suspension cultures of Indian sandalwood (Santalum album L.) in shake flasks and air lift bioreactor’, Plant Cell, Tissue and Organ Culture, 135(1), pp. 155–167. doi: 10.1007/s11240-018-1452-3.
. Rizwan, M. et al. (2018) ‘Exploring the potential of microalgae for new biotechnology applications and beyond : A review’, Renewable and Sustainable Energy Reviews, 92(March 2017), pp. 394–404. doi: 10.1016/j.rser.2018.04.034.
. Rosales-garcÃa, T., Jimenez-martinez, C. and Dávila-ortiz, G. (2017) ‘Squalene Extraction : Biological Sources and Extraction Methods .’, (4), pp. 1662–1670.
. Sakthivel, R., Elumalai, S. and Mohommad, M. (2011) ‘Microalgae lipid research , past , present : A critical review for biodiesel production , in the future’, 2(10), pp. 29–49.
. Sanchez, D. M., Kyndt, J. and Martinez, A. (2015) ‘Heterotrophic growth of microalgae : metabolic aspects’, pp. 1–9. doi: 10.1007/s11274-014-1773-2.
. Sarker, P. K. et al. (2016) ‘Towards Sustainable Aquafeeds : Complete Substitution of Fish Oil with Marine Microalga Schizochytrium sp . Improves Growth and Fatty Acid Deposition in Juvenile Nile Tilapia ( Oreochromis niloticus )’, pp. 1–17. doi: 10.1371/journal.pone.0156684.
. Shah, M. R. et al. (2018) ‘Microalgae in aquafeeds for a sustainable aquaculture industry’, Journal of Applied Phycology, 30(1), pp. 197–213. doi: 10.1007/s10811-017-1234-z.
. Shuaib, M. et al. (2021) ‘Materials Today : Proceedings Immunity credentials using self-sovereign identity for combating COVID-19 pandemic’, Materials Today: Proceedings, (xxxx). doi: 10.1016/j.matpr.2021.03.096.
. Singh, P. et al. (2014) ‘Ecological dynamics and biotechnological implications of thraustochytrids from marine habitats’, pp. 5789–5805. doi: 10.1007/s00253-014-5780-x.
. Srinuanpan, S., Cheirsilp, B. and Prasertsan, P. (2018) ‘Effective biogas upgrading and production of biodiesel feedstocks by strategic cultivation of oleaginous microalgae’, Energy, 148, pp. 766–774. doi: 10.1016/j.energy.2018.02.010.
. Suen, Y. L. et al. (2014) ‘Enhanced production of fatty acids and astaxanthin in Aurantiochytrium sp. by the expression of Vitreoscilla hemoglobin’, Journal of Agricultural and Food Chemistry, 62(51), pp. 12392–12398. doi: 10.1021/jf5048578.
. Suhendra et al. (2019) ‘Kajian singkat potensi rancang bangun pabrik omega-3 (DHA) kemurnian tinggi berbahan baku spesies Aurantiochytrium dari hutan bakau indonesia untuk menunjang ketahanan pangan nasional’, Konversi, 8(1), pp. 33–44.
. Suhendra (2020) Isolation of Marine Microalgae.
. Suhendra, Pantoiyo, T., et al. (2021) ‘Bioprocess Potentials of Squalene from Thraustochytrids Microalgae for Nutraceuticals in New Normal Era Isolated from Indonesian Mangroves: A Review’, CHEMICA, 8(1).
. Suhendra, Sulistiawati, E., et al. (2021) ‘Potentials of Omega-3 Rich Microalgae from Kulonprogo Mangrove Forest Yogyakarta for Nutraceuticals and Pharmaceuticals Products’, in Second International Symposium of Indonesian Chemical Engineering (2nd ISIChem 2021). Semarang, Indonesia.
. Veramis (2017) Evonik and DSM select Blair, Nebraska, as manufacturing site for innovative, new omega-3 fatty acids production, Pivotal Sources.
. Watanabe, K. et al. (2018) ‘Isolation of high carotenoid-producing aurantiochytrium sp. Mutants and improvement of astaxanthin productivity using metabolic information’, Journal of Oleo Science, 67(5), pp. 571–578. doi: 10.5650/jos.ess17230.
. Winwood, R. J. (2013a) ‘Recent developments in the commercial production’, 20(6).
. Winwood, R. J. (2013b) ‘Recent developments in the commercial production of DHA and EPA rich oils from micro-algae’, Oilseeds and Fats Crops and Lipids, 20(6). doi: 10.1052/ocl/2013030.
. Zhao, Y. et al. (2015) ‘Enhanced astaxanthin production from a novel strain of Haematococcus pluvialis using fulvic acid’, Process Biochemistry, 50(12), pp. 2072–2077. doi: 10.1016/j.procbio.2015.09.004.
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